SiO2-Decorated Parylene C Micropillars Designed to Probe
Cellular Force

J 2021

SiO2-Decorated Parylene C Micropillars Designed to Probe Cellular Force

FOHLEROVÁ, Zdenka, Imrich GABLECH, Alexandr OTAHAL a Peter FECKO

Základní údaje

Originální název

SiO2-Decorated Parylene C Micropillars Designed to Probe Cellular Force

Autoři

FOHLEROVÁ, Zdenka (203 Česká republika, garant, domácí), Imrich GABLECH (203 Česká republika), Alexandr OTAHAL (203 Česká republika) a Peter FECKO (703 Slovensko)

Vydání

ADVANCED MATERIALS INTERFACES, HOBOKEN, WILEY, 2021, 2196-7350

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10404 Polymer science

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.389

Kód RIV

RIV/00216224:14110/21:00121732

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1002/admi.202001897

UT WoS

000611098000001

Klíčová slova anglicky

cellular force; mechanobiology; micropillars; parylene C; silanization; silicon dioxide

Štítky

14110512, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 8. 6. 2021 13:00, Mgr. Tereza Miškechová

Anotace

V originále

Living cells sense and respond to mechanical signals through specific mechanisms generating traction force. The quantification of cell forces using micropillars can be limited by micropillar stiffness, technological aspects of fabrications, and microcontact printing of proteins. This paper develops the new design of SiO2/Parylene C micropillars with an aspect ratio of 6 and 3.5 and spring constant of 4.7 and 28 mu N mu m(-1), respectively. The upper part of micropillars is coated with a 250 nm layer of SiO2, and results confirm protein deposition on individual micropillars via SiO2 interface and non-adhesiveness on the micropillars' sidewalls. Results show an absence of cytotoxicity for micropillar-based substrates and a dependence on its stiffness. Stiffer micropillars enhance cell adhesion and proliferation rate, and a stronger cellular force of approximate to 25 mu N is obtained. The main contribution of SiO2/parylene C micropillars is the elimination of the step involving the fabrication of polydimethylsiloxane stamp because the array enables covalent binding of proteins via SiO2 chemistry. These micropillars stand on Si wafer and thus, any warping of underlying polymer membrane does not have to be considered. Additionally, SiO2/parylene C micropillars can broaden the range of stiffer substrates to be probed by cells.

Citovat

FOHLEROVÁ, Zdenka, Imrich GABLECH, Alexandr OTAHAL a Peter FECKO. SiO2-Decorated Parylene C Micropillars Designed to Probe Cellular Force. ADVANCED MATERIALS INTERFACES. HOBOKEN: WILEY, 2021, roč. 8, č. 6, s. 1-8. ISSN 2196-7350. Dostupné z: https://dx.doi.org/10.1002/admi.202001897.

@article{1774698,
   author = {Fohlerová, Zdenka and Gablech, Imrich and Otahal, Alexandr and Fecko, Peter},
   article_location = {HOBOKEN},
   article_number = {6},
   doi = {http://dx.doi.org/10.1002/admi.202001897},
   keywords = {cellular force; mechanobiology; micropillars; parylene C; silanization; silicon dioxide},
   language = {eng},
   issn = {2196-7350},
   journal = {ADVANCED MATERIALS INTERFACES},
   title = {SiO2-Decorated Parylene C Micropillars Designed to Probe Cellular Force},
   url = {https://onlinelibrary.wiley.com/doi/epdf/10.1002/admi.202001897},
   volume = {8},
   year = {2021}
}

TY  - JOUR
ID  - 1774698
AU  - Fohlerová, Zdenka - Gablech, Imrich - Otahal, Alexandr - Fecko, Peter
PY  - 2021
TI  - SiO2-Decorated Parylene C Micropillars Designed to Probe Cellular Force
JF  - ADVANCED MATERIALS INTERFACES
VL  - 8
IS  - 6
SP  - 1-8
EP  - 1-8
PB  - WILEY
SN  - 21967350
KW  - cellular force
KW  - mechanobiology
KW  - micropillars
KW  - parylene C
KW  - silanization
KW  - silicon dioxide
UR  - https://onlinelibrary.wiley.com/doi/epdf/10.1002/admi.202001897
N2  - Living cells sense and respond to mechanical signals through specific mechanisms generating traction force. The quantification of cell forces using micropillars can be limited by micropillar stiffness, technological aspects of fabrications, and microcontact printing of proteins. This paper develops the new design of SiO2/Parylene C micropillars with an aspect ratio of 6 and 3.5 and spring constant of 4.7 and 28 mu N mu m(-1), respectively. The upper part of micropillars is coated with a 250 nm layer of SiO2, and results confirm protein deposition on individual micropillars via SiO2 interface and non-adhesiveness on the micropillars' sidewalls. Results show an absence of cytotoxicity for micropillar-based substrates and a dependence on its stiffness. Stiffer micropillars enhance cell adhesion and proliferation rate, and a stronger cellular force of approximate to 25 mu N is obtained. The main contribution of SiO2/parylene C micropillars is the elimination of the step involving the fabrication of polydimethylsiloxane stamp because the array enables covalent binding of proteins via SiO2 chemistry. These micropillars stand on Si wafer and thus, any warping of underlying polymer membrane does not have to be considered. Additionally, SiO2/parylene C micropillars can broaden the range of stiffer substrates to be probed by cells.
ER  -

FOHLEROVÁ, Zdenka, Imrich GABLECH, Alexandr OTAHAL a Peter FECKO. SiO2-Decorated Parylene C Micropillars Designed to Probe Cellular Force. \textit{ADVANCED MATERIALS INTERFACES}. HOBOKEN: WILEY, 2021, roč.~8, č.~6, s.~1-8. ISSN~2196-7350. Dostupné z: https://dx.doi.org/10.1002/admi.202001897.

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