DYKOVÁ, Iva, Jakub ŽÁK, Martin REICHARD, Kamila SOUČKOVÁ, Ondřej SLABÝ, Vojtěch BYSTRÝ a Radim BLAŽEK. Histopathology of laboratory-reared Nothobranchius fishes: Mycobacterial infections versus neoplastic lesions. Journal of Fish Diseases. Hoboken: Wiley, 2021, roč. 44, č. 8, s. 1179-1190. ISSN 0140-7775. Dostupné z: https://dx.doi.org/10.1111/jfd.13378. |
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@article{1785678, author = {Dyková, Iva and Žák, Jakub and Reichard, Martin and Součková, Kamila and Slabý, Ondřej and Bystrý, Vojtěch and Blažek, Radim}, article_location = {Hoboken}, article_number = {8}, doi = {http://dx.doi.org/10.1111/jfd.13378}, keywords = {mononuclear phagocytic system; mycobacterial infections; proliferative lesions; tumour misdiagnosis}, language = {eng}, issn = {0140-7775}, journal = {Journal of Fish Diseases}, title = {Histopathology of laboratory-reared Nothobranchius fishes: Mycobacterial infections versus neoplastic lesions}, url = {https://doi.org/10.1111/jfd.13378}, volume = {44}, year = {2021} }
TY - JOUR ID - 1785678 AU - Dyková, Iva - Žák, Jakub - Reichard, Martin - Součková, Kamila - Slabý, Ondřej - Bystrý, Vojtěch - Blažek, Radim PY - 2021 TI - Histopathology of laboratory-reared Nothobranchius fishes: Mycobacterial infections versus neoplastic lesions JF - Journal of Fish Diseases VL - 44 IS - 8 SP - 1179-1190 EP - 1179-1190 PB - Wiley SN - 01407775 KW - mononuclear phagocytic system KW - mycobacterial infections KW - proliferative lesions KW - tumour misdiagnosis UR - https://doi.org/10.1111/jfd.13378 N2 - Short-lived killifishes of the genus Nothobranchius Peters, 1868 (Cyprinodontiformes) are considered promising model organisms for biomedical research on ageing and tumorigenesis. We conducted histopathological analysis of 411 adult individuals from three Nothobranchius species to study details on spontaneous age-related neoplastic lesions. Light microscopy based on H&E and toluidine blue-stained sections revealed (a) non-proliferative liver changes with pronounced vacuolation of hepatocytes; (b) proliferation of kidney haemopoietic tissue contributing to excretory system damage; (c) proliferation of splenic mononuclear haemoblasts accompanied by reduced erythropoiesis; (d) proliferation of mononuclear cell aggregates in the liver parenchyma; and (e) rare occurrence of hepatocellular adenomas. Ziehl–Neelsen (ZN) staining revealed that the proliferative lesions are a host defence response to mycobacterial infections manifested by activation of the mononuclear phagocytic system and atypical granulomatous inflammatory reaction. 16S rRNA analysis identified three species of Mycobacterium in our samples. Our findings turn attention to lesions which mimic neoplasms by their gross appearance and question the light microscopic interpretation of lesions unless differential ZN staining is included. Beyond the limitations of our morphological approach, the intensity of mycobacterial infections is a challenging opportunity for research into the molecular-genetic background of the mononuclear phagocytic system reaction in Nothobranchius killifish. ER -
DYKOVÁ, Iva, Jakub ŽÁK, Martin REICHARD, Kamila SOUČKOVÁ, Ondřej SLABÝ, Vojtěch BYSTRÝ a Radim BLAŽEK. Histopathology of laboratory-reared Nothobranchius fishes: Mycobacterial infections versus neoplastic lesions. \textit{Journal of Fish Diseases}. Hoboken: Wiley, 2021, roč.~44, č.~8, s.~1179-1190. ISSN~0140-7775. Dostupné z: https://dx.doi.org/10.1111/jfd.13378.
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