Smoking in women with chronic vaginal discomfort is not
associated with decreased abundance of Lactobacillus spp. but
promotes Mobiluncus and Gardnerella spp. overgrowth - secondary
analysis of trial data including microbiome analysis

J 2021

Smoking in women with chronic vaginal discomfort is not associated with decreased abundance of Lactobacillus spp. but promotes Mobiluncus and Gardnerella spp. overgrowth - secondary analysis of trial data including microbiome analysis

TUZIL, J., B. FILKOVA, J. MALINA, J. KERESTES, Tomáš DOLEŽAL et. al.

Základní údaje

Originální název

Smoking in women with chronic vaginal discomfort is not associated with decreased abundance of Lactobacillus spp. but promotes Mobiluncus and Gardnerella spp. overgrowth - secondary analysis of trial data including microbiome analysis

Název česky

Kouření u žen s chronickým vaginálním diskomfortem není spojeno se sníženým výskytem Lactobacillus spp. ale podporuje nadměrný růst bakterií Mobiluncus a Gardnerella spp. - sekundární analýza dat z klinické studie zahrnující mikrobiální analýzu

Autoři

TUZIL, J. (203 Česká republika, garant), B. FILKOVA (203 Česká republika), J. MALINA (203 Česká republika), J. KERESTES (203 Česká republika) a Tomáš DOLEŽAL (203 Česká republika, domácí)

Vydání

ČESKÁ GYNEKOLOGIE, PRAGUE, NAKLADATELSKE STREDISKO C L S J E PURKYNE, 2021, 1210-7832

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30214 Obstetrics and gynaecology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14110/21:00121696

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.48095/cccg202122

UT WoS

000643562000003

Klíčová slova anglicky

bacterial vaginosis; smoking; Nugent score; vaginal pH; vaginal fl ora; Lactobacillus; Gardnerella; Mobiluncus; human microbiome

Štítky

14110516, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 6. 2021 13:41, Mgr. Tereza Miškechová

Anotace

V originále

Background: Smoking is considered a risk factor for bacterial vaginosis. It is currently unknown which parameters of the vaginal environment are affected and how smoking triggers the disease. Aim of the study: The primary objective is to estimate the effect size of smoking on vaginal pH and the Nugent score in patients with chronic vulvovaginal discomfort prior to the development of episode of vaginosis. The secondary goal is to investigate the effect of smoking on individual microscopic parameters of the vaginal environment and on subjectively reported symptoms of vaginal discomfort. Methods: Smoking reported by patients was tested as a predictor, using multivariate logistic and ordinal logistic regression analysis on a dataset from the first visit of a randomized trial NCT04171947, which enrolled patients with intermediate vaginal environment. We tested the primary hypothesis (odds ratio (OR) for vaginal pH > 4.5 and Nugent score > 3 in smokers) at the significance level alpha = 5%. For exploratory analyses of the effect of smoking on the parameters of the vaginal environment,. was corrected as per Bonferoni. Results: In a cross-sectional sample of 250 women after adjusting for other risk factors, smoking had an impact on the Nugent score (OR = 3.3 (1.3-8.5), P = 0.011), while pH was not affected (OR = 1.2 (0.5-2.8), P = 0.698). Smoking was associated with the prevalence of clue cells (P < 0.000), Gardnerella spp. (P = 0.001) and Mobiluncus spp. (P = 0.001), while the prevalence of Lactobacillus remained unchanged (P = 0.049). Conclusion: Contrarily to common assumptions, vaginal Lactobacillus is not directly affected by smoking, which rather promotes the growth of bacteria of Gardnerella and Mobiluncus spp. Given that other parameters remained unaffected, it appears that smoking leads to vaginal dysbiosis by creating specific favourable conditions for these two opportunistic pathogens.

Citovat

TUZIL, J., B. FILKOVA, J. MALINA, J. KERESTES a Tomáš DOLEŽAL. Smoking in women with chronic vaginal discomfort is not associated with decreased abundance of Lactobacillus spp. but promotes Mobiluncus and Gardnerella spp. overgrowth - secondary analysis of trial data including microbiome analysis. ČESKÁ GYNEKOLOGIE. PRAGUE: NAKLADATELSKE STREDISKO C L S J E PURKYNE, 2021, roč. 86, č. 1, s. 22-29. ISSN 1210-7832. Dostupné z: https://dx.doi.org/10.48095/cccg202122.

@article{1773202,
   author = {Tuzil, J. and Filkova, B. and Malina, J. and Kerestes, J. and Doležal, Tomáš},
   article_location = {PRAGUE},
   article_number = {1},
   doi = {http://dx.doi.org/10.48095/cccg202122},
   keywords = {bacterial vaginosis; smoking; Nugent score; vaginal pH; vaginal fl ora; Lactobacillus; Gardnerella; Mobiluncus; human microbiome},
   language = {eng},
   issn = {1210-7832},
   journal = {ČESKÁ GYNEKOLOGIE},
   title = {Smoking in women with chronic vaginal discomfort is not associated with decreased abundance of Lactobacillus spp. but promotes Mobiluncus and Gardnerella spp. overgrowth - secondary analysis of trial data including microbiome analysis},
   url = {https://www.prolekare.cz/casopisy/ceska-gynekologie/2021-1-12/koureni-u-zen-s-chronickym-vaginalnim-diskomfortem-neni-spojeno-se-snizenym-vyskytem-lactobacillus-spp-ale-podporuje-nadmerny-rust-bakterii-mobiluncus-a-gardnerella-spp-sekundarni-analyza-dat-},
   volume = {86},
   year = {2021}
}

TY  - JOUR
ID  - 1773202
AU  - Tuzil, J. - Filkova, B. - Malina, J. - Kerestes, J. - Doležal, Tomáš
PY  - 2021
TI  - Smoking in women with chronic vaginal discomfort is not associated with decreased abundance of Lactobacillus spp. but promotes Mobiluncus and Gardnerella spp. overgrowth - secondary analysis of trial data including microbiome analysis
JF  - ČESKÁ GYNEKOLOGIE
VL  - 86
IS  - 1
SP  - 22-29
EP  - 22-29
PB  - NAKLADATELSKE STREDISKO C L S J E PURKYNE
SN  - 12107832
KW  - bacterial vaginosis
KW  - smoking
KW  - Nugent score
KW  - vaginal pH
KW  - vaginal fl ora
KW  - Lactobacillus
KW  - Gardnerella
KW  - Mobiluncus
KW  - human microbiome
UR  - https://www.prolekare.cz/casopisy/ceska-gynekologie/2021-1-12/koureni-u-zen-s-chronickym-vaginalnim-diskomfortem-neni-spojeno-se-snizenym-vyskytem-lactobacillus-spp-ale-podporuje-nadmerny-rust-bakterii-mobiluncus-a-gardnerella-spp-sekundarni-analyza-dat-
N2  - Background: Smoking is considered a risk factor for bacterial vaginosis. It is currently unknown which parameters of the vaginal environment are affected and how smoking triggers the disease. Aim of the study: The primary objective is to estimate the effect size of smoking on vaginal pH and the Nugent score in patients with chronic vulvovaginal discomfort prior to the development of episode of vaginosis. The secondary goal is to investigate the effect of smoking on individual microscopic parameters of the vaginal environment and on subjectively reported symptoms of vaginal discomfort. Methods: Smoking reported by patients was tested as a predictor, using multivariate logistic and ordinal logistic regression analysis on a dataset from the first visit of a randomized trial NCT04171947, which enrolled patients with intermediate vaginal environment. We tested the primary hypothesis (odds ratio (OR) for vaginal pH > 4.5 and Nugent score > 3 in smokers) at the significance level alpha = 5%. For exploratory analyses of the effect of smoking on the parameters of the vaginal environment,. was corrected as per Bonferoni. Results: In a cross-sectional sample of 250 women after adjusting for other risk factors, smoking had an impact on the Nugent score (OR = 3.3 (1.3-8.5), P = 0.011), while pH was not affected (OR = 1.2 (0.5-2.8), P = 0.698). Smoking was associated with the prevalence of clue cells (P < 0.000), Gardnerella spp. (P = 0.001) and Mobiluncus spp. (P = 0.001), while the prevalence of Lactobacillus remained unchanged (P = 0.049). Conclusion: Contrarily to common assumptions, vaginal Lactobacillus is not directly affected by smoking, which rather promotes the growth of bacteria of Gardnerella and Mobiluncus spp. Given that other parameters remained unaffected, it appears that smoking leads to vaginal dysbiosis by creating specific favourable conditions for these two opportunistic pathogens.
ER  -

TUZIL, J., B. FILKOVA, J. MALINA, J. KERESTES a Tomáš DOLEŽAL. Smoking in women with chronic vaginal discomfort is not associated with decreased abundance of Lactobacillus spp. but promotes Mobiluncus and Gardnerella spp. overgrowth - secondary analysis of trial data including microbiome analysis. \textit{ČESKÁ GYNEKOLOGIE}. PRAGUE: NAKLADATELSKE STREDISKO C L S J E PURKYNE, 2021, roč.~86, č.~1, s.~22-29. ISSN~1210-7832. Dostupné z: https://dx.doi.org/10.48095/cccg202122.

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