Atomic force microscopy and surface plasmon resonance for
real-time single-cell monitoring of bacteriophage-mediated
lysis of bacteria

J 2021

Atomic force microscopy and surface plasmon resonance for real-time single-cell monitoring of bacteriophage-mediated lysis of bacteria

OBOŘILOVÁ, Radka, Hana ŠIMEČKOVÁ, Matěj PASTUCHA, Šimon KLIMOVIČ, Ivana VÍŠOVÁ et. al.

Basic information

Original name

Atomic force microscopy and surface plasmon resonance for real-time single-cell monitoring of bacteriophage-mediated lysis of bacteria

Authors

OBOŘILOVÁ, Radka (203 Czech Republic, belonging to the institution), Hana ŠIMEČKOVÁ (203 Czech Republic, belonging to the institution), Matěj PASTUCHA (203 Czech Republic, belonging to the institution), Šimon KLIMOVIČ (203 Czech Republic, belonging to the institution), Ivana VÍŠOVÁ (203 Czech Republic), Jan PŘIBYL (203 Czech Republic, belonging to the institution), Hana VAISOCHEROVÁ-LÍSALOVÁ (203 Czech Republic), Roman PANTŮČEK (203 Czech Republic, belonging to the institution), Petr SKLÁDAL (203 Czech Republic, belonging to the institution), Ivana MAŠLAŇOVÁ (203 Czech Republic, belonging to the institution) and Zdeněk FARKA (203 Czech Republic, guarantor, belonging to the institution)

Edition

Nanoscale, Cambridge, Royal Society of Chemistry, 2021, 2040-3364

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 8.307

RIV identification code

RIV/00216224:14310/21:00119126

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1039/D1NR02921E

UT WoS

000680101900001

Keywords in English

Atomic force microscopy; AFM; Surface plasmon resonance; SPR; Staphylococcus aureus; Lysis; Lysostaphin; Bacteriophage; Phage therapy

Abstract

V originále

The growing incidence of multidrug-resistant bacterial strains presents a major challenge in modern medicine. Antibiotic resistance is often exhibited by Staphylococcus aureus, which causes severe infections in human and animal hosts and leads to significant economic losses. Antimicrobial agents with enzymatic activity (enzybiotics) and phage therapy represent promising and effective alternatives to classic antibiotics. However, new tools are needed to study phage–bacteria interactions and bacterial lysis with high resolution and in real-time. Here, we introduce a method for studying the lysis of S. aureus at the single-cell level in real-time using atomic force microscopy (AFM) in liquid. We demonstrate the ability of the method to monitor the effect of the enzyme lysostaphin on S. aureus and the lytic action of the Podoviridae phage P68. AFM allowed the topographic and biomechanical properties of individual bacterial cells to be monitored at high resolution over the course of their lysis, under near-physiological conditions. Changes in the stiffness of S. aureus cells during lysis were studied by analyzing force–distance curves to determine Young's modulus. This allowed observing a progressive decline in cellular stiffness corresponding to the disintegration of the cell envelope. The AFM experiments were complemented by surface plasmon resonance (SPR) experiments that provided information on the kinetics of phage-bacterium binding and the subsequent lytic processes. This approach forms the foundation of an innovative framework for studying the lysis of individual bacteria that may facilitate the further development of phage therapy.

Links

GA18-13064S, research and development project

Name: Analýza interakcí mezi polyvalentním terapeutickým fágem druhu Twort-like a jeho hostitelem Staphylococcus aureus

Investor: Czech Science Foundation

GA21-03156S, research and development project

Name: Foton-upkonverzní značky pro mikrofluidní jednomolekulové imunostanovení proteinových biomarkerů

Investor: Czech Science Foundation

LM2018127, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

LTAB19011, research and development project

Name: Luminiscenční imunostanovení jako citlivý nástroj pro diagnostiku nemocí včel

Investor: Ministry of Education, Youth and Sports of the CR, Sensitive bioanalytical tools for the surveillance of honey bee diseases, INTER-ACTION

90127, large research infrastructures

Name: CIISB II

Citovat

OBOŘILOVÁ, Radka, Hana ŠIMEČKOVÁ, Matěj PASTUCHA, Šimon KLIMOVIČ, Ivana VÍŠOVÁ, Jan PŘIBYL, Hana VAISOCHEROVÁ-LÍSALOVÁ, Roman PANTŮČEK, Petr SKLÁDAL, Ivana MAŠLAŇOVÁ and Zdeněk FARKA. Atomic force microscopy and surface plasmon resonance for real-time single-cell monitoring of bacteriophage-mediated lysis of bacteria. Nanoscale. Cambridge: Royal Society of Chemistry, 2021, vol. 13, No 31, p. 13538-13549. ISSN 2040-3364. Available from: https://dx.doi.org/10.1039/D1NR02921E.

@article{1785779,
   author = {Obořilová, Radka and Šimečková, Hana and Pastucha, Matěj and Klimovič, Šimon and Víšová, Ivana and Přibyl, Jan and VaisocherováandLísalová, Hana and Pantůček, Roman and Skládal, Petr and Mašlaňová, Ivana and Farka, Zdeněk},
   article_location = {Cambridge},
   article_number = {31},
   doi = {http://dx.doi.org/10.1039/D1NR02921E},
   keywords = {Atomic force microscopy; AFM; Surface plasmon resonance; SPR; Staphylococcus aureus; Lysis; Lysostaphin; Bacteriophage; Phage therapy},
   language = {eng},
   issn = {2040-3364},
   journal = {Nanoscale},
   title = {Atomic force microscopy and surface plasmon resonance for real-time single-cell monitoring of bacteriophage-mediated lysis of bacteria},
   url = {https://pubs.rsc.org/en/content/articlelanding/2021/NR/D1NR02921E},
   volume = {13},
   year = {2021}
}

TY  - JOUR
ID  - 1785779
AU  - Obořilová, Radka - Šimečková, Hana - Pastucha, Matěj - Klimovič, Šimon - Víšová, Ivana - Přibyl, Jan - Vaisocherová-Lísalová, Hana - Pantůček, Roman - Skládal, Petr - Mašlaňová, Ivana - Farka, Zdeněk
PY  - 2021
TI  - Atomic force microscopy and surface plasmon resonance for real-time single-cell monitoring of bacteriophage-mediated lysis of bacteria
JF  - Nanoscale
VL  - 13
IS  - 31
SP  - 13538-13549
EP  - 13538-13549
PB  - Royal Society of Chemistry
SN  - 20403364
KW  - Atomic force microscopy
KW  - AFM
KW  - Surface plasmon resonance
KW  - SPR
KW  - Staphylococcus aureus
KW  - Lysis
KW  - Lysostaphin
KW  - Bacteriophage
KW  - Phage therapy
UR  - https://pubs.rsc.org/en/content/articlelanding/2021/NR/D1NR02921E
N2  - The growing incidence of multidrug-resistant bacterial strains presents a major challenge in modern medicine. Antibiotic resistance is often exhibited by Staphylococcus aureus, which causes severe infections in human and animal hosts and leads to significant economic losses. Antimicrobial agents with enzymatic activity (enzybiotics) and phage therapy represent promising and effective alternatives to classic antibiotics. However, new tools are needed to study phage–bacteria interactions and bacterial lysis with high resolution and in real-time. Here, we introduce a method for studying the lysis of S. aureus at the single-cell level in real-time using atomic force microscopy (AFM) in liquid. We demonstrate the ability of the method to monitor the effect of the enzyme lysostaphin on S. aureus and the lytic action of the Podoviridae phage P68. AFM allowed the topographic and biomechanical properties of individual bacterial cells to be monitored at high resolution over the course of their lysis, under near-physiological conditions. Changes in the stiffness of S. aureus cells during lysis were studied by analyzing force–distance curves to determine Young's modulus. This allowed observing a progressive decline in cellular stiffness corresponding to the disintegration of the cell envelope. The AFM experiments were complemented by surface plasmon resonance (SPR) experiments that provided information on the kinetics of phage-bacterium binding and the subsequent lytic processes. This approach forms the foundation of an innovative framework for studying the lysis of individual bacteria that may facilitate the further development of phage therapy.
ER  -

OBOŘILOVÁ, Radka, Hana ŠIMEČKOVÁ, Matěj PASTUCHA, Šimon KLIMOVIČ, Ivana VÍŠOVÁ, Jan PŘIBYL, Hana VAISOCHEROVÁ-LÍSALOVÁ, Roman PANTŮČEK, Petr SKLÁDAL, Ivana MAŠLAŇOVÁ and Zdeněk FARKA. Atomic force microscopy and surface plasmon resonance for real-time single-cell monitoring of bacteriophage-mediated lysis of bacteria. \textit{Nanoscale}. Cambridge: Royal Society of Chemistry, 2021, vol.~13, No~31, p.~13538-13549. ISSN~2040-3364. Available from: https://dx.doi.org/10.1039/D1NR02921E.

Detailed Information on Publication Record