Dynamic miRNA changes during the process of epileptogenesis in
an infantile and adult-onset model

J 2021

Dynamic miRNA changes during the process of epileptogenesis in an infantile and adult-onset model

BENCÚROVÁ, Petra, Jiří BALOUN, Jakub HYNŠT, Jan OPPELT, Hana KUBOVÁ et. al.

Základní údaje

Originální název

Dynamic miRNA changes during the process of epileptogenesis in an infantile and adult-onset model

Autoři

BENCÚROVÁ, Petra (703 Slovensko, domácí), Jiří BALOUN (203 Česká republika, domácí), Jakub HYNŠT (203 Česká republika, domácí), Jan OPPELT (203 Česká republika, domácí), Hana KUBOVÁ (203 Česká republika), Šárka POSPÍŠILOVÁ (203 Česká republika, garant, domácí) a Milan BRÁZDIL (203 Česká republika, domácí)

Vydání

Nature Scientific Reports, London, NATURE RESEARCH, 2021, 2045-2322

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10700 1.7 Other natural sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.996

Kód RIV

RIV/00216224:14110/21:00119034

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1038/s41598-021-89084-9

UT WoS

000656986200006

Klíčová slova anglicky

Dynamic miRNA changes; epileptogenesis; infantile and adult-onset model

Štítky

14110127, CF BIOIT, CF GEN, podil, rivok

Příznaky

Recenzováno

Změněno: 15. 10. 2024 14:06, Ing. Martina Blahová

Anotace

V originále

Temporal lobe epilepsy (TLE) is the most common epilepsy type. TLE onset in infancy aggravates features like severity, drug responsiveness, or development of comorbidities. These aggravations may arise from altered micro RNA (miRNA) expression specific to the early onset of the disease. Although the miRNA involvement in TLE is widely studied, the relationship between the onset-age and miRNA expression has not been addressed. Here, we investigated the miRNA profile of infantile and adult-onset TLE in rats combining sequencing and PCR. Since miRNA expression changes with the disease progression, we scrutinized miRNA dynamics across three stages: acute, latent, and chronic. We report that infantile-onset TLE leads to changes in the expression of fewer miRNAs across these stages. Interestingly, the miRNA profile in the acute stage of infantile-onset TLE overlaps in dysregulation of miR-132-5p, -205, and -211-3p with the chronic stage of the disease starting in adulthood. The analysis of putative targets linked the majority of dysregulated miRNAs with pathways involved in epilepsy. Our profiling uncovered miRNA expression characteristic for infantile and adulthood-onset epileptogenesis, suggesting the distinct biology underlying TLE in the onset age-dependent matter. Our results indicate the necessity of addressing the onset age as an important parameter in future epilepsy research.

Návaznosti

GA16-04726S, projekt VaV

Název: miRNA a biochemické dráhy v epilepsii

Investor: Grantová agentura ČR, miRNA a biochemické dráhy v epilepsii

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

MUNI/A/1595/2020, interní kód MU

Název: Nové přístupy ve výzkumu, diagnostice a terapii hematologických malignit VIII (Akronym: VýDiTeHeMa VIII)

Investor: Masarykova univerzita, Nové přístupy ve výzkumu, diagnostice a terapii hematologických malignit VIII

90091, velká výzkumná infrastruktura

Název: NCMG

Citovat

BENCÚROVÁ, Petra, Jiří BALOUN, Jakub HYNŠT, Jan OPPELT, Hana KUBOVÁ, Šárka POSPÍŠILOVÁ a Milan BRÁZDIL. Dynamic miRNA changes during the process of epileptogenesis in an infantile and adult-onset model. Nature Scientific Reports. London: NATURE RESEARCH, 2021, roč. 11, č. 1, s. 1-14. ISSN 2045-2322. Dostupné z: https://dx.doi.org/10.1038/s41598-021-89084-9.

@article{1779390,
   author = {Bencúrová, Petra and Baloun, Jiří and Hynšt, Jakub and Oppelt, Jan and Kubová, Hana and Pospíšilová, Šárka and Brázdil, Milan},
   article_location = {London},
   article_number = {1},
   doi = {http://dx.doi.org/10.1038/s41598-021-89084-9},
   keywords = {Dynamic miRNA changes; epileptogenesis; infantile and adult-onset model},
   language = {eng},
   issn = {2045-2322},
   journal = {Nature Scientific Reports},
   title = {Dynamic miRNA changes during the process of epileptogenesis in an infantile and adult-onset model},
   url = {https://www.nature.com/articles/s41598-021-89084-9.pdf},
   volume = {11},
   year = {2021}
}

TY  - JOUR
ID  - 1779390
AU  - Bencúrová, Petra - Baloun, Jiří - Hynšt, Jakub - Oppelt, Jan - Kubová, Hana - Pospíšilová, Šárka - Brázdil, Milan
PY  - 2021
TI  - Dynamic miRNA changes during the process of epileptogenesis in an infantile and adult-onset model
JF  - Nature Scientific Reports
VL  - 11
IS  - 1
SP  - 1-14
EP  - 1-14
PB  - NATURE RESEARCH
SN  - 20452322
KW  - Dynamic miRNA changes
KW  - epileptogenesis
KW  - infantile and adult-onset model
UR  - https://www.nature.com/articles/s41598-021-89084-9.pdf
N2  - Temporal lobe epilepsy (TLE) is the most common epilepsy type. TLE onset in infancy aggravates features like severity, drug responsiveness, or development of comorbidities. These aggravations may arise from altered micro RNA (miRNA) expression specific to the early onset of the disease. Although the miRNA involvement in TLE is widely studied, the relationship between the onset-age and miRNA expression has not been addressed. Here, we investigated the miRNA profile of infantile and adult-onset TLE in rats combining sequencing and PCR. Since miRNA expression changes with the disease progression, we scrutinized miRNA dynamics across three stages: acute, latent, and chronic. We report that infantile-onset TLE leads to changes in the expression of fewer miRNAs across these stages. Interestingly, the miRNA profile in the acute stage of infantile-onset TLE overlaps in dysregulation of miR-132-5p, -205, and -211-3p with the chronic stage of the disease starting in adulthood. The analysis of putative targets linked the majority of dysregulated miRNAs with pathways involved in epilepsy. Our profiling uncovered miRNA expression characteristic for infantile and adulthood-onset epileptogenesis, suggesting the distinct biology underlying TLE in the onset age-dependent matter. Our results indicate the necessity of addressing the onset age as an important parameter in future epilepsy research.
ER  -

BENCÚROVÁ, Petra, Jiří BALOUN, Jakub HYNŠT, Jan OPPELT, Hana KUBOVÁ, Šárka POSPÍŠILOVÁ a Milan BRÁZDIL. Dynamic miRNA changes during the process of epileptogenesis in an infantile and adult-onset model. \textit{Nature Scientific Reports}. London: NATURE RESEARCH, 2021, roč.~11, č.~1, s.~1-14. ISSN~2045-2322. Dostupné z: https://dx.doi.org/10.1038/s41598-021-89084-9.

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