Microbiome analysis and predicted relative metabolomic turnover
suggest bacterial heme and selenium metabolism are altered in
the gastrointestinal system of zebrafish (Danio rerio) exposed
to the organochlorine dieldrin

J 2021

Microbiome analysis and predicted relative metabolomic turnover suggest bacterial heme and selenium metabolism are altered in the gastrointestinal system of zebrafish (Danio rerio) exposed to the organochlorine dieldrin

HUA, Qing, Ondřej ADAMOVSKÝ, Hana VESPALCOVÁ, Jonna BOYDA, Jordan T. SCHMIDT et. al.

Základní údaje

Originální název

Microbiome analysis and predicted relative metabolomic turnover suggest bacterial heme and selenium metabolism are altered in the gastrointestinal system of zebrafish (Danio rerio) exposed to the organochlorine dieldrin

Autoři

HUA, Qing (156 Čína), Ondřej ADAMOVSKÝ (203 Česká republika, garant, domácí), Hana VESPALCOVÁ (203 Česká republika, domácí), Jonna BOYDA (840 Spojené státy), Jordan T. SCHMIDT (840 Spojené státy), Marianne KOZUCH (840 Spojené státy), Serena L. M. CRAFT (840 Spojené státy), Pamela E. GINN (840 Spojené státy), Stanislav SMATANA (703 Slovensko, domácí), Eva BUDINSKÁ (703 Slovensko, domácí), Maria PERSICO (380 Itálie, domácí), Joseph H., Jr. BISESI (840 Spojené státy) a Christopher J. MARTYNIUK (840 Spojené státy)

Vydání

Environmental Pollution, Elsevier Ltd. 2021, 0269-7491

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10511 Environmental sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 9.988

Kód RIV

RIV/00216224:14310/21:00122022

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.envpol.2020.115715

UT WoS

000600560400034

Klíčová slova anglicky

Microbiome; Pesticide; Aquatic; Ecotoxicology; Pathology

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 1. 11. 2021 15:55, Mgr. Marie Šípková, DiS.

Anotace

V originále

Dietary exposure to chemicals alters the diversity of microbiome communities and can lead to pathophysiological changes in the gastrointestinal system. The organochlorine pesticide dieldrin is a persistent environmental contaminant that bioaccumulates in fatty tissue of aquatic organisms. The objectives of this study were to determine whether environmentally-relevant doses of dieldrin altered gastrointestinal morphology and the microbiome of zebrafish. Adult zebrafish at similar to 4 months of age were fed a measured amount of feed containing either a solvent control or one of two doses of dieldrin (measured at 16, and 163.5 ng/g dry weight) for 4 months. Dieldrin body burden levels in zebrafish after four-month exposure were 0 (control), 11.47 +/- 1.13 ng/g (low dose) and 18.32 +/- 1.32 ng/g (high dose) wet weight [mean +/- std]. Extensive histopathology at the whole organism level revealed that dieldrin exposure did not induce notable tissue pathology, including the gastrointestinal tract. A repeated measure mixed model analysis revealed that, while fish gained weight over time, there were no dieldrin-specific effects on body weight. Fecal content was collected from the gastrointestinal tract of males and 16S rRNA gene sequencing conducted. Dieldrin at a measured feed dose of 16 ng/g reduced the abundance of Firmicutes, a phylum involved in energy resorption. At the level of class, there was a decrease in abundance of Clostridia and Betaproteobacteria, and an increase in Verrucomicrobiae species. We used a computational approach called predicted relative metabolomic turnover (PRMT) to predict how a shift in microbial community composition affects exchange of metabolites. Dieldrinwas predicted to affect metabolic turnover of uroporphyrinogen I and coproporphyrinogen I [enzyme]-cysteine, hydrogen selenide, selenite, and methyl-selenic acid in the fish gastrointestinal system. These pathways are related to bacterial heme biosynthesis and selenium metabolism. Our study demonstrates that dietary exposures to dieldrin can alter microbiota composition over 4 months, however the long-term consequences of such impacts are not well understood.

Návaznosti

EF17_043/0009632, projekt VaV

Název: CETOCOEN Excellence

LM2018121, projekt VaV

Název: Výzkumná infrastruktura RECETOX (Akronym: RECETOX RI)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, RECETOX RI

857560, interní kód MU
(Kód CEP: EF17_043/0009632)

Název: CETOCOEN Excellence (Akronym: CETOCOEN Excellence)

Investor: Evropská unie, CETOCOEN Excellence, Spreading excellence and widening participation

Citovat

HUA, Qing, Ondřej ADAMOVSKÝ, Hana VESPALCOVÁ, Jonna BOYDA, Jordan T. SCHMIDT, Marianne KOZUCH, Serena L. M. CRAFT, Pamela E. GINN, Stanislav SMATANA, Eva BUDINSKÁ, Maria PERSICO, Joseph H., Jr. BISESI a Christopher J. MARTYNIUK. Microbiome analysis and predicted relative metabolomic turnover suggest bacterial heme and selenium metabolism are altered in the gastrointestinal system of zebrafish (Danio rerio) exposed to the organochlorine dieldrin. Environmental Pollution. Elsevier Ltd., 2021, roč. 268, January 2021, s. 1-11. ISSN 0269-7491. Dostupné z: https://dx.doi.org/10.1016/j.envpol.2020.115715.

@article{1784557,
   author = {Hua, Qing and Adamovský, Ondřej and Vespalcová, Hana and Boyda, Jonna and Schmidt, Jordan T. and Kozuch, Marianne and Craft, Serena L. M. and Ginn, Pamela E. and Smatana, Stanislav and Budinská, Eva and Persico, Maria and Bisesi, Joseph H., Jr. and Martyniuk, Christopher J.},
   article_number = {January 2021},
   doi = {http://dx.doi.org/10.1016/j.envpol.2020.115715},
   keywords = {Microbiome; Pesticide; Aquatic; Ecotoxicology; Pathology},
   language = {eng},
   issn = {0269-7491},
   journal = {Environmental Pollution},
   title = {Microbiome analysis and predicted relative metabolomic turnover suggest bacterial heme and selenium metabolism are altered in the gastrointestinal system of zebrafish (Danio rerio) exposed to the organochlorine dieldrin},
   url = {https://www.sciencedirect.com/science/article/pii/S0269749120364046?via%3Dihub},
   volume = {268},
   year = {2021}
}

TY  - JOUR
ID  - 1784557
AU  - Hua, Qing - Adamovský, Ondřej - Vespalcová, Hana - Boyda, Jonna - Schmidt, Jordan T. - Kozuch, Marianne - Craft, Serena L. M. - Ginn, Pamela E. - Smatana, Stanislav - Budinská, Eva - Persico, Maria - Bisesi, Joseph H., Jr. - Martyniuk, Christopher J.
PY  - 2021
TI  - Microbiome analysis and predicted relative metabolomic turnover suggest bacterial heme and selenium metabolism are altered in the gastrointestinal system of zebrafish (Danio rerio) exposed to the organochlorine dieldrin
JF  - Environmental Pollution
VL  - 268
IS  - January 2021
SP  - 1-11
EP  - 1-11
PB  - Elsevier Ltd.
SN  - 02697491
KW  - Microbiome
KW  - Pesticide
KW  - Aquatic
KW  - Ecotoxicology
KW  - Pathology
UR  - https://www.sciencedirect.com/science/article/pii/S0269749120364046?via%3Dihub
N2  - Dietary exposure to chemicals alters the diversity of microbiome communities and can lead to pathophysiological changes in the gastrointestinal system. The organochlorine pesticide dieldrin is a persistent environmental contaminant that bioaccumulates in fatty tissue of aquatic organisms. The objectives of this study were to determine whether environmentally-relevant doses of dieldrin altered gastrointestinal morphology and the microbiome of zebrafish. Adult zebrafish at similar to 4 months of age were fed a measured amount of feed containing either a solvent control or one of two doses of dieldrin (measured at 16, and 163.5 ng/g dry weight) for 4 months. Dieldrin body burden levels in zebrafish after four-month exposure were 0 (control), 11.47 +/- 1.13 ng/g (low dose) and 18.32 +/- 1.32 ng/g (high dose) wet weight [mean +/- std]. Extensive histopathology at the whole organism level revealed that dieldrin exposure did not induce notable tissue pathology, including the gastrointestinal tract. A repeated measure mixed model analysis revealed that, while fish gained weight over time, there were no dieldrin-specific effects on body weight. Fecal content was collected from the gastrointestinal tract of males and 16S rRNA gene sequencing conducted. Dieldrin at a measured feed dose of 16 ng/g reduced the abundance of Firmicutes, a phylum involved in energy resorption. At the level of class, there was a decrease in abundance of Clostridia and Betaproteobacteria, and an increase in Verrucomicrobiae species. We used a computational approach called predicted relative metabolomic turnover (PRMT) to predict how a shift in microbial community composition affects exchange of metabolites. Dieldrinwas predicted to affect metabolic turnover of uroporphyrinogen I and coproporphyrinogen I [enzyme]-cysteine, hydrogen selenide, selenite, and methyl-selenic acid in the fish gastrointestinal system. These pathways are related to bacterial heme biosynthesis and selenium metabolism. Our study demonstrates that dietary exposures to dieldrin can alter microbiota composition over 4 months, however the long-term consequences of such impacts are not well understood.
ER  -

HUA, Qing, Ondřej ADAMOVSKÝ, Hana VESPALCOVÁ, Jonna BOYDA, Jordan T. SCHMIDT, Marianne KOZUCH, Serena L. M. CRAFT, Pamela E. GINN, Stanislav SMATANA, Eva BUDINSKÁ, Maria PERSICO, Joseph H., Jr. BISESI a Christopher J. MARTYNIUK. Microbiome analysis and predicted relative metabolomic turnover suggest bacterial heme and selenium metabolism are altered in the gastrointestinal system of zebrafish (Danio rerio) exposed to the organochlorine dieldrin. \textit{Environmental Pollution}. Elsevier Ltd., 2021, roč.~268, January 2021, s.~1-11. ISSN~0269-7491. Dostupné z: https://dx.doi.org/10.1016/j.envpol.2020.115715.

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