Comparison of imidacloprid, propiconazole, and
nanopropiconazole effects on the development, behavior, and
gene expression biomarkers of the Pacific oyster (Magallana
gigas)

J 2021

Comparison of imidacloprid, propiconazole, and nanopropiconazole effects on the development, behavior, and gene expression biomarkers of the Pacific oyster (Magallana gigas)

KUCHOVSKÁ, Eliška, Bénédicte MORIN, María del Rocío LÓPEZ CABEZA, Mathilde BARRÉ, Corentin GOUFFIER et. al.

Základní údaje

Originální název

Comparison of imidacloprid, propiconazole, and nanopropiconazole effects on the development, behavior, and gene expression biomarkers of the Pacific oyster (Magallana gigas)

Autoři

KUCHOVSKÁ, Eliška (203 Česká republika, domácí), Bénédicte MORIN (250 Francie), María del Rocío LÓPEZ CABEZA (724 Španělsko, domácí), Mathilde BARRÉ (250 Francie), Corentin GOUFFIER (250 Francie), Lucie BLÁHOVÁ (203 Česká republika, domácí), Jérôme CACHOT (250 Francie), Luděk BLÁHA (203 Česká republika, garant, domácí) a Patrice GONZALEZ (250 Francie)

Vydání

Science of the Total Environment, Amsterdam, Elsevier Science, 2021, 0048-9697

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10511 Environmental sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 10.753

Kód RIV

RIV/00216224:14310/21:00119130

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.scitotenv.2020.142921

UT WoS

000614249600100

Klíčová slova anglicky

Embryotoxicity; Gene expression; Pacific oyster; Pesticide; Sublethal effect; Swimming behavior

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 1. 2022 11:09, Mgr. Marie Šípková, DiS.

Anotace

V originále

Coastal areas are final recipients of various contaminants including pesticides. The effects of pesticides on non-target organisms are often unclear, especially at environmentally relevant concentrations. This study investigated the impacts of insecticide imidacloprid (IMI) and fungicide propiconazole (PRO), some of the most detected pesticides in the Arcachon Bay in France. This work also included the research of propiconazole nanoformulation (nanoPRO). The effects were assessed studying the development of the early life stages of the Pacific oyster (Magallana gigas). Oyster embryos were exposed for 24, 30, and 42 h (depending on the endpoint) at 24 °C to environmentally relevant concentrations of the two pesticides as well as to nanoPRO. The research focused on sublethal endpoints such as the presence of developmental malformations, alterations of locomotion patterns, or changes in the gene expression levels. No developmental abnormalities were observed after exposure to environmental concentrations detected in the Arcachon Bay in recent years (maximal detected concentration of IMI and PRO were 174 ng/L and 29 ng/L, respectively). EC50 of PRO and nanoPRO were comparable, 2.93 ± 1.35 and 2.26 ± 1.36 mg/L, while EC50 of IMI exceeded 200 mg/L. IMI did not affect larval behavior. PRO affected larval movement trajectory and decreased average larvae swimming speed (2 μg/L), while nanoPRO increased the maximal larvae swimming speed (0.02 μg/L). PRO upregulated especially genes linked to reactive oxygen species (ROS) production and detoxification. NanoPRO effects on gene expression were less pronounced - half of the genes were altered in comparison with PRO. IMI induced a strong dose-response impact on the genes linked to the detoxification, ROS production, cell cycle, and apoptosis regulation. In conclusion, our results suggest that current pesticide concentrations detected in the Arcachon Bay are safe for the Pacific oyster early development, but they might have a small direct effect via altered gene expressions, whose longer-term impacts cannot be ruled out.

Návaznosti

GA18-19324S, projekt VaV

Název: Nanopesticidy v životním prostředí – porozumění jejich osudu, biodostupnosti, účinkům a rizikům v půdě a vodě

Investor: Grantová agentura ČR, Nanopesticides in the environment – understanding their fate, bioavailability, effects and risks in soil and water

LM2018121, projekt VaV

Název: Výzkumná infrastruktura RECETOX (Akronym: RECETOX RI)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, RECETOX RI

Citovat

KUCHOVSKÁ, Eliška, Bénédicte MORIN, María del Rocío LÓPEZ CABEZA, Mathilde BARRÉ, Corentin GOUFFIER, Lucie BLÁHOVÁ, Jérôme CACHOT, Luděk BLÁHA a Patrice GONZALEZ. Comparison of imidacloprid, propiconazole, and nanopropiconazole effects on the development, behavior, and gene expression biomarkers of the Pacific oyster (Magallana gigas). Science of the Total Environment. Amsterdam: Elsevier Science, 2021, roč. 764, April 2021, s. 1-11. ISSN 0048-9697. Dostupné z: https://dx.doi.org/10.1016/j.scitotenv.2020.142921.

@article{1786145,
   author = {Kuchovská, Eliška and Morin, Bénédicte and López Cabeza, María del Rocío and Barré, Mathilde and Gouffier, Corentin and Bláhová, Lucie and Cachot, Jérôme and Bláha, Luděk and Gonzalez, Patrice},
   article_location = {Amsterdam},
   article_number = {April 2021},
   doi = {http://dx.doi.org/10.1016/j.scitotenv.2020.142921},
   keywords = {Embryotoxicity; Gene expression; Pacific oyster; Pesticide; Sublethal effect; Swimming behavior},
   language = {eng},
   issn = {0048-9697},
   journal = {Science of the Total Environment},
   title = {Comparison of imidacloprid, propiconazole, and nanopropiconazole effects on the development, behavior, and gene expression biomarkers of the Pacific oyster (Magallana gigas)},
   url = {https://www.sciencedirect.com/science/article/pii/S0048969720364512?via%3Dihub},
   volume = {764},
   year = {2021}
}

TY  - JOUR
ID  - 1786145
AU  - Kuchovská, Eliška - Morin, Bénédicte - López Cabeza, María del Rocío - Barré, Mathilde - Gouffier, Corentin - Bláhová, Lucie - Cachot, Jérôme - Bláha, Luděk - Gonzalez, Patrice
PY  - 2021
TI  - Comparison of imidacloprid, propiconazole, and nanopropiconazole effects on the development, behavior, and gene expression biomarkers of the Pacific oyster (Magallana gigas)
JF  - Science of the Total Environment
VL  - 764
IS  - April 2021
SP  - 1-11
EP  - 1-11
PB  - Elsevier Science
SN  - 00489697
KW  - Embryotoxicity
KW  - Gene expression
KW  - Pacific oyster
KW  - Pesticide
KW  - Sublethal effect
KW  - Swimming behavior
UR  - https://www.sciencedirect.com/science/article/pii/S0048969720364512?via%3Dihub
N2  - Coastal areas are final recipients of various contaminants including pesticides. The effects of pesticides on non-target organisms are often unclear, especially at environmentally relevant concentrations. This study investigated the impacts of insecticide imidacloprid (IMI) and fungicide propiconazole (PRO), some of the most detected pesticides in the Arcachon Bay in France. This work also included the research of propiconazole nanoformulation (nanoPRO). The effects were assessed studying the development of the early life stages of the Pacific oyster (Magallana gigas). Oyster embryos were exposed for 24, 30, and 42 h (depending on the endpoint) at 24 °C to environmentally relevant concentrations of the two pesticides as well as to nanoPRO. The research focused on sublethal endpoints such as the presence of developmental malformations, alterations of locomotion patterns, or changes in the gene expression levels. No developmental abnormalities were observed after exposure to environmental concentrations detected in the Arcachon Bay in recent years (maximal detected concentration of IMI and PRO were 174 ng/L and 29 ng/L, respectively). EC50 of PRO and nanoPRO were comparable, 2.93 ± 1.35 and 2.26 ± 1.36 mg/L, while EC50 of IMI exceeded 200 mg/L. IMI did not affect larval behavior. PRO affected larval movement trajectory and decreased average larvae swimming speed (2 μg/L), while nanoPRO increased the maximal larvae swimming speed (0.02 μg/L). PRO upregulated especially genes linked to reactive oxygen species (ROS) production and detoxification. NanoPRO effects on gene expression were less pronounced - half of the genes were altered in comparison with PRO. IMI induced a strong dose-response impact on the genes linked to the detoxification, ROS production, cell cycle, and apoptosis regulation. In conclusion, our results suggest that current pesticide concentrations detected in the Arcachon Bay are safe for the Pacific oyster early development, but they might have a small direct effect via altered gene expressions, whose longer-term impacts cannot be ruled out.
ER  -

KUCHOVSKÁ, Eliška, Bénédicte MORIN, María del Rocío LÓPEZ CABEZA, Mathilde BARRÉ, Corentin GOUFFIER, Lucie BLÁHOVÁ, Jérôme CACHOT, Luděk BLÁHA a Patrice GONZALEZ. Comparison of imidacloprid, propiconazole, and nanopropiconazole effects on the development, behavior, and gene expression biomarkers of the Pacific oyster (Magallana gigas). \textit{Science of the Total Environment}. Amsterdam: Elsevier Science, 2021, roč.~764, April 2021, s.~1-11. ISSN~0048-9697. Dostupné z: https://dx.doi.org/10.1016/j.scitotenv.2020.142921.

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