2021
Oxygen Is an Ambivalent Factor for the Differentiation of Human Pluripotent Stem Cells in Cardiac 2D Monolayer and 3D Cardiac Spheroids
SOUIDI, M., Y. SLEIMAN, Ivana AĆIMOVIĆ, Jan PŘIBYL, A. CHARRABI et. al.Základní údaje
Originální název
Oxygen Is an Ambivalent Factor for the Differentiation of Human Pluripotent Stem Cells in Cardiac 2D Monolayer and 3D Cardiac Spheroids
Autoři
SOUIDI, M., Y. SLEIMAN, Ivana AĆIMOVIĆ (688 Srbsko, domácí), Jan PŘIBYL (203 Česká republika, domácí), A. CHARRABI, V. BAECKER, V. SCHEUERMANN, Martin PEŠL (203 Česká republika, garant, domácí), Šárka JELÍNKOVÁ (203 Česká republika, domácí), Petr SKLÁDAL (203 Česká republika, domácí), Petr DVOŘÁK (203 Česká republika, domácí), A. LACAMPAGNE, Vladimír ROTREKL (203 Česká republika, domácí) a A. C. MELI
Vydání
International Journal of Molecular Sciences, Basel, Multidisciplinary Digital Publishing Institute, 2021, 1422-0067
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
10608 Biochemistry and molecular biology
Stát vydavatele
Švýcarsko
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Impakt faktor
Impact factor: 6.208
Kód RIV
RIV/00216224:14740/21:00120132
Organizační jednotka
Středoevropský technologický institut
UT WoS
000611336200001
Klíčová slova anglicky
hPSC-derived cardiomyocytes; cardiac spheroids; embryoid bodies; 2D-monolayer; oxygen exposure; intracellular calcium handling; mitochondrial oxygen consumption; contractile properties
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 15. 10. 2024 09:27, Ing. Monika Szurmanová, Ph.D.
Anotace
V originále
Numerous protocols of cardiac differentiation have been established by essentially focusing on specific growth factors on human pluripotent stem cell (hPSC) differentiation efficiency. However, the optimal environmental factors to obtain cardiac myocytes in network are still unclear. The mesoderm germ layer differentiation is known to be enhanced by low oxygen exposure. Here, we hypothesized that low oxygen exposure enhances the molecular and functional maturity of the cardiomyocytes. We aimed at comparing the molecular and functional consequences of low (5% O-2 or LOE) and high oxygen exposure (21% O-2 or HOE) on cardiac differentiation of hPSCs in 2D- and 3D-based protocols. hPSC-CMs were differentiated through both the 2D (monolayer) and 3D (embryoid body) protocols using several lines. Cardiac marker expression and cell morphology were assessed. The mitochondrial localization and metabolic properties were evaluated. The intracellular Ca2+ handling and contractile properties were also monitored. The 2D cardiac monolayer can only be differentiated in HOE. The 3D cardiac spheroids containing hPSC-CMs in LOE further exhibited cardiac markers, hypertrophy, steadier SR Ca2+ release properties revealing a better SR Ca2+ handling, and enhanced contractile force. Preserved distribution of mitochondria and similar oxygen consumption by the mitochondrial respiratory chain complexes were also observed. Our results brought evidences that LOE is moderately beneficial for the 3D cardiac spheroids with hPSC-CMs exhibiting further maturity. In contrast, the 2D cardiac monolayers strictly require HOE.
Návaznosti
LM2018127, projekt VaV |
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NU20-06-00156, projekt VaV |
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2SGA2744, interní kód MU |
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90127, velká výzkumná infrastruktura |
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