Variation in Ribosomal DNA in the Genus Trifolium (Fabaceae)

J 2021

Variation in Ribosomal DNA in the Genus Trifolium (Fabaceae)

VOZÁROVÁ, Radka, Eliška MACKOVÁ, David VLK a Jana ŘEPKOVÁ

Základní údaje

Originální název

Variation in Ribosomal DNA in the Genus Trifolium (Fabaceae)

Autoři

VOZÁROVÁ, Radka (703 Slovensko, domácí), Eliška MACKOVÁ (203 Česká republika, domácí), David VLK (203 Česká republika, domácí) a Jana ŘEPKOVÁ (203 Česká republika, garant, domácí)

Vydání

Plants, MDPI, 2021, 2223-7747

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.658

Kód RIV

RIV/00216224:14310/21:00122222

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.3390/plants10091771

UT WoS

000701576700001

Klíčová slova anglicky

5S rDNA; 26S rDNA; clover; fluorescent in situ hybridization; nucleotide polymorphism; genome structure

Štítky

CF SAXS

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 17. 2. 2023 09:11, prof. RNDr. Jana Řepková, CSc.

Anotace

V originále

The genus Trifolium L. is characterized by basic chromosome numbers 8, 7, 6, and 5. We conducted a genus-wide study of ribosomal DNA (rDNA) structure variability in diploids and polyploids to gain insight into evolutionary history. We used fluorescent in situ hybridization to newly investigate rDNA variation by number and position in 30 Trifolium species. Evolutionary history among species was examined using 85 available sequences of internal transcribed spacer 1 (ITS1) of 35S rDNA. In diploid species with ancestral basic chromosome number (x = 8), one pair of 5S and 26S rDNA in separate or adjacent positions on a pair of chromosomes was prevalent. Genomes of species with reduced basic chromosome numbers were characterized by increased number of signals determined on one pair of chromosomes or all chromosomes. Increased number of signals was observed also in diploids Trifolium alpestre and Trifolium microcephalum and in polyploids. Sequence alignment revealed ITS1 sequences with mostly single nucleotide polymorphisms, and ITS1 diversity was greater in diploids with reduced basic chromosome numbers compared to diploids with ancestral basic chromosome number (x = 8) and polyploids. Our results suggest the presence of one 5S rDNA site and one 26S rDNA site as an ancestral state.

Návaznosti

MUNI/A/1522/2020, interní kód MU

Název: Podpora výzkumné činnosti studentů molekulární biologie a genetiky 9 (Akronym: MBG9)

Investor: Masarykova univerzita, Podpora výzkumné činnosti studentů molekulární biologie a genetiky 9

Citovat

VOZÁROVÁ, Radka, Eliška MACKOVÁ, David VLK a Jana ŘEPKOVÁ. Variation in Ribosomal DNA in the Genus Trifolium (Fabaceae). Plants. MDPI, 2021, roč. 10, č. 9, s. "1771", 16 s. ISSN 2223-7747. Dostupné z: https://dx.doi.org/10.3390/plants10091771.

@article{1789338,
   author = {Vozárová, Radka and Macková, Eliška and Vlk, David and Řepková, Jana},
   article_number = {9},
   doi = {http://dx.doi.org/10.3390/plants10091771},
   keywords = {5S rDNA; 26S rDNA; clover; fluorescent in situ hybridization; nucleotide polymorphism; genome structure},
   language = {eng},
   issn = {2223-7747},
   journal = {Plants},
   title = {Variation in Ribosomal DNA in the Genus Trifolium (Fabaceae)},
   url = {https://doi.org/10.3390/plants10091771},
   volume = {10},
   year = {2021}
}

TY  - JOUR
ID  - 1789338
AU  - Vozárová, Radka - Macková, Eliška - Vlk, David - Řepková, Jana
PY  - 2021
TI  - Variation in Ribosomal DNA in the Genus Trifolium (Fabaceae)
JF  - Plants
VL  - 10
IS  - 9
SP  - "1771"
EP  - "1771"
PB  - MDPI
SN  - 22237747
KW  - 5S rDNA
KW  - 26S rDNA
KW  - clover
KW  - fluorescent in situ hybridization
KW  - nucleotide polymorphism
KW  - genome structure
UR  - https://doi.org/10.3390/plants10091771
N2  - The genus Trifolium L. is characterized by basic chromosome numbers 8, 7, 6, and 5. We conducted a genus-wide study of ribosomal DNA (rDNA) structure variability in diploids and polyploids to gain insight into evolutionary history. We used fluorescent in situ hybridization to newly investigate rDNA variation by number and position in 30 Trifolium species. Evolutionary history among species was examined using 85 available sequences of internal transcribed spacer 1 (ITS1) of 35S rDNA. In diploid species with ancestral basic chromosome number (x = 8), one pair of 5S and 26S rDNA in separate or adjacent positions on a pair of chromosomes was prevalent. Genomes of species with reduced basic chromosome numbers were characterized by increased number of signals determined on one pair of chromosomes or all chromosomes. Increased number of signals was observed also in diploids Trifolium alpestre and Trifolium microcephalum and in polyploids. Sequence alignment revealed ITS1 sequences with mostly single nucleotide polymorphisms, and ITS1 diversity was greater in diploids with reduced basic chromosome numbers compared to diploids with ancestral basic chromosome number (x = 8) and polyploids. Our results suggest the presence of one 5S rDNA site and one 26S rDNA site as an ancestral state.
ER  -

VOZÁROVÁ, Radka, Eliška MACKOVÁ, David VLK a Jana ŘEPKOVÁ. Variation in Ribosomal DNA in the Genus Trifolium (Fabaceae). \textit{Plants}. MDPI, 2021, roč.~10, č.~9, s.~''1771'', 16 s. ISSN~2223-7747. Dostupné z: https://dx.doi.org/10.3390/plants10091771.

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