RNA-seq Characterization of Melanoma Phenotype Switch in 3D
Collagen after p38 MAPK Inhibitor Treatment

J 2021

RNA-seq Characterization of Melanoma Phenotype Switch in 3D Collagen after p38 MAPK Inhibitor Treatment

CERMAK, Vladimir, Aneta SKARKOVA, Ladislav MERTA, Veronika KOLOMAZNIKOVA, Veronika PALUŠOVÁ et. al.

Basic information

Original name

RNA-seq Characterization of Melanoma Phenotype Switch in 3D Collagen after p38 MAPK Inhibitor Treatment

Authors

CERMAK, Vladimir (203 Czech Republic), Aneta SKARKOVA (203 Czech Republic), Ladislav MERTA (203 Czech Republic), Veronika KOLOMAZNIKOVA (203 Czech Republic), Veronika PALUŠOVÁ (703 Slovakia, belonging to the institution), Stjepan ULDRIJAN (203 Czech Republic, belonging to the institution), Daniel ROSEL (203 Czech Republic) and Jan BRABEK (203 Czech Republic)

Edition

Biomolecules, BASEL, MDPI, 2021, 2218-273X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 6.064

RIV identification code

RIV/00216224:14110/21:00122131

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.3390/biom11030449

UT WoS

000633403200001

Keywords in English

cancer; melanoma; metastasis; phenotype switch; amoeboid invasion

Abstract

V originále

Melanoma phenotype plasticity underlies tumour dissemination and resistance to therapy, yet its regulation is incompletely understood. In vivo switching between a more differentiated, proliferative phenotype and a dedifferentiated, invasive phenotype is directed by the tumour microenvironment. We found that treatment of partially dedifferentiated, invasive A375M2 cells with two structurally unrelated p38 MAPK inhibitors, SB2021920 and BIRB796, induces a phenotype switch in 3D collagen, as documented by increased expression of melanocyte differentiation markers and a loss of invasive phenotype markers. The phenotype is accompanied by morphological change corresponding to amoeboid-mesenchymal transition. We performed RNA sequencing with an Illumina HiSeq platform to fully characterise transcriptome changes underlying the switch. Gene expression results obtained with RNA-seq were validated by comparing them with RT-qPCR. Transcriptomic data generated in the study will extend the present understanding of phenotype plasticity in melanoma and its contribution to invasion and metastasis.

Citovat

CERMAK, Vladimir, Aneta SKARKOVA, Ladislav MERTA, Veronika KOLOMAZNIKOVA, Veronika PALUŠOVÁ, Stjepan ULDRIJAN, Daniel ROSEL and Jan BRABEK. RNA-seq Characterization of Melanoma Phenotype Switch in 3D Collagen after p38 MAPK Inhibitor Treatment. Biomolecules. BASEL: MDPI, 2021, vol. 11, No 3, p. 1-13. ISSN 2218-273X. Available from: https://dx.doi.org/10.3390/biom11030449.

@article{1786886,
   author = {Cermak, Vladimir and Skarkova, Aneta and Merta, Ladislav and Kolomaznikova, Veronika and Palušová, Veronika and Uldrijan, Stjepan and Rosel, Daniel and Brabek, Jan},
   article_location = {BASEL},
   article_number = {3},
   doi = {http://dx.doi.org/10.3390/biom11030449},
   keywords = {cancer; melanoma; metastasis; phenotype switch; amoeboid invasion},
   language = {eng},
   issn = {2218-273X},
   journal = {Biomolecules},
   title = {RNA-seq Characterization of Melanoma Phenotype Switch in 3D Collagen after p38 MAPK Inhibitor Treatment},
   url = {https://www.mdpi.com/2218-273X/11/3/449},
   volume = {11},
   year = {2021}
}

TY  - JOUR
ID  - 1786886
AU  - Cermak, Vladimir - Skarkova, Aneta - Merta, Ladislav - Kolomaznikova, Veronika - Palušová, Veronika - Uldrijan, Stjepan - Rosel, Daniel - Brabek, Jan
PY  - 2021
TI  - RNA-seq Characterization of Melanoma Phenotype Switch in 3D Collagen after p38 MAPK Inhibitor Treatment
JF  - Biomolecules
VL  - 11
IS  - 3
SP  - 1-13
EP  - 1-13
PB  - MDPI
SN  - 2218273X
KW  - cancer
KW  - melanoma
KW  - metastasis
KW  - phenotype switch
KW  - amoeboid invasion
UR  - https://www.mdpi.com/2218-273X/11/3/449
N2  - Melanoma phenotype plasticity underlies tumour dissemination and resistance to therapy, yet its regulation is incompletely understood. In vivo switching between a more differentiated, proliferative phenotype and a dedifferentiated, invasive phenotype is directed by the tumour microenvironment. We found that treatment of partially dedifferentiated, invasive A375M2 cells with two structurally unrelated p38 MAPK inhibitors, SB2021920 and BIRB796, induces a phenotype switch in 3D collagen, as documented by increased expression of melanocyte differentiation markers and a loss of invasive phenotype markers. The phenotype is accompanied by morphological change corresponding to amoeboid-mesenchymal transition. We performed RNA sequencing with an Illumina HiSeq platform to fully characterise transcriptome changes underlying the switch. Gene expression results obtained with RNA-seq were validated by comparing them with RT-qPCR. Transcriptomic data generated in the study will extend the present understanding of phenotype plasticity in melanoma and its contribution to invasion and metastasis.
ER  -

CERMAK, Vladimir, Aneta SKARKOVA, Ladislav MERTA, Veronika KOLOMAZNIKOVA, Veronika PALUŠOVÁ, Stjepan ULDRIJAN, Daniel ROSEL and Jan BRABEK. RNA-seq Characterization of Melanoma Phenotype Switch in 3D Collagen after p38 MAPK Inhibitor Treatment. \textit{Biomolecules}. BASEL: MDPI, 2021, vol.~11, No~3, p.~1-13. ISSN~2218-273X. Available from: https://dx.doi.org/10.3390/biom11030449.

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