Microcomputed tomographic, biomechanical and histological
analyses of lumbar interbody fusion with iliac crest bone graft
in a pig model

J 2021

Microcomputed tomographic, biomechanical and histological analyses of lumbar interbody fusion with iliac crest bone graft in a pig model

KRTIČKA, Milan, Vladimír NEKUDA, Daniel IRA, Radek SEDLACEK, Tomas SUCHY et. al.

Základní údaje

Originální název

Microcomputed tomographic, biomechanical and histological analyses of lumbar interbody fusion with iliac crest bone graft in a pig model

Autoři

KRTIČKA, Milan (203 Česká republika, domácí), Vladimír NEKUDA (203 Česká republika, domácí), Daniel IRA (703 Slovensko, domácí), Radek SEDLACEK (203 Česká republika), Tomas SUCHY (203 Česká republika), Michaela KAVKOVA (203 Česká republika), Adam BRINEK (203 Česká republika), Eduard GOPFRT (203 Česká republika), Andrej BILIK, Jozef KAISER, Leoš KŘEN (203 Česká republika, domácí) a Ladislav PLÁNKA (203 Česká republika, garant, domácí)

Vydání

Acta veterinaria (Brno), Brno, VETERINARNI A FARMACEUTICKA UNIVERZITA BRNO, 2021, 0001-7213

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

40301 Veterinary science

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 0.817

Kód RIV

RIV/00216224:14110/21:00122157

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.2754/avb202190010069

UT WoS

000654628800009

Klíčová slova anglicky

Bone replacement; spine; injury

Štítky

14110230, 14110232, 14110313, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 8. 2021 13:13, Mgr. Tereza Miškechová

Anotace

V originále

The main goal of this study was to assess the progress of vertebral stability after lumbar interbody fusion related to microcomputed tomography (micro CT), biomechanical analysis, and histological assessment towards spine fusion. Twelve male pigs were used; each underwent L2-3 discectomy and implantation of an iliac crest bone graft in two groups; six spines were harvested eight weeks (A1) and six spines 16 weeks (A2) after surgery (7 native spines for biomechanical analysis). The CT was performed by GE phoenix datos vertical bar x 2.0 with a sample drift correction. The samples were divided according to fusion quality. Biomechanical evaluation was carried out on the MTS Mini Bionix testing system. In the nondestructive mode, three cycles of pure bending moments were applied (5 Nm load limit) at a rate of 20 degrees/min in flexion (+40 degrees) and extension (-40 degrees). Two representative histological sections from four samples were obtained (A1, n = 2; A2, n = 2); areas of mature bone were quantified. In micro CT, better results were achieved in group A2 (not significant). Eight weeks after the operation, flexural stiffness decreased to 48% of its initial value for native cadavers (P < 0.05); after 16 weeks it was comparable to native cadavers, demonstrating the suitability of the implanted graft (P < 0.05). The newly formed bone tissue occupied an average area of 94.205 mm(2) (A1) and 26.240 mm(2) (A2). It was confirmed that micro CT, biomechanical analysis, and histological assessment are technically feasible and suitable for the evaluation of results of other methods of large bone defect treatment.

Citovat

KRTIČKA, Milan, Vladimír NEKUDA, Daniel IRA, Radek SEDLACEK, Tomas SUCHY, Michaela KAVKOVA, Adam BRINEK, Eduard GOPFRT, Andrej BILIK, Jozef KAISER, Leoš KŘEN a Ladislav PLÁNKA. Microcomputed tomographic, biomechanical and histological analyses of lumbar interbody fusion with iliac crest bone graft in a pig model. Acta veterinaria (Brno). Brno: VETERINARNI A FARMACEUTICKA UNIVERZITA BRNO, 2021, roč. 90, č. 1, s. 69-75. ISSN 0001-7213. Dostupné z: https://dx.doi.org/10.2754/avb202190010069.

@article{1787519,
   author = {Krtička, Milan and Nekuda, Vladimír and Ira, Daniel and Sedlacek, Radek and Suchy, Tomas and Kavkova, Michaela and Brinek, Adam and Gopfrt, Eduard and Bilik, Andrej and Kaiser, Jozef and Křen, Leoš and Plánka, Ladislav},
   article_location = {Brno},
   article_number = {1},
   doi = {http://dx.doi.org/10.2754/avb202190010069},
   keywords = {Bone replacement; spine; injury},
   language = {eng},
   issn = {0001-7213},
   journal = {Acta veterinaria (Brno)},
   title = {Microcomputed tomographic, biomechanical and histological analyses of lumbar interbody fusion with iliac crest bone graft in a pig model},
   url = {https://actavet.vfu.cz/90/1/0069/},
   volume = {90},
   year = {2021}
}

TY  - JOUR
ID  - 1787519
AU  - Krtička, Milan - Nekuda, Vladimír - Ira, Daniel - Sedlacek, Radek - Suchy, Tomas - Kavkova, Michaela - Brinek, Adam - Gopfrt, Eduard - Bilik, Andrej - Kaiser, Jozef - Křen, Leoš - Plánka, Ladislav
PY  - 2021
TI  - Microcomputed tomographic, biomechanical and histological analyses of lumbar interbody fusion with iliac crest bone graft in a pig model
JF  - Acta veterinaria (Brno)
VL  - 90
IS  - 1
SP  - 69-75
EP  - 69-75
PB  - VETERINARNI A FARMACEUTICKA UNIVERZITA BRNO
SN  - 00017213
KW  - Bone replacement
KW  - spine
KW  - injury
UR  - https://actavet.vfu.cz/90/1/0069/
N2  - The main goal of this study was to assess the progress of vertebral stability after lumbar interbody fusion related to microcomputed tomography (micro CT), biomechanical analysis, and histological assessment towards spine fusion. Twelve male pigs were used; each underwent L2-3 discectomy and implantation of an iliac crest bone graft in two groups; six spines were harvested eight weeks (A1) and six spines 16 weeks (A2) after surgery (7 native spines for biomechanical analysis). The CT was performed by GE phoenix datos vertical bar x 2.0 with a sample drift correction. The samples were divided according to fusion quality. Biomechanical evaluation was carried out on the MTS Mini Bionix testing system. In the nondestructive mode, three cycles of pure bending moments were applied (5 Nm load limit) at a rate of 20 degrees/min in flexion (+40 degrees) and extension (-40 degrees). Two representative histological sections from four samples were obtained (A1, n = 2; A2, n = 2); areas of mature bone were quantified. In micro CT, better results were achieved in group A2 (not significant). Eight weeks after the operation, flexural stiffness decreased to 48% of its initial value for native cadavers (P < 0.05); after 16 weeks it was comparable to native cadavers, demonstrating the suitability of the implanted graft (P < 0.05). The newly formed bone tissue occupied an average area of 94.205 mm(2) (A1) and 26.240 mm(2) (A2). It was confirmed that micro CT, biomechanical analysis, and histological assessment are technically feasible and suitable for the evaluation of results of other methods of large bone defect treatment.
ER  -

KRTIČKA, Milan, Vladimír NEKUDA, Daniel IRA, Radek SEDLACEK, Tomas SUCHY, Michaela KAVKOVA, Adam BRINEK, Eduard GOPFRT, Andrej BILIK, Jozef KAISER, Leoš KŘEN a Ladislav PLÁNKA. Microcomputed tomographic, biomechanical and histological analyses of lumbar interbody fusion with iliac crest bone graft in a pig model. \textit{Acta veterinaria (Brno)}. Brno: VETERINARNI A FARMACEUTICKA UNIVERZITA BRNO, 2021, roč.~90, č.~1, s.~69-75. ISSN~0001-7213. Dostupné z: https://dx.doi.org/10.2754/avb202190010069.

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