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@inproceedings{1783820,
author = {Zemánková, Kristýna and Pavelicová, Kristýna and Bezděková, Jaroslava and Nejdl, Lukáš and Vejvodová, Markéta and Vaculovič, Tomáš and Vaculovičová, Markéta},
address = {Ostrava},
booktitle = {NANOCON 2020: 12th International Conference on Nanomaterials - Research & Application},
doi = {http://dx.doi.org/10.37904/nanocon.2020.3749},
keywords = {Metallothionein; Molecularly imprinted polymers; Magnetic particles; LA-ICP-MS},
howpublished = {elektronická verze "online"},
language = {eng},
location = {Ostrava},
isbn = {978-80-87294-98-7},
pages = {385-389},
publisher = {TANGER Ltd.},
title = {Mettalothionein sensing using molecularly imprinted polymers coupled laser ablation inductively coupled plasma-mass spectrometry},
url = {https://doi.org/10.37904/nanocon.2020.3749},
year = {2021}
}
TY - JOUR
ID - 1783820
AU - Zemánková, Kristýna - Pavelicová, Kristýna - Bezděková, Jaroslava - Nejdl, Lukáš - Vejvodová, Markéta - Vaculovič, Tomáš - Vaculovičová, Markéta
PY - 2021
TI - Mettalothionein sensing using molecularly imprinted polymers coupled laser ablation inductively coupled plasma-mass spectrometry
PB - TANGER Ltd.
CY - Ostrava
SN - 9788087294987
KW - Metallothionein
KW - Molecularly imprinted polymers
KW - Magnetic particles
KW - LA-ICP-MS
UR - https://doi.org/10.37904/nanocon.2020.3749
N2 - In this work, we report a facile method for a detection of mettalothionein (MT) - potential biomarker of tumor diseases. Combination of laser ablation inductively coupled plasma coupled to mass spectrometric detection (LA-ICP-MS) with sample pretreatment by magnetic particles modified by molecularly imprinted polymers (Mag-MIP) enables selective and sensitive protein detection and quantification. After Mag-MIP MT extraction, elements specific for the protein (S, Zn and Cd) were detected in healthy pig skin and pig melanoma tissue using MeLiM (Melanoma-bearing-Libechov-Minipig) model. It was found that levels of detected elements were significantly elevated in melanoma tissue compared to healthy skin. Currently, the investigation of MT dimerization in cancer progression is carried out taking advantage from easily controllable in vitro MT dimerization which is used for simple preparation of selective MIP extraction phase. Unlike other recognition elements, which are not always available for target analyte (e.g. antibodies), MIPs can be easily prepared as required. Moreover, magnetic particles as a MIP substrate enable elegant extraction approach and LA-ICP-MS provides extremely sensitive detection. Therefore, Mag-MIP-LA-ICP-MS is a unique cross-border combination of molecular and elemental analysis.
ER -
ZEMÁNKOVÁ, Kristýna, Kristýna PAVELICOVÁ, Jaroslava BEZDĚKOVÁ, Lukáš NEJDL, Markéta VEJVODOVÁ, Tomáš VACULOVIČ a Markéta VACULOVIČOVÁ. Mettalothionein sensing using molecularly imprinted polymers coupled laser ablation inductively coupled plasma-mass spectrometry. Online. In \textit{NANOCON 2020: 12th International Conference on Nanomaterials - Research \&{} Application}. Ostrava: TANGER Ltd., 2021, s.~385-389. ISBN~978-80-87294-98-7. Dostupné z: https://dx.doi.org/10.37904/nanocon.2020.3749.
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