Extraordinary diversity of telomeres, telomerase RNAs and their
template regions in Saccharomycetaceae

J 2021

Extraordinary diversity of telomeres, telomerase RNAs and their template regions in Saccharomycetaceae

PEŠKA, Vratislav, Petr FAJKUS, Michal BUBENÍK, Václav BRÁZDA, Natália BOHÁLOVÁ et. al.

Základní údaje

Originální název

Extraordinary diversity of telomeres, telomerase RNAs and their template regions in Saccharomycetaceae

Autoři

PEŠKA, Vratislav (203 Česká republika, garant), Petr FAJKUS (203 Česká republika, domácí), Michal BUBENÍK (203 Česká republika, domácí), Václav BRÁZDA (203 Česká republika), Natália BOHÁLOVÁ (703 Slovensko, domácí), Vojtěch DVOŘÁČEK (203 Česká republika), Jiří FAJKUS (203 Česká republika, domácí) a Sonia GARCIA (724 Španělsko)

Vydání

Scientific Reports, Nature Research, 2021, 2045-2322

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10603 Genetics and heredity

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.996

Kód RIV

RIV/00216224:14740/21:00119109

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1038/s41598-021-92126-x

UT WoS

000664915500010

Klíčová slova anglicky

DNA END-REPLICATION; SECONDARY STRUCTURE; G-QUADRUPLEXES SEQUENCES; REPEATS IDENTIFICATION; EVOLUTION; GENOME

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 9. 6. 2022 11:22, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Telomerase RNA (TR) carries the template for synthesis of telomere DNA and provides a scaffold for telomerase assembly. Fungal TRs are long and have been compared to higher eukaryotes, where they show considerable diversity within phylogenetically close groups. TRs of several Saccharomycetaceae were recently identified, however, many of these remained uncharacterised in the template region. Here we show that this is mainly due to high variability in telomere sequence. We predicted the telomere sequences using Tandem Repeats Finder and then we identified corresponding putative template regions in TR candidates. Remarkably long telomere units and the corresponding putative TRs were found in Tetrapisispora species. Notably, variable lengths of the annealing sequence of the template region (1-10 nt) were found. Consequently, species with the same telomere sequence may not harbour identical TR templates. Thus, TR sequence alone can be used to predict a template region and telomere sequence, but not to determine these exactly. A conserved feature of telomere sequences, tracts of adjacent Gs, led us to test the propensity of individual telomere sequences to form G4. The results show highly diverse values of G4-propensity, indicating the lack of ubiquitous conservation of this feature across Saccharomycetaceae.

Návaznosti

GX20-01331X, projekt VaV

Název: Biogeneze a evoluce telomerázy u rostlin

Investor: Grantová agentura ČR, Biogenesis and evolution of telomerase in plants

LM2018140, projekt VaV

Název: e-Infrastruktura CZ (Akronym: e-INFRA CZ)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, e-Infrastruktura CZ

Citovat

PEŠKA, Vratislav, Petr FAJKUS, Michal BUBENÍK, Václav BRÁZDA, Natália BOHÁLOVÁ, Vojtěch DVOŘÁČEK, Jiří FAJKUS a Sonia GARCIA. Extraordinary diversity of telomeres, telomerase RNAs and their template regions in Saccharomycetaceae. Scientific Reports. Nature Research, 2021, roč. 11, č. 1, s. "12784", 11 s. ISSN 2045-2322. Dostupné z: https://dx.doi.org/10.1038/s41598-021-92126-x.

@article{1784130,
   author = {Peška, Vratislav and Fajkus, Petr and Bubeník, Michal and Brázda, Václav and Bohálová, Natália and Dvořáček, Vojtěch and Fajkus, Jiří and Garcia, Sonia},
   article_number = {1},
   doi = {http://dx.doi.org/10.1038/s41598-021-92126-x},
   keywords = {DNA END-REPLICATION; SECONDARY STRUCTURE; G-QUADRUPLEXES SEQUENCES; REPEATS IDENTIFICATION; EVOLUTION; GENOME},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific Reports},
   title = {Extraordinary diversity of telomeres, telomerase RNAs and their template regions in Saccharomycetaceae},
   url = {https://www.nature.com/articles/s41598-021-92126-x},
   volume = {11},
   year = {2021}
}

TY  - JOUR
ID  - 1784130
AU  - Peška, Vratislav - Fajkus, Petr - Bubeník, Michal - Brázda, Václav - Bohálová, Natália - Dvořáček, Vojtěch - Fajkus, Jiří - Garcia, Sonia
PY  - 2021
TI  - Extraordinary diversity of telomeres, telomerase RNAs and their template regions in Saccharomycetaceae
JF  - Scientific Reports
VL  - 11
IS  - 1
SP  - "12784"
EP  - "12784"
PB  - Nature Research
SN  - 20452322
KW  - DNA END-REPLICATION
KW  - SECONDARY STRUCTURE
KW  - G-QUADRUPLEXES SEQUENCES
KW  - REPEATS IDENTIFICATION
KW  - EVOLUTION
KW  - GENOME
UR  - https://www.nature.com/articles/s41598-021-92126-x
N2  - Telomerase RNA (TR) carries the template for synthesis of telomere DNA and provides a scaffold for telomerase assembly. Fungal TRs are long and have been compared to higher eukaryotes, where they show considerable diversity within phylogenetically close groups. TRs of several Saccharomycetaceae were recently identified, however, many of these remained uncharacterised in the template region. Here we show that this is mainly due to high variability in telomere sequence. We predicted the telomere sequences using Tandem Repeats Finder and then we identified corresponding putative template regions in TR candidates. Remarkably long telomere units and the corresponding putative TRs were found in Tetrapisispora species. Notably, variable lengths of the annealing sequence of the template region (1-10 nt) were found. Consequently, species with the same telomere sequence may not harbour identical TR templates. Thus, TR sequence alone can be used to predict a template region and telomere sequence, but not to determine these exactly. A conserved feature of telomere sequences, tracts of adjacent Gs, led us to test the propensity of individual telomere sequences to form G4. The results show highly diverse values of G4-propensity, indicating the lack of ubiquitous conservation of this feature across Saccharomycetaceae.
ER  -

PEŠKA, Vratislav, Petr FAJKUS, Michal BUBENÍK, Václav BRÁZDA, Natália BOHÁLOVÁ, Vojtěch DVOŘÁČEK, Jiří FAJKUS a Sonia GARCIA. Extraordinary diversity of telomeres, telomerase RNAs and their template regions in Saccharomycetaceae. \textit{Scientific Reports}. Nature Research, 2021, roč.~11, č.~1, s.~''12784'', 11 s. ISSN~2045-2322. Dostupné z: https://dx.doi.org/10.1038/s41598-021-92126-x.

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