Is centrifugal ultrafiltration a robust method for determining
encapsulation efficiency of pesticide nanoformulations?

J 2021

Is centrifugal ultrafiltration a robust method for determining encapsulation efficiency of pesticide nanoformulations?

LÓPEZ CABEZA, María del Rocío, Mélanie Marie KAH, Renato GRILLO, Zuzana BÍLKOVÁ, Jakub HOFMAN et. al.

Basic information

Original name

Is centrifugal ultrafiltration a robust method for determining encapsulation efficiency of pesticide nanoformulations?

Authors

LÓPEZ CABEZA, María del Rocío (724 Spain, belonging to the institution), Mélanie Marie KAH (250 France), Renato GRILLO (76 Brazil), Zuzana BÍLKOVÁ (203 Czech Republic, belonging to the institution) and Jakub HOFMAN (203 Czech Republic, guarantor, belonging to the institution)

Edition

Nanoscale, Cambridge, Royal Society of Chemistry, 2021, 2040-3364

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10400 1.4 Chemical sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 8.307

RIV identification code

RIV/00216224:14310/21:00119168

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1039/d0nr08693b

UT WoS

000630384400023

Keywords in English

Pesticides; nanoparticles; ultrafiltration

Abstract

V originále

Loading active ingredients on nanocarrier systems is becoming a common strategy for improving pesticide formulations. One of the most important properties of these nanoformulations is the proportion of pesticide associated with the nanocarriers (encapsulation efficiency, EE). EE is often determined by centrifugal ultrafiltration. However, the losses of active ingredient in the centrifugal ultrafiltration devices are typically not assessed, potentially leading to erroneous results. In this work, the losses of three pesticides (tebuconazole, terbuthylazine and chlorpyrifos) during centrifugal ultrafiltration have been systematically evaluated for nine different devices. Results suggest that centrifugal ultrafiltration is not suitable for determining the EE of compounds such as chlorpyrifos as 100% losses were observed on all the devices tested. Losses of tebuconazole and terbuthylazine were highly variable according to the type of membrane and the lowest losses were observed in the devices with hydrophilic regenerated cellulose membranes. Based on these results, we propose a correction factor and demonstrate its application to calculate the EE of two nanoformulations based on poly(epsilon-caprolactone) nanocarriers. The approach extends the applicability of centrifugal ultrafiltration to a wider range of pesticide nanoformulations. We also discuss the effect of dilution on EE and make recommendations to improve the characterisation of nanoparticles-based pesticide nanoformulations in the future.

Links

EF17_043/0009632, research and development project

Name: CETOCOEN Excellence

GA18-19324S, research and development project

Name: Nanopesticidy v životním prostředí – porozumění jejich osudu, biodostupnosti, účinkům a rizikům v půdě a vodě

Investor: Czech Science Foundation

LM2018121, research and development project

Name: Výzkumná infrastruktura RECETOX (Acronym: RECETOX RI)

Investor: Ministry of Education, Youth and Sports of the CR, RECETOX RI

Citovat

LÓPEZ CABEZA, María del Rocío, Mélanie Marie KAH, Renato GRILLO, Zuzana BÍLKOVÁ and Jakub HOFMAN. Is centrifugal ultrafiltration a robust method for determining encapsulation efficiency of pesticide nanoformulations? Nanoscale. Cambridge: Royal Society of Chemistry, 2021, vol. 13, No 10, p. 5410-5418. ISSN 2040-3364. Available from: https://dx.doi.org/10.1039/d0nr08693b.

@article{1789119,
   author = {López Cabeza, María del Rocío and Kah, Mélanie Marie and Grillo, Renato and Bílková, Zuzana and Hofman, Jakub},
   article_location = {Cambridge},
   article_number = {10},
   doi = {http://dx.doi.org/10.1039/d0nr08693b},
   keywords = {Pesticides; nanoparticles; ultrafiltration},
   language = {eng},
   issn = {2040-3364},
   journal = {Nanoscale},
   title = {Is centrifugal ultrafiltration a robust method for determining encapsulation efficiency of pesticide nanoformulations?},
   url = {https://pubs.rsc.org/en/content/articlelanding/2021/NR/D0NR08693B},
   volume = {13},
   year = {2021}
}

TY  - JOUR
ID  - 1789119
AU  - López Cabeza, María del Rocío - Kah, Mélanie Marie - Grillo, Renato - Bílková, Zuzana - Hofman, Jakub
PY  - 2021
TI  - Is centrifugal ultrafiltration a robust method for determining encapsulation efficiency of pesticide nanoformulations?
JF  - Nanoscale
VL  - 13
IS  - 10
SP  - 5410-5418
EP  - 5410-5418
PB  - Royal Society of Chemistry
SN  - 20403364
KW  - Pesticides
KW  - nanoparticles
KW  - ultrafiltration
UR  - https://pubs.rsc.org/en/content/articlelanding/2021/NR/D0NR08693B
N2  - Loading active ingredients on nanocarrier systems is becoming a common strategy for improving pesticide formulations. One of the most important properties of these nanoformulations is the proportion of pesticide associated with the nanocarriers (encapsulation efficiency, EE). EE is often determined by centrifugal ultrafiltration. However, the losses of active ingredient in the centrifugal ultrafiltration devices are typically not assessed, potentially leading to erroneous results. In this work, the losses of three pesticides (tebuconazole, terbuthylazine and chlorpyrifos) during centrifugal ultrafiltration have been systematically evaluated for nine different devices. Results suggest that centrifugal ultrafiltration is not suitable for determining the EE of compounds such as chlorpyrifos as 100% losses were observed on all the devices tested. Losses of tebuconazole and terbuthylazine were highly variable according to the type of membrane and the lowest losses were observed in the devices with hydrophilic regenerated cellulose membranes. Based on these results, we propose a correction factor and demonstrate its application to calculate the EE of two nanoformulations based on poly(epsilon-caprolactone) nanocarriers. The approach extends the applicability of centrifugal ultrafiltration to a wider range of pesticide nanoformulations. We also discuss the effect of dilution on EE and make recommendations to improve the characterisation of nanoparticles-based pesticide nanoformulations in the future.
ER  -

LÓPEZ CABEZA, María del Rocío, Mélanie Marie KAH, Renato GRILLO, Zuzana BÍLKOVÁ and Jakub HOFMAN. Is centrifugal ultrafiltration a robust method for determining encapsulation efficiency of pesticide nanoformulations? \textit{Nanoscale}. Cambridge: Royal Society of Chemistry, 2021, vol.~13, No~10, p.~5410-5418. ISSN~2040-3364. Available from: https://dx.doi.org/10.1039/d0nr08693b.

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