Neither microcystin, nor nodularin, nor cylindrospermopsin
directly interact with human toll-like receptors

J 2021

Neither microcystin, nor nodularin, nor cylindrospermopsin directly interact with human toll-like receptors

HANSEN, John D., Keith A. LOFTIN, Zachary LAUGHREY a Ondřej ADAMOVSKÝ

Základní údaje

Originální název

Neither microcystin, nor nodularin, nor cylindrospermopsin directly interact with human toll-like receptors

Autoři

HANSEN, John D. (840 Spojené státy), Keith A. LOFTIN (840 Spojené státy), Zachary LAUGHREY (840 Spojené státy) a Ondřej ADAMOVSKÝ (203 Česká republika, garant, domácí)

Vydání

Chemosphere, OXFORD, PERGAMON-ELSEVIER SCIENCE LTD, 2021, 0045-6535

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10511 Environmental sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 8.943

Kód RIV

RIV/00216224:14310/21:00122023

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.chemosphere.2021.129623

UT WoS

000642944800006

Klíčová slova anglicky

Cyanotoxin; Toll-like receptor; Inflammation; NF-kappa B

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 1. 8. 2021 22:03, Mgr. Michaela Hylsová, Ph.D.

Anotace

V originále

Various stressors including temperature, environmental chemicals, and toxins can have profound impacts on immunity to pathogens. Increased eutrophication near rivers and lakes coupled with climate change are predicted to lead to increased algal blooms. Currently, the effects of cyanobacterial toxins on disease resistance in mammals is a largely unexplored area of research. Recent studies have suggested that freshwater cyanotoxins can elicit immunomodulation through interaction with specific components of innate immunity, thus potentially altering disease susceptibility parameters for fish, wildlife, and human health owing to the conserved nature of the vertebrate immune system. In this study, we investigated the effects of three microcystin congeners (LR, LA, and RR), nodularin-R, and cylin-drospermopsin for their ability to directly interact with nine different human Toll-like receptors (TLRs)-key pathogen recognition receptors for innate immunity. Toxin concentrations were verified by LC/MS/MS prior to use. Using an established HEK293-hTLR NF-kappa B reporter assay, we concluded that none of the tested toxins (29-90 nM final concentration) directly interacted with human TLRs in either an agonistic or antagonistic manner. These results suggest that earlier reports of cyanotoxin-induced NF-kappa B responses likely occur through different surface receptors to mediate inflammation. Published by Elsevier Ltd.

Návaznosti

EF17_043/0009632, projekt VaV

Název: CETOCOEN Excellence

LM2018121, projekt VaV

Název: Výzkumná infrastruktura RECETOX (Akronym: RECETOX RI)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, RECETOX RI

857560, interní kód MU
(Kód CEP: EF17_043/0009632)

Název: CETOCOEN Excellence (Akronym: CETOCOEN Excellence)

Investor: Evropská unie, CETOCOEN Excellence, Spreading excellence and widening participation

Citovat

HANSEN, John D., Keith A. LOFTIN, Zachary LAUGHREY a Ondřej ADAMOVSKÝ. Neither microcystin, nor nodularin, nor cylindrospermopsin directly interact with human toll-like receptors. Chemosphere. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD, 2021, roč. 274, July 2021, s. 1-5. ISSN 0045-6535. Dostupné z: https://dx.doi.org/10.1016/j.chemosphere.2021.129623.

@article{1784565,
   author = {Hansen, John D. and Loftin, Keith A. and Laughrey, Zachary and Adamovský, Ondřej},
   article_location = {OXFORD},
   article_number = {July 2021},
   doi = {http://dx.doi.org/10.1016/j.chemosphere.2021.129623},
   keywords = {Cyanotoxin; Toll-like receptor; Inflammation; NF-kappa B},
   language = {eng},
   issn = {0045-6535},
   journal = {Chemosphere},
   title = {Neither microcystin, nor nodularin, nor cylindrospermopsin directly interact with human toll-like receptors},
   url = {https://www.sciencedirect.com/science/article/pii/S0045653521000928?via%3Dihub},
   volume = {274},
   year = {2021}
}

TY  - JOUR
ID  - 1784565
AU  - Hansen, John D. - Loftin, Keith A. - Laughrey, Zachary - Adamovský, Ondřej
PY  - 2021
TI  - Neither microcystin, nor nodularin, nor cylindrospermopsin directly interact with human toll-like receptors
JF  - Chemosphere
VL  - 274
IS  - July 2021
SP  - 1-5
EP  - 1-5
PB  - PERGAMON-ELSEVIER SCIENCE LTD
SN  - 00456535
KW  - Cyanotoxin
KW  - Toll-like receptor
KW  - Inflammation
KW  - NF-kappa B
UR  - https://www.sciencedirect.com/science/article/pii/S0045653521000928?via%3Dihub
N2  - Various stressors including temperature, environmental chemicals, and toxins can have profound impacts on immunity to pathogens. Increased eutrophication near rivers and lakes coupled with climate change are predicted to lead to increased algal blooms. Currently, the effects of cyanobacterial toxins on disease resistance in mammals is a largely unexplored area of research. Recent studies have suggested that freshwater cyanotoxins can elicit immunomodulation through interaction with specific components of innate immunity, thus potentially altering disease susceptibility parameters for fish, wildlife, and human health owing to the conserved nature of the vertebrate immune system. In this study, we investigated the effects of three microcystin congeners (LR, LA, and RR), nodularin-R, and cylin-drospermopsin for their ability to directly interact with nine different human Toll-like receptors (TLRs)-key pathogen recognition receptors for innate immunity. Toxin concentrations were verified by LC/MS/MS prior to use. Using an established HEK293-hTLR NF-kappa B reporter assay, we concluded that none of the tested toxins (29-90 nM final concentration) directly interacted with human TLRs in either an agonistic or antagonistic manner. These results suggest that earlier reports of cyanotoxin-induced NF-kappa B responses likely occur through different surface receptors to mediate inflammation. Published by Elsevier Ltd.
ER  -

HANSEN, John D., Keith A. LOFTIN, Zachary LAUGHREY a Ondřej ADAMOVSKÝ. Neither microcystin, nor nodularin, nor cylindrospermopsin directly interact with human toll-like receptors. \textit{Chemosphere}. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD, 2021, roč.~274, July 2021, s.~1-5. ISSN~0045-6535. Dostupné z: https://dx.doi.org/10.1016/j.chemosphere.2021.129623.

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