Analysis of Treponema pallidum Strains From China Using
Improved Methods for Whole-Genome Sequencing From Primary
Syphilis Chancres

J 2021

Analysis of Treponema pallidum Strains From China Using Improved Methods for Whole-Genome Sequencing From Primary Syphilis Chancres

CHEN, W. T., David ŠMAJS, Y. F. HU, W. J. KE, Petra POSPÍŠILOVÁ et. al.

Základní údaje

Originální název

Analysis of Treponema pallidum Strains From China Using Improved Methods for Whole-Genome Sequencing From Primary Syphilis Chancres

Autoři

CHEN, W. T., David ŠMAJS (203 Česká republika, domácí), Y. F. HU, W. J. KE, Petra POSPÍŠILOVÁ (203 Česká republika, domácí), K. L. HAWLEY, M. J. CAIMANO, J. D. RADOLF, A. SENA, J. D. TUCKER, B. YANG, J. J. JULIANO, H. P. ZHENG a J. B. PARR (garant)

Vydání

The Journal of Infectious Diseases, Chicago, University of Chicago Press, 2021, 0022-1899

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 7.759

Kód RIV

RIV/00216224:14110/21:00119144

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1093/infdis/jiaa449

UT WoS

000648907800016

Klíčová slova anglicky

chancre; China; syphilis; Treponema pallidum; whole-genome sequencing

Štítky

14110513, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 8. 1. 2024 07:36, Mgr. Tereza Miškechová

Anotace

V originále

Background. Whole-genome sequencing (WGS) of lreponema pallidum subspecies pallidum (TPA) has been constrained by the lack of in vitro cultivation methods for isolating spirochetes from patient samples. Methods. We built upon recently developed enrichment methods to sequence TPA directly from primary syphilis chancre swabs collected in Guangzhou, China. Results. By combining parallel, pooled whole-genome amplification with hybrid selection, we generated high-quality genomes from 4 of 8 chancre-swab samples and 2 of 2 rabbit-passaged isolates, all subjected to challenging storage conditions. Conclusions. This approach enabled the first WGS of Chinese samples without rabbit passage and provided insights into TPA genetic diversity in China.

Návaznosti

GA17-25455S, projekt VaV

Název: Studium genomů patogenních treponem na základě analýzy jednotlivých buněk

Investor: Grantová agentura ČR, Studium genomů patogenních treponem na základě analýzy jednotlivých buněk

U19 AI144177, interní kód MU

Název: A global syphilis vaccine targeting outer membrane proteins of Treponema pallidum

Investor: Ostatní - zahraniční, A global syphilis vaccine targeting outer membrane proteins of Treponema pallidum

Citovat

CHEN, W. T., David ŠMAJS, Y. F. HU, W. J. KE, Petra POSPÍŠILOVÁ, K. L. HAWLEY, M. J. CAIMANO, J. D. RADOLF, A. SENA, J. D. TUCKER, B. YANG, J. J. JULIANO, H. P. ZHENG a J. B. PARR. Analysis of Treponema pallidum Strains From China Using Improved Methods for Whole-Genome Sequencing From Primary Syphilis Chancres. The Journal of Infectious Diseases. Chicago: University of Chicago Press, 2021, roč. 223, č. 5, s. 848-853. ISSN 0022-1899. Dostupné z: https://dx.doi.org/10.1093/infdis/jiaa449.

@article{1786839,
   author = {Chen, W. T. and Šmajs, David and Hu, Y. F. and Ke, W. J. and Pospíšilová, Petra and Hawley, K. L. and Caimano, M. J. and Radolf, J. D. and Sena, A. and Tucker, J. D. and Yang, B. and Juliano, J. J. and Zheng, H. P. and Parr, J. B.},
   article_location = {Chicago},
   article_number = {5},
   doi = {http://dx.doi.org/10.1093/infdis/jiaa449},
   keywords = {chancre; China; syphilis; Treponema pallidum; whole-genome sequencing},
   language = {eng},
   issn = {0022-1899},
   journal = {The Journal of Infectious Diseases},
   title = {Analysis of Treponema pallidum Strains From China Using Improved Methods for Whole-Genome Sequencing From Primary Syphilis Chancres},
   url = {https://academic.oup.com/jid/article-abstract/223/5/848/5876405?redirectedFrom=fulltext},
   volume = {223},
   year = {2021}
}

TY  - JOUR
ID  - 1786839
AU  - Chen, W. T. - Šmajs, David - Hu, Y. F. - Ke, W. J. - Pospíšilová, Petra - Hawley, K. L. - Caimano, M. J. - Radolf, J. D. - Sena, A. - Tucker, J. D. - Yang, B. - Juliano, J. J. - Zheng, H. P. - Parr, J. B.
PY  - 2021
TI  - Analysis of Treponema pallidum Strains From China Using Improved Methods for Whole-Genome Sequencing From Primary Syphilis Chancres
JF  - The Journal of Infectious Diseases
VL  - 223
IS  - 5
SP  - 848-853
EP  - 848-853
PB  - University of Chicago Press
SN  - 00221899
KW  - chancre
KW  - China
KW  - syphilis
KW  - Treponema pallidum
KW  - whole-genome sequencing
UR  - https://academic.oup.com/jid/article-abstract/223/5/848/5876405?redirectedFrom=fulltext
N2  - Background. Whole-genome sequencing (WGS) of lreponema pallidum subspecies pallidum (TPA) has been constrained by the lack of in vitro cultivation methods for isolating spirochetes from patient samples. Methods. We built upon recently developed enrichment methods to sequence TPA directly from primary syphilis chancre swabs collected in Guangzhou, China. Results. By combining parallel, pooled whole-genome amplification with hybrid selection, we generated high-quality genomes from 4 of 8 chancre-swab samples and 2 of 2 rabbit-passaged isolates, all subjected to challenging storage conditions. Conclusions. This approach enabled the first WGS of Chinese samples without rabbit passage and provided insights into TPA genetic diversity in China.
ER  -

CHEN, W. T., David ŠMAJS, Y. F. HU, W. J. KE, Petra POSPÍŠILOVÁ, K. L. HAWLEY, M. J. CAIMANO, J. D. RADOLF, A. SENA, J. D. TUCKER, B. YANG, J. J. JULIANO, H. P. ZHENG a J. B. PARR. Analysis of Treponema pallidum Strains From China Using Improved Methods for Whole-Genome Sequencing From Primary Syphilis Chancres. \textit{The Journal of Infectious Diseases}. Chicago: University of Chicago Press, 2021, roč.~223, č.~5, s.~848-853. ISSN~0022-1899. Dostupné z: https://dx.doi.org/10.1093/infdis/jiaa449.

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