2021
Atomic force microscopy and surface plasmon resonance for real-time single-cell monitoring of bacteriophage-mediated lysis of bacteria
OBOŘILOVÁ, Radka, Hana ŠIMEČKOVÁ, Matěj PASTUCHA, Šimon KLIMOVIČ, Ivana VÍŠOVÁ et. al.Základní údaje
Originální název
Atomic force microscopy and surface plasmon resonance for real-time single-cell monitoring of bacteriophage-mediated lysis of bacteria
Autoři
OBOŘILOVÁ, Radka (203 Česká republika, domácí), Hana ŠIMEČKOVÁ (203 Česká republika, domácí), Matěj PASTUCHA (203 Česká republika, domácí), Šimon KLIMOVIČ (203 Česká republika, domácí), Ivana VÍŠOVÁ (203 Česká republika), Jan PŘIBYL (203 Česká republika, domácí), Hana VAISOCHEROVÁ-LÍSALOVÁ (203 Česká republika), Roman PANTŮČEK (203 Česká republika, domácí), Petr SKLÁDAL (203 Česká republika, domácí), Ivana MAŠLAŇOVÁ (203 Česká republika, domácí) a Zdeněk FARKA (203 Česká republika, garant, domácí)
Vydání
Nanoscale, Cambridge, Royal Society of Chemistry, 2021, 2040-3364
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
10608 Biochemistry and molecular biology
Stát vydavatele
Velká Británie a Severní Irsko
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Impakt faktor
Impact factor: 8.307
Kód RIV
RIV/00216224:14310/21:00119126
Organizační jednotka
Přírodovědecká fakulta
UT WoS
000680101900001
Klíčová slova anglicky
Atomic force microscopy; AFM; Surface plasmon resonance; SPR; Staphylococcus aureus; Lysis; Lysostaphin; Bacteriophage; Phage therapy
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 2. 11. 2024 20:05, Mgr. Adéla Pešková
Anotace
V originále
The growing incidence of multidrug-resistant bacterial strains presents a major challenge in modern medicine. Antibiotic resistance is often exhibited by Staphylococcus aureus, which causes severe infections in human and animal hosts and leads to significant economic losses. Antimicrobial agents with enzymatic activity (enzybiotics) and phage therapy represent promising and effective alternatives to classic antibiotics. However, new tools are needed to study phage–bacteria interactions and bacterial lysis with high resolution and in real-time. Here, we introduce a method for studying the lysis of S. aureus at the single-cell level in real-time using atomic force microscopy (AFM) in liquid. We demonstrate the ability of the method to monitor the effect of the enzyme lysostaphin on S. aureus and the lytic action of the Podoviridae phage P68. AFM allowed the topographic and biomechanical properties of individual bacterial cells to be monitored at high resolution over the course of their lysis, under near-physiological conditions. Changes in the stiffness of S. aureus cells during lysis were studied by analyzing force–distance curves to determine Young's modulus. This allowed observing a progressive decline in cellular stiffness corresponding to the disintegration of the cell envelope. The AFM experiments were complemented by surface plasmon resonance (SPR) experiments that provided information on the kinetics of phage-bacterium binding and the subsequent lytic processes. This approach forms the foundation of an innovative framework for studying the lysis of individual bacteria that may facilitate the further development of phage therapy.
Návaznosti
| GA18-13064S, projekt VaV |
| ||
| GA21-03156S, projekt VaV |
| ||
| LM2018127, projekt VaV |
| ||
| LQ1601, projekt VaV |
| ||
| LTAB19011, projekt VaV |
|