Plasmacytoid Dendritic Cells in Patients with MGUS and Multiple
Myeloma

J 2021

Plasmacytoid Dendritic Cells in Patients with MGUS and Multiple Myeloma

KNIGHT, Andrea, Lucie ŘÍHOVÁ, Romana KRÁLOVÁ, Miroslav PENKA, Zdeněk ADAM et. al.

Základní údaje

Originální název

Plasmacytoid Dendritic Cells in Patients with MGUS and Multiple Myeloma

Autoři

KNIGHT, Andrea (203 Česká republika, garant, domácí), Lucie ŘÍHOVÁ (203 Česká republika), Romana KRÁLOVÁ (203 Česká republika), Miroslav PENKA (203 Česká republika), Zdeněk ADAM (203 Česká republika), Luděk POUR (203 Česká republika), Martin PISKÁČEK (40 Rakousko, domácí) a Roman HAJEK (203 Česká republika)

Vydání

Journal of Clinical Medicine, Basel, MDPI, 2021, 2077-0383

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30218 General and internal medicine

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.964

Kód RIV

RIV/00216224:14110/21:00120139

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.3390/jcm10163717

UT WoS

000689192700001

Klíčová slova anglicky

plasmacytoid dendritic cells; MGUS; multiple myeloma; immunosuppressive tumor microenvironment

Štítky

14110518, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 9. 9. 2021 06:57, Mgr. Tereza Miškechová

Anotace

V originále

Background: Plasmacytoid dendritic cells (pDCs) play prominent roles in mediating innate and adaptive immune responses. However, it is unclear how pDCs contribute to the immunosuppressive tumor microenvironment described in multiple myeloma (MM). Methods: Newly diagnosed myeloma patients (MM, n = 37) were analyzed to determine the pDC counts in comparison to peripheral blood (PB, n = 53) and bone marrow (BM, n = 10) samples of age-matched healthy donors (HD) using flow cytometry. Second, proliferation of myeloma tumor cells in the presence of freshly isolated pDCs was examined. Third, production of IFN alpha by pDCs co-cultured with MM cells was determined by intracellular staining. Results: We found a highly significant reduction of circulating pDCs (p < 0.0001) and in bone marrow (p < 0.0001) of MM patients compared to HD. We also observed a significant decrease of pDCs (p = 0.004) in BM in patients with monoclonal gammopathy of undetermined significance (MGUS, n = 12). Importantly, we determined that pDCs promote proliferation specifically of MM cells and not the stromal cells and that pDCs secrete IFN alpha upon co-culture with MM tumor cells. Conclusions: Our results show altered pDC frequencies in the BM microenvironment in MGUS and MM patients at diagnosis. We showed the tumor-promoting function of pDCs that may mediate immune deficiencies affecting long-term disease control and treatment outcome.

Návaznosti

NV15-32935A, projekt VaV

Název: Analýza gamma-delta T lymfocytů reaktivních na nádorové buňky u pacientů s B-buněčnou chronickou lymfocytární leukémií: nový přístup k buněčné terapii

NV19-05-00410, projekt VaV

Název: Úloha cytotoxických gamma-delta T buněk na terapeutické rezistenci a recidivě Glioblastoma Multiforme

Investor: Ministerstvo zdravotnictví ČR, Úloha cytotoxických gamma-delta T buněk na terapeutické rezistenci a recidivě Glioblastoma Multiforme

Citovat

KNIGHT, Andrea, Lucie ŘÍHOVÁ, Romana KRÁLOVÁ, Miroslav PENKA, Zdeněk ADAM, Luděk POUR, Martin PISKÁČEK a Roman HAJEK. Plasmacytoid Dendritic Cells in Patients with MGUS and Multiple Myeloma. Journal of Clinical Medicine. Basel: MDPI, 2021, roč. 10, č. 16, s. 1-11. ISSN 2077-0383. Dostupné z: https://dx.doi.org/10.3390/jcm10163717.

@article{1790617,
   author = {Knight, Andrea and Říhová, Lucie and Králová, Romana and Penka, Miroslav and Adam, Zdeněk and Pour, Luděk and Piskáček, Martin and Hajek, Roman},
   article_location = {Basel},
   article_number = {16},
   doi = {http://dx.doi.org/10.3390/jcm10163717},
   keywords = {plasmacytoid dendritic cells; MGUS; multiple myeloma; immunosuppressive tumor microenvironment},
   language = {eng},
   issn = {2077-0383},
   journal = {Journal of Clinical Medicine},
   title = {Plasmacytoid Dendritic Cells in Patients with MGUS and Multiple Myeloma},
   url = {https://www.mdpi.com/2077-0383/10/16/3717},
   volume = {10},
   year = {2021}
}

TY  - JOUR
ID  - 1790617
AU  - Knight, Andrea - Říhová, Lucie - Králová, Romana - Penka, Miroslav - Adam, Zdeněk - Pour, Luděk - Piskáček, Martin - Hajek, Roman
PY  - 2021
TI  - Plasmacytoid Dendritic Cells in Patients with MGUS and Multiple Myeloma
JF  - Journal of Clinical Medicine
VL  - 10
IS  - 16
SP  - 1-11
EP  - 1-11
PB  - MDPI
SN  - 20770383
KW  - plasmacytoid dendritic cells
KW  - MGUS
KW  - multiple myeloma
KW  - immunosuppressive tumor microenvironment
UR  - https://www.mdpi.com/2077-0383/10/16/3717
N2  - Background: Plasmacytoid dendritic cells (pDCs) play prominent roles in mediating innate and adaptive immune responses. However, it is unclear how pDCs contribute to the immunosuppressive tumor microenvironment described in multiple myeloma (MM). Methods: Newly diagnosed myeloma patients (MM, n = 37) were analyzed to determine the pDC counts in comparison to peripheral blood (PB, n = 53) and bone marrow (BM, n = 10) samples of age-matched healthy donors (HD) using flow cytometry. Second, proliferation of myeloma tumor cells in the presence of freshly isolated pDCs was examined. Third, production of IFN alpha by pDCs co-cultured with MM cells was determined by intracellular staining. Results: We found a highly significant reduction of circulating pDCs (p < 0.0001) and in bone marrow (p < 0.0001) of MM patients compared to HD. We also observed a significant decrease of pDCs (p = 0.004) in BM in patients with monoclonal gammopathy of undetermined significance (MGUS, n = 12). Importantly, we determined that pDCs promote proliferation specifically of MM cells and not the stromal cells and that pDCs secrete IFN alpha upon co-culture with MM tumor cells. Conclusions: Our results show altered pDC frequencies in the BM microenvironment in MGUS and MM patients at diagnosis. We showed the tumor-promoting function of pDCs that may mediate immune deficiencies affecting long-term disease control and treatment outcome.
ER  -

KNIGHT, Andrea, Lucie ŘÍHOVÁ, Romana KRÁLOVÁ, Miroslav PENKA, Zdeněk ADAM, Luděk POUR, Martin PISKÁČEK a Roman HAJEK. Plasmacytoid Dendritic Cells in Patients with MGUS and Multiple Myeloma. \textit{Journal of Clinical Medicine}. Basel: MDPI, 2021, roč.~10, č.~16, s.~1-11. ISSN~2077-0383. Dostupné z: https://dx.doi.org/10.3390/jcm10163717.

Podrobný výpis o publikaci