Estrogenicity of chemical mixtures revealed by a panel of
bioassays

J 2021

Estrogenicity of chemical mixtures revealed by a panel of bioassays

GOMEZ, Livia, Magdalena NIEGOWSKA, Anna NAVARRO, Luca AMENDOLA, Augustine ARUKWE et. al.

Základní údaje

Originální název

Estrogenicity of chemical mixtures revealed by a panel of bioassays

Autoři

GOMEZ, Livia (380 Itálie), Magdalena NIEGOWSKA (380 Itálie), Anna NAVARRO (380 Itálie), Luca AMENDOLA (380 Itálie), Augustine ARUKWE (578 Norsko), Selim AIT-AISSA (250 Francie), Stefania BALZAMO (380 Itálie), Salvatore BARRECA (380 Itálie), Shimshon BELKIN (376 Izrael), Michal BITTNER (203 Česká republika, domácí), Luděk BLÁHA (203 Česká republika, garant, domácí), Sebastian BUCHINGER (276 Německo), Maddalena BUSETTO (380 Itálie), Mario CARERE (380 Itálie), Luisa COLZANI (380 Itálie), Pierluisa DELLAVEDOVA (380 Itálie), Nancy DENSLOW (840 Spojené státy), Beate, I. ESCHER (276 Německo), Christer HOGSTRAND (826 Velká Británie a Severní Irsko), Essa Ahsan KHAN (578 Norsko), Maria KONIG (276 Německo), Kevin J. KROLL (840 Spojené státy), Ines LACCHETTI (380 Itálie), Emmanuelle MAILLOT-MARECHAL (250 Francie), Liat MOSCOVICI (376 Izrael), Monica POTALIVO (380 Itálie), Isabella SANSEVERINO (380 Itálie), Ricardo SANTOS (620 Portugalsko), Andrea SCHIFFERLI (756 Švýcarsko), Rita SCHLICHTING (276 Německo), Susanna SFORZINI (380 Itálie), Eszter SIMON (756 Švýcarsko), Etai SHPIGEL (376 Izrael), Stephen STURZENBAUM (826 Velká Británie a Severní Irsko), Etienne VERMEIRSSEN (756 Švýcarsko), Aldo VIARENGO (380 Itálie), Inge WERNER (756 Švýcarsko) a Teresa LETTIERI (380 Itálie)

Vydání

Science of the Total Environment, Amsterdam, Elsevier Science, 2021, 0048-9697

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10511 Environmental sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 10.753

Kód RIV

RIV/00216224:14310/21:00122096

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.scitotenv.2021.147284

UT WoS

000659457100009

Klíčová slova anglicky

Bioassay; Chemical mixture; Environmental quality standard (EQS); Endocrine disrupting compound (EDC); Estrogenicity; Hormone mixture

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 14. 8. 2021 21:49, Mgr. Michaela Hylsová, Ph.D.

Anotace

V originále

Estrogenic compounds are widely released to surface waters and may cause adverse effects to sensitive aquatic species. Three hormones, estrone, 1711-estradiol and 17 alpha-ethinylestradiol, are of particular concern as they are bioactive at very low concentrations. Current analytical methods are not all sensitive enough for monitoring these substances in water and do not cover mixture effects. Bioassays could complement chemical analysis since they detect the overall effect of complex mixtures. Here, four chemical mixtures and two hormone mixtures were prepared and tested as reference materials together with two environmental water samples by eight laboratories employing nine in vitro and in vivo bioassays covering different steps involved in the estrogenic response. The reference materials included priority substances under the European Water Framework Directive, hormones and other emerging pollutants. Each substance in the mixture was present at its proposed safety limit concentration (EQS) in the European legislation. The in vitro bioassays detected the estrogenic effect of chemical mixtures even when 1711-estradiol was not present but differences in responsiveness were observed. LiBERA was the most responsive, followed by LYES. The additive effect of the hormones was captured by ER alpha-CALUX, MELN, LYES and LiBERA. Particularly, all in vitro bioassays detected the estrogenic effects in environmental water samples (EEQ values in the range of 0.75-304 & times; EQS), although the concentrations of hormones were below the limit of quantification in analytical measurements. The present study confirms the applicability of reference materials for estrogenic effects' detection through bioassays and indicates possible methodological drawbacks of some of them that may lead to false negative/positive outcomes. The observed difference in responsiveness among bioassays-based on mixture composition -is probably due to biological differences between them, suggesting that panels of bioassays with different characteristics should be applied according to specific environmental pollution conditions.

Návaznosti

LM2018121, projekt VaV

Název: Výzkumná infrastruktura RECETOX (Akronym: RECETOX RI)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, RECETOX RI

Citovat

GOMEZ, Livia, Magdalena NIEGOWSKA, Anna NAVARRO, Luca AMENDOLA, Augustine ARUKWE, Selim AIT-AISSA, Stefania BALZAMO, Salvatore BARRECA, Shimshon BELKIN, Michal BITTNER, Luděk BLÁHA, Sebastian BUCHINGER, Maddalena BUSETTO, Mario CARERE, Luisa COLZANI, Pierluisa DELLAVEDOVA, Nancy DENSLOW, Beate, I. ESCHER, Christer HOGSTRAND, Essa Ahsan KHAN, Maria KONIG, Kevin J. KROLL, Ines LACCHETTI, Emmanuelle MAILLOT-MARECHAL, Liat MOSCOVICI, Monica POTALIVO, Isabella SANSEVERINO, Ricardo SANTOS, Andrea SCHIFFERLI, Rita SCHLICHTING, Susanna SFORZINI, Eszter SIMON, Etai SHPIGEL, Stephen STURZENBAUM, Etienne VERMEIRSSEN, Aldo VIARENGO, Inge WERNER a Teresa LETTIERI. Estrogenicity of chemical mixtures revealed by a panel of bioassays. Science of the Total Environment. Amsterdam: Elsevier Science, 2021, roč. 785, September 2021, s. 1-16. ISSN 0048-9697. Dostupné z: https://dx.doi.org/10.1016/j.scitotenv.2021.147284.

@article{1786149,
   author = {Gomez, Livia and Niegowska, Magdalena and Navarro, Anna and Amendola, Luca and Arukwe, Augustine and AitandAissa, Selim and Balzamo, Stefania and Barreca, Salvatore and Belkin, Shimshon and Bittner, Michal and Bláha, Luděk and Buchinger, Sebastian and Busetto, Maddalena and Carere, Mario and Colzani, Luisa and Dellavedova, Pierluisa and Denslow, Nancy and Escher, Beate, I. and Hogstrand, Christer and Khan, Essa Ahsan and Konig, Maria and Kroll, Kevin J. and Lacchetti, Ines and MaillotandMarechal, Emmanuelle and Moscovici, Liat and Potalivo, Monica and Sanseverino, Isabella and Santos, Ricardo and Schifferli, Andrea and Schlichting, Rita and Sforzini, Susanna and Simon, Eszter and Shpigel, Etai and Sturzenbaum, Stephen and Vermeirssen, Etienne and Viarengo, Aldo and Werner, Inge and Lettieri, Teresa},
   article_location = {Amsterdam},
   article_number = {September 2021},
   doi = {http://dx.doi.org/10.1016/j.scitotenv.2021.147284},
   keywords = {Bioassay; Chemical mixture; Environmental quality standard (EQS); Endocrine disrupting compound (EDC); Estrogenicity; Hormone mixture},
   language = {eng},
   issn = {0048-9697},
   journal = {Science of the Total Environment},
   title = {Estrogenicity of chemical mixtures revealed by a panel of bioassays},
   url = {https://www.sciencedirect.com/science/article/pii/S004896972102355X?via%3Dihub},
   volume = {785},
   year = {2021}
}

TY  - JOUR
ID  - 1786149
AU  - Gomez, Livia - Niegowska, Magdalena - Navarro, Anna - Amendola, Luca - Arukwe, Augustine - Ait-Aissa, Selim - Balzamo, Stefania - Barreca, Salvatore - Belkin, Shimshon - Bittner, Michal - Bláha, Luděk - Buchinger, Sebastian - Busetto, Maddalena - Carere, Mario - Colzani, Luisa - Dellavedova, Pierluisa - Denslow, Nancy - Escher, Beate, I. - Hogstrand, Christer - Khan, Essa Ahsan - Konig, Maria - Kroll, Kevin J. - Lacchetti, Ines - Maillot-Marechal, Emmanuelle - Moscovici, Liat - Potalivo, Monica - Sanseverino, Isabella - Santos, Ricardo - Schifferli, Andrea - Schlichting, Rita - Sforzini, Susanna - Simon, Eszter - Shpigel, Etai - Sturzenbaum, Stephen - Vermeirssen, Etienne - Viarengo, Aldo - Werner, Inge - Lettieri, Teresa
PY  - 2021
TI  - Estrogenicity of chemical mixtures revealed by a panel of bioassays
JF  - Science of the Total Environment
VL  - 785
IS  - September 2021
SP  - 1-16
EP  - 1-16
PB  - Elsevier Science
SN  - 00489697
KW  - Bioassay
KW  - Chemical mixture
KW  - Environmental quality standard (EQS)
KW  - Endocrine disrupting compound (EDC)
KW  - Estrogenicity
KW  - Hormone mixture
UR  - https://www.sciencedirect.com/science/article/pii/S004896972102355X?via%3Dihub
N2  - Estrogenic compounds are widely released to surface waters and may cause adverse effects to sensitive aquatic species. Three hormones, estrone, 1711-estradiol and 17 alpha-ethinylestradiol, are of particular concern as they are bioactive at very low concentrations. Current analytical methods are not all sensitive enough for monitoring these substances in water and do not cover mixture effects. Bioassays could complement chemical analysis since they detect the overall effect of complex mixtures. Here, four chemical mixtures and two hormone mixtures were prepared and tested as reference materials together with two environmental water samples by eight laboratories employing nine in vitro and in vivo bioassays covering different steps involved in the estrogenic response. The reference materials included priority substances under the European Water Framework Directive, hormones and other emerging pollutants. Each substance in the mixture was present at its proposed safety limit concentration (EQS) in the European legislation. The in vitro bioassays detected the estrogenic effect of chemical mixtures even when 1711-estradiol was not present but differences in responsiveness were observed. LiBERA was the most responsive, followed by LYES. The additive effect of the hormones was captured by ER alpha-CALUX, MELN, LYES and LiBERA. Particularly, all in vitro bioassays detected the estrogenic effects in environmental water samples (EEQ values in the range of 0.75-304 & times; EQS), although the concentrations of hormones were below the limit of quantification in analytical measurements. The present study confirms the applicability of reference materials for estrogenic effects' detection through bioassays and indicates possible methodological drawbacks of some of them that may lead to false negative/positive outcomes. The observed difference in responsiveness among bioassays-based on mixture composition -is probably due to biological differences between them, suggesting that panels of bioassays with different characteristics should be applied according to specific environmental pollution conditions.
ER  -

GOMEZ, Livia, Magdalena NIEGOWSKA, Anna NAVARRO, Luca AMENDOLA, Augustine ARUKWE, Selim AIT-AISSA, Stefania BALZAMO, Salvatore BARRECA, Shimshon BELKIN, Michal BITTNER, Luděk BLÁHA, Sebastian BUCHINGER, Maddalena BUSETTO, Mario CARERE, Luisa COLZANI, Pierluisa DELLAVEDOVA, Nancy DENSLOW, Beate, I. ESCHER, Christer HOGSTRAND, Essa Ahsan KHAN, Maria KONIG, Kevin J. KROLL, Ines LACCHETTI, Emmanuelle MAILLOT-MARECHAL, Liat MOSCOVICI, Monica POTALIVO, Isabella SANSEVERINO, Ricardo SANTOS, Andrea SCHIFFERLI, Rita SCHLICHTING, Susanna SFORZINI, Eszter SIMON, Etai SHPIGEL, Stephen STURZENBAUM, Etienne VERMEIRSSEN, Aldo VIARENGO, Inge WERNER a Teresa LETTIERI. Estrogenicity of chemical mixtures revealed by a panel of bioassays. \textit{Science of the Total Environment}. Amsterdam: Elsevier Science, 2021, roč.~785, September 2021, s.~1-16. ISSN~0048-9697. Dostupné z: https://dx.doi.org/10.1016/j.scitotenv.2021.147284.

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