Staphylococcus epidermidis Phages Transduce Antimicrobial
Resistance Plasmids and Mobilize Chromosomal Islands

J 2021

Staphylococcus epidermidis Phages Transduce Antimicrobial Resistance Plasmids and Mobilize Chromosomal Islands

FIŠAROVÁ, Lenka, Tibor BOTKA, Xin DU, Ivana MAŠLAŇOVÁ, Pavol BÁRDY et. al.

Základní údaje

Originální název

Staphylococcus epidermidis Phages Transduce Antimicrobial Resistance Plasmids and Mobilize Chromosomal Islands

Autoři

FIŠAROVÁ, Lenka (203 Česká republika, domácí), Tibor BOTKA (203 Česká republika, domácí), Xin DU (156 Čína), Ivana MAŠLAŇOVÁ (203 Česká republika, domácí), Pavol BÁRDY (703 Slovensko, domácí), Roman PANTŮČEK (203 Česká republika, domácí), Martin BENEŠÍK (203 Česká republika, domácí), Pavel ROUDNICKÝ (203 Česká republika, domácí), Volker WINSTEL (276 Německo), Jesper LARSEN (208 Dánsko), Ralf ROSENSTEIN (276 Německo), Andreas PESCHEL (276 Německo) a Jiří DOŠKAŘ (203 Česká republika, garant, domácí)

Vydání

mSphere, Washington, DC, USA, American Society for Microbiology, 2021, 2379-5042

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.029

Kód RIV

RIV/00216224:14310/21:00118971

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1128/mSphere.00223-21

UT WoS

000663083400008

Klíčová slova anglicky

bacteriophages; Staphylococcus epidermidis; antibiotic resistance; horizontal gene transfer; pathogenicity islands; transduction

Štítky

CF CRYO, CF GEN, CF PROT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 10. 2024 14:20, Ing. Martina Blahová

Anotace

V originále

Staphylococcus epidermidis is a leading opportunistic pathogen causing nosocomial infections that is notable for its ability to form a biofilm and for its high rates of antibiotic resistance. It serves as a reservoir of multiple antimicrobial resistance genes that spread among the staphylococcal population by horizontal gene transfer such as transduction. While phage-mediated transduction is well studied in Staphylococcus aureus, S. epidermidis transducing phages have not been described in detail yet. Here, we report the characteristics of four phages, 27, 48, 456, and 459, previously used for S. epidermidis phage typing, and the newly isolated phage E72, from a clinical S. epidermidis strain. The phages, classified in the family Siphoviridae and genus Phietavirus, exhibited an S. epidermidis-specific host range, and together they infected 49% of the 35 strains tested. A whole-genome comparison revealed evolutionary relatedness to transducing S. aureus phietaviruses. In accordance with this, all the tested phages were capable of transduction with high frequencies up to 10−4 among S. epidermidis strains from different clonal complexes. Plasmids with sizes from 4 to 19 kb encoding resistance to streptomycin, tetracycline, and chloramphenicol were transferred. We provide here the first evidence of a phage-inducible chromosomal island transfer in S. epidermidis. Similarly to S. aureus pathogenicity islands, the transfer was accompanied by phage capsid remodeling; however, the interfering protein encoded by the island was distinct. Our findings underline the role of S. epidermidis temperate phages in the evolution of S. epidermidis strains by horizontal gene transfer, which can also be utilized for S. epidermidis genetic studies.

Návaznosti

GA18-13064S, projekt VaV

Název: Analýza interakcí mezi polyvalentním terapeutickým fágem druhu Twort-like a jeho hostitelem Staphylococcus aureus

Investor: Grantová agentura ČR, Analýza interakcí mezi polyvalentním terapeutickým fágem druhu Twort-like a jeho hostitelem Staphylococcus aureus

LM2018127, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

MUNI/A/1522/2020, interní kód MU

Název: Podpora výzkumné činnosti studentů molekulární biologie a genetiky 9 (Akronym: MBG9)

Investor: Masarykova univerzita, Podpora výzkumné činnosti studentů molekulární biologie a genetiky 9

90127, velká výzkumná infrastruktura

Název: CIISB II

90132, velká výzkumná infrastruktura

Název: NCMG II

Přiložené soubory

mSphere-2021-Fisarova-e00223-21.full.pdf

Požádat o autorskou verzi souboru

Citovat

FIŠAROVÁ, Lenka, Tibor BOTKA, Xin DU, Ivana MAŠLAŇOVÁ, Pavol BÁRDY, Roman PANTŮČEK, Martin BENEŠÍK, Pavel ROUDNICKÝ, Volker WINSTEL, Jesper LARSEN, Ralf ROSENSTEIN, Andreas PESCHEL a Jiří DOŠKAŘ. Staphylococcus epidermidis Phages Transduce Antimicrobial Resistance Plasmids and Mobilize Chromosomal Islands. mSphere. Washington, DC, USA: American Society for Microbiology, 2021, roč. 6, č. 3, s. 1-19. ISSN 2379-5042. Dostupné z: https://dx.doi.org/10.1128/mSphere.00223-21.

@article{1769437,
   author = {Fišarová, Lenka and Botka, Tibor and Du, Xin and Mašlaňová, Ivana and Bárdy, Pavol and Pantůček, Roman and Benešík, Martin and Roudnický, Pavel and Winstel, Volker and Larsen, Jesper and Rosenstein, Ralf and Peschel, Andreas and Doškař, Jiří},
   article_location = {Washington, DC, USA},
   article_number = {3},
   doi = {http://dx.doi.org/10.1128/mSphere.00223-21},
   keywords = {bacteriophages; Staphylococcus epidermidis; antibiotic resistance; horizontal gene transfer; pathogenicity islands; transduction},
   language = {eng},
   issn = {2379-5042},
   journal = {mSphere},
   title = {Staphylococcus epidermidis Phages Transduce Antimicrobial Resistance Plasmids and Mobilize Chromosomal Islands},
   url = {https://journals.asm.org/doi/10.1128/mSphere.00223-21},
   volume = {6},
   year = {2021}
}

TY  - JOUR
ID  - 1769437
AU  - Fišarová, Lenka - Botka, Tibor - Du, Xin - Mašlaňová, Ivana - Bárdy, Pavol - Pantůček, Roman - Benešík, Martin - Roudnický, Pavel - Winstel, Volker - Larsen, Jesper - Rosenstein, Ralf - Peschel, Andreas - Doškař, Jiří
PY  - 2021
TI  - Staphylococcus epidermidis Phages Transduce Antimicrobial Resistance Plasmids and Mobilize Chromosomal Islands
JF  - mSphere
VL  - 6
IS  - 3
SP  - 1-19
EP  - 1-19
PB  - American Society for Microbiology
SN  - 23795042
KW  - bacteriophages
KW  - Staphylococcus epidermidis
KW  - antibiotic resistance
KW  - horizontal gene transfer
KW  - pathogenicity islands
KW  - transduction
UR  - https://journals.asm.org/doi/10.1128/mSphere.00223-21
N2  - Staphylococcus epidermidis is a leading opportunistic pathogen causing nosocomial infections that is notable for its ability to form a biofilm and for its high rates of antibiotic resistance. It serves as a reservoir of multiple antimicrobial resistance genes that spread among the staphylococcal population by horizontal gene transfer such as transduction. While phage-mediated transduction is well studied in Staphylococcus aureus, S. epidermidis transducing phages have not been described in detail yet. Here, we report the characteristics of four phages, 27, 48, 456, and 459, previously used for S. epidermidis phage typing, and the newly isolated phage E72, from a clinical S. epidermidis strain. The phages, classified in the family Siphoviridae and genus Phietavirus, exhibited an S. epidermidis-specific host range, and together they infected 49% of the 35 strains tested. A whole-genome comparison revealed evolutionary relatedness to transducing S. aureus phietaviruses. In accordance with this, all the tested phages were capable of transduction with high frequencies up to 10−4 among S. epidermidis strains from different clonal complexes. Plasmids with sizes from 4 to 19 kb encoding resistance to streptomycin, tetracycline, and chloramphenicol were transferred. We provide here the first evidence of a phage-inducible chromosomal island transfer in S. epidermidis. Similarly to S. aureus pathogenicity islands, the transfer was accompanied by phage capsid remodeling; however, the interfering protein encoded by the island was distinct. Our findings underline the role of S. epidermidis temperate phages in the evolution of S. epidermidis strains by horizontal gene transfer, which can also be utilized for S. epidermidis genetic studies.
ER  -

FIŠAROVÁ, Lenka, Tibor BOTKA, Xin DU, Ivana MAŠLAŇOVÁ, Pavol BÁRDY, Roman PANTŮČEK, Martin BENEŠÍK, Pavel ROUDNICKÝ, Volker WINSTEL, Jesper LARSEN, Ralf ROSENSTEIN, Andreas PESCHEL a Jiří DOŠKAŘ. Staphylococcus epidermidis Phages Transduce Antimicrobial Resistance Plasmids and Mobilize Chromosomal Islands. \textit{mSphere}. Washington, DC, USA: American Society for Microbiology, 2021, roč.~6, č.~3, s.~1-19. ISSN~2379-5042. Dostupné z: https://dx.doi.org/10.1128/mSphere.00223-21.

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