T-Cell Activation: Post-Infection Diagnostic Tool for COVID-19

J 2021

T-Cell Activation: Post-Infection Diagnostic Tool for COVID-19

ŠIMARA, Pavel, Lenka TESAŘOVÁ, Ivana ŤAPUCHOVÁ, J. CELEROVA, Irena KOUTNÁ et. al.

Základní údaje

Originální název

T-Cell Activation: Post-Infection Diagnostic Tool for COVID-19

Autoři

ŠIMARA, Pavel (203 Česká republika, domácí), Lenka TESAŘOVÁ (203 Česká republika, domácí), Ivana ŤAPUCHOVÁ (703 Slovensko, domácí), J. CELEROVA (203 Česká republika) a Irena KOUTNÁ (203 Česká republika, garant, domácí)

Vydání

Folia biologica, Praha, Institute of Molecular Genetics, 2021, 0015-5500

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.167

Kód RIV

RIV/00216224:14110/21:00122151

Organizační jednotka

Lékařská fakulta

UT WoS

000672501500002

Klíčová slova anglicky

COVID-19; SARS-CoV-2; T-cell activation

Štítky

14110517, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 8. 2021 09:49, Mgr. Tereza Miškechová

Anotace

V originále

COVID-19 is caused by the SARS-CoV-2 virus and has spread globally in 2020. Cellular immunity may serve as an important functional marker of the disease, especially in the asymptomatic cases. Blood samples were collected from 46 convalescent donors with a history of COVID-19 and 38 control donors. Quantification of the T-cell response upon contact with SARS-CoV-2 proteins in vitro was based on IFN-y. Significantly higher numbers of activated cells were measured in patients who underwent COVID-19. Anti-SARS-CoV-2 T cells were detected weeks after the active virus disappeared from the organism. Repeated sample collection after five months proved that the T-cell activation was weaker in time in 79 % of the patients. In the majority of cases, the CD4(+) helper T-cell subpopulation was responsible for the immune reaction. Moreover, different viral proteins triggered activation in CD4(+) helper and in CD8(+) cytotoxic T cells. Together, these findings suggest that the T-cell activation level identifies the individuals who underwent COVID-19 and may become a diagnostic tool for the disease.

Návaznosti

LM2018133, projekt VaV

Název: Český národní uzel Evropské infrastruktury pro translační medicínu (Akronym: EATRIS-ERIC-CZ)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Involvement of Czech Translational Medicine Infrastructure to the European Advanced Translational Research Infrastructure in Medicine

Citovat

ŠIMARA, Pavel, Lenka TESAŘOVÁ, Ivana ŤAPUCHOVÁ, J. CELEROVA a Irena KOUTNÁ. T-Cell Activation: Post-Infection Diagnostic Tool for COVID-19. Folia biologica. Praha: Institute of Molecular Genetics, 2021, roč. 67, č. 1, s. 16-27. ISSN 0015-5500.

@article{1787481,
   author = {Šimara, Pavel and Tesařová, Lenka and Ťapuchová, Ivana and Celerova, J. and Koutná, Irena},
   article_location = {Praha},
   article_number = {1},
   keywords = {COVID-19; SARS-CoV-2; T-cell activation},
   language = {eng},
   issn = {0015-5500},
   journal = {Folia biologica},
   title = {T-Cell Activation: Post-Infection Diagnostic Tool for COVID-19},
   url = {https://fb.cuni.cz/file/5943/fb2021a0003.pdf},
   volume = {67},
   year = {2021}
}

TY  - JOUR
ID  - 1787481
AU  - Šimara, Pavel - Tesařová, Lenka - Ťapuchová, Ivana - Celerova, J. - Koutná, Irena
PY  - 2021
TI  - T-Cell Activation: Post-Infection Diagnostic Tool for COVID-19
JF  - Folia biologica
VL  - 67
IS  - 1
SP  - 16-27
EP  - 16-27
PB  - Institute of Molecular Genetics
SN  - 00155500
KW  - COVID-19
KW  - SARS-CoV-2
KW  - T-cell activation
UR  - https://fb.cuni.cz/file/5943/fb2021a0003.pdf
N2  - COVID-19 is caused by the SARS-CoV-2 virus and has spread globally in 2020. Cellular immunity may serve as an important functional marker of the disease, especially in the asymptomatic cases. Blood samples were collected from 46 convalescent donors with a history of COVID-19 and 38 control donors. Quantification of the T-cell response upon contact with SARS-CoV-2 proteins in vitro was based on IFN-y. Significantly higher numbers of activated cells were measured in patients who underwent COVID-19. Anti-SARS-CoV-2 T cells were detected weeks after the active virus disappeared from the organism. Repeated sample collection after five months proved that the T-cell activation was weaker in time in 79 % of the patients. In the majority of cases, the CD4(+) helper T-cell subpopulation was responsible for the immune reaction. Moreover, different viral proteins triggered activation in CD4(+) helper and in CD8(+) cytotoxic T cells. Together, these findings suggest that the T-cell activation level identifies the individuals who underwent COVID-19 and may become a diagnostic tool for the disease.
ER  -

ŠIMARA, Pavel, Lenka TESAŘOVÁ, Ivana ŤAPUCHOVÁ, J. CELEROVA a Irena KOUTNÁ. T-Cell Activation: Post-Infection Diagnostic Tool for COVID-19. \textit{Folia biologica}. Praha: Institute of Molecular Genetics, 2021, roč.~67, č.~1, s.~16-27. ISSN~0015-5500.

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