Histopathology of laboratory-reared Nothobranchius fishes:
Mycobacterial infections versus neoplastic lesions

J 2021

Histopathology of laboratory-reared Nothobranchius fishes: Mycobacterial infections versus neoplastic lesions

DYKOVÁ, Iva, Jakub ŽÁK, Martin REICHARD, Kamila SOUČKOVÁ, Ondřej SLABÝ et. al.

Základní údaje

Originální název

Histopathology of laboratory-reared Nothobranchius fishes: Mycobacterial infections versus neoplastic lesions

Autoři

DYKOVÁ, Iva (203 Česká republika, garant, domácí), Jakub ŽÁK (203 Česká republika), Martin REICHARD (203 Česká republika, domácí), Kamila SOUČKOVÁ (203 Česká republika, domácí), Ondřej SLABÝ (203 Česká republika, domácí), Vojtěch BYSTRÝ (203 Česká republika, domácí) a Radim BLAŽEK (203 Česká republika, domácí)

Vydání

Journal of Fish Diseases, Hoboken, Wiley, 2021, 0140-7775

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10617 Marine biology, freshwater biology, limnology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.580

Kód RIV

RIV/00216224:14310/21:00119124

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1111/jfd.13378

UT WoS

000639234500001

Klíčová slova anglicky

mononuclear phagocytic system; mycobacterial infections; proliferative lesions; tumour misdiagnosis

Štítky

CF BIOIT, CF GEN, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 10. 2024 14:09, Ing. Martina Blahová

Anotace

V originále

Short-lived killifishes of the genus Nothobranchius Peters, 1868 (Cyprinodontiformes) are considered promising model organisms for biomedical research on ageing and tumorigenesis. We conducted histopathological analysis of 411 adult individuals from three Nothobranchius species to study details on spontaneous age-related neoplastic lesions. Light microscopy based on H&E and toluidine blue-stained sections revealed (a) non-proliferative liver changes with pronounced vacuolation of hepatocytes; (b) proliferation of kidney haemopoietic tissue contributing to excretory system damage; (c) proliferation of splenic mononuclear haemoblasts accompanied by reduced erythropoiesis; (d) proliferation of mononuclear cell aggregates in the liver parenchyma; and (e) rare occurrence of hepatocellular adenomas. Ziehl–Neelsen (ZN) staining revealed that the proliferative lesions are a host defence response to mycobacterial infections manifested by activation of the mononuclear phagocytic system and atypical granulomatous inflammatory reaction. 16S rRNA analysis identified three species of Mycobacterium in our samples. Our findings turn attention to lesions which mimic neoplasms by their gross appearance and question the light microscopic interpretation of lesions unless differential ZN staining is included. Beyond the limitations of our morphological approach, the intensity of mycobacterial infections is a challenging opportunity for research into the molecular-genetic background of the mononuclear phagocytic system reaction in Nothobranchius killifish.

Návaznosti

GA19-20873S, projekt VaV

Název: Klíčové mutace v nádorovém genomu Nothobranchius furzeri: od nádorové biologie k návrhu experimentálního modelu sporadické kancerogeneze

Investor: Grantová agentura ČR, Klíčové mutace v nádorovém genomu Nothobranchius furzeri: od nádorové biologie k návrhu experimentálního modelu sporadické kancerogeneze

90132, velká výzkumná infrastruktura

Název: NCMG II

Citovat

DYKOVÁ, Iva, Jakub ŽÁK, Martin REICHARD, Kamila SOUČKOVÁ, Ondřej SLABÝ, Vojtěch BYSTRÝ a Radim BLAŽEK. Histopathology of laboratory-reared Nothobranchius fishes: Mycobacterial infections versus neoplastic lesions. Journal of Fish Diseases. Hoboken: Wiley, 2021, roč. 44, č. 8, s. 1179-1190. ISSN 0140-7775. Dostupné z: https://dx.doi.org/10.1111/jfd.13378.

@article{1785678,
   author = {Dyková, Iva and Žák, Jakub and Reichard, Martin and Součková, Kamila and Slabý, Ondřej and Bystrý, Vojtěch and Blažek, Radim},
   article_location = {Hoboken},
   article_number = {8},
   doi = {http://dx.doi.org/10.1111/jfd.13378},
   keywords = {mononuclear phagocytic system; mycobacterial infections; proliferative lesions; tumour misdiagnosis},
   language = {eng},
   issn = {0140-7775},
   journal = {Journal of Fish Diseases},
   title = {Histopathology of laboratory-reared Nothobranchius fishes: Mycobacterial infections versus neoplastic lesions},
   url = {https://doi.org/10.1111/jfd.13378},
   volume = {44},
   year = {2021}
}

TY  - JOUR
ID  - 1785678
AU  - Dyková, Iva - Žák, Jakub - Reichard, Martin - Součková, Kamila - Slabý, Ondřej - Bystrý, Vojtěch - Blažek, Radim
PY  - 2021
TI  - Histopathology of laboratory-reared Nothobranchius fishes: Mycobacterial infections versus neoplastic lesions
JF  - Journal of Fish Diseases
VL  - 44
IS  - 8
SP  - 1179-1190
EP  - 1179-1190
PB  - Wiley
SN  - 01407775
KW  - mononuclear phagocytic system
KW  - mycobacterial infections
KW  - proliferative lesions
KW  - tumour misdiagnosis
UR  - https://doi.org/10.1111/jfd.13378
N2  - Short-lived killifishes of the genus Nothobranchius Peters, 1868 (Cyprinodontiformes) are considered promising model organisms for biomedical research on ageing and tumorigenesis. We conducted histopathological analysis of 411 adult individuals from three Nothobranchius species to study details on spontaneous age-related neoplastic lesions. Light microscopy based on H&E and toluidine blue-stained sections revealed (a) non-proliferative liver changes with pronounced vacuolation of hepatocytes; (b) proliferation of kidney haemopoietic tissue contributing to excretory system damage; (c) proliferation of splenic mononuclear haemoblasts accompanied by reduced erythropoiesis; (d) proliferation of mononuclear cell aggregates in the liver parenchyma; and (e) rare occurrence of hepatocellular adenomas. Ziehl–Neelsen (ZN) staining revealed that the proliferative lesions are a host defence response to mycobacterial infections manifested by activation of the mononuclear phagocytic system and atypical granulomatous inflammatory reaction. 16S rRNA analysis identified three species of Mycobacterium in our samples. Our findings turn attention to lesions which mimic neoplasms by their gross appearance and question the light microscopic interpretation of lesions unless differential ZN staining is included. Beyond the limitations of our morphological approach, the intensity of mycobacterial infections is a challenging opportunity for research into the molecular-genetic background of the mononuclear phagocytic system reaction in Nothobranchius killifish.
ER  -

DYKOVÁ, Iva, Jakub ŽÁK, Martin REICHARD, Kamila SOUČKOVÁ, Ondřej SLABÝ, Vojtěch BYSTRÝ a Radim BLAŽEK. Histopathology of laboratory-reared Nothobranchius fishes: Mycobacterial infections versus neoplastic lesions. \textit{Journal of Fish Diseases}. Hoboken: Wiley, 2021, roč.~44, č.~8, s.~1179-1190. ISSN~0140-7775. Dostupné z: https://dx.doi.org/10.1111/jfd.13378.

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