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@article{1786886,
author = {Cermak, Vladimir and Skarkova, Aneta and Merta, Ladislav and Kolomaznikova, Veronika and Palušová, Veronika and Uldrijan, Stjepan and Rosel, Daniel and Brabek, Jan},
article_location = {BASEL},
article_number = {3},
doi = {http://dx.doi.org/10.3390/biom11030449},
keywords = {cancer; melanoma; metastasis; phenotype switch; amoeboid invasion},
language = {eng},
issn = {2218-273X},
journal = {Biomolecules},
title = {RNA-seq Characterization of Melanoma Phenotype Switch in 3D Collagen after p38 MAPK Inhibitor Treatment},
url = {https://www.mdpi.com/2218-273X/11/3/449},
volume = {11},
year = {2021}
}
TY - JOUR
ID - 1786886
AU - Cermak, Vladimir - Skarkova, Aneta - Merta, Ladislav - Kolomaznikova, Veronika - Palušová, Veronika - Uldrijan, Stjepan - Rosel, Daniel - Brabek, Jan
PY - 2021
TI - RNA-seq Characterization of Melanoma Phenotype Switch in 3D Collagen after p38 MAPK Inhibitor Treatment
JF - Biomolecules
VL - 11
IS - 3
SP - 1-13
EP - 1-13
PB - MDPI
SN - 2218273X
KW - cancer
KW - melanoma
KW - metastasis
KW - phenotype switch
KW - amoeboid invasion
UR - https://www.mdpi.com/2218-273X/11/3/449
N2 - Melanoma phenotype plasticity underlies tumour dissemination and resistance to therapy, yet its regulation is incompletely understood. In vivo switching between a more differentiated, proliferative phenotype and a dedifferentiated, invasive phenotype is directed by the tumour microenvironment. We found that treatment of partially dedifferentiated, invasive A375M2 cells with two structurally unrelated p38 MAPK inhibitors, SB2021920 and BIRB796, induces a phenotype switch in 3D collagen, as documented by increased expression of melanocyte differentiation markers and a loss of invasive phenotype markers. The phenotype is accompanied by morphological change corresponding to amoeboid-mesenchymal transition. We performed RNA sequencing with an Illumina HiSeq platform to fully characterise transcriptome changes underlying the switch. Gene expression results obtained with RNA-seq were validated by comparing them with RT-qPCR. Transcriptomic data generated in the study will extend the present understanding of phenotype plasticity in melanoma and its contribution to invasion and metastasis.
ER -
CERMAK, Vladimir, Aneta SKARKOVA, Ladislav MERTA, Veronika KOLOMAZNIKOVA, Veronika PALUŠOVÁ, Stjepan ULDRIJAN, Daniel ROSEL a Jan BRABEK. RNA-seq Characterization of Melanoma Phenotype Switch in 3D Collagen after p38 MAPK Inhibitor Treatment. \textit{Biomolecules}. BASEL: MDPI, 2021, roč.~11, č.~3, s.~1-13. ISSN~2218-273X. Dostupné z: https://dx.doi.org/10.3390/biom11030449.
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