Porous graphitic chromatography in urine analysis

a 2021

Porous graphitic chromatography in urine analysis

ŠMAK, Pavel, Jana GREGOROVÁ, Lucie NOVOTNÁ, Lenka KUBINYIOVÁ, Ondřej PEŠ et. al.

Základní údaje

Originální název

Porous graphitic chromatography in urine analysis

Autoři

ŠMAK, Pavel (203 Česká republika, domácí), Jana GREGOROVÁ (203 Česká republika, domácí), Lucie NOVOTNÁ (203 Česká republika, domácí), Lenka KUBINYIOVÁ (203 Česká republika, domácí) a Ondřej PEŠ (203 Česká republika, garant, domácí)

Vydání

22. ročník Školy hmotnostní spektrometrie, 2021

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10609 Biochemical research methods

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14110/21:00122329

Organizační jednotka

Lékařská fakulta

ISBN

978-80-88195-25-2

Klíčová slova anglicky

urine; chromatography; mass spectrometry; porous graphitic carbon

Změněno: 14. 9. 2021 10:04, doc. Mgr. Ondřej Peš, Ph.D.

Anotace

V originále

Urine is rich in the products of metabolism, some of which are linked to different conditions, such as inflammation or metabolic disorders. The traditional analytical procedure involving reversed-phase liquid chromatography (LC) is unsuitable for analysis of polar compounds unless ion pair reagents are used. Hydrophilic interaction chromatography often requires high ionic strength to overcome the broad tailing of basic compounds and is limited to pH < 8. Porous graphitic chromatography (PGC) in connection to mass spectrometry (MS) may encompass analysis of polar and nonpolar compounds at the same time using a broad range of conditions such as pH and ionic strength of MS compatible buffers and additives. Reliable quantitation of analytes in urine demands knowing either daily diuresis or the concentration of creatinine, which is often done separately (e.g., Jaffe's reaction, or direct injection analysis). Here we aimed to establish the background for the development of routine methods for urine compounds separation and quantitation. Selected compounds from the class of catecholamines and creatinine have been analyzed using LC MS on a PGC column and various conditions affecting the final separation have been tested. The ability to quantify selected analytes and creatinine in a single run could significantly simplify the analytical process (and potentially lower the costs of the analyses). Nevertheless, such a method is not currently available for routine analyses in clinical practice.

Návaznosti

MUNI/A/1266/2020, interní kód MU

Název: Příspěvek [bio]chemických metod při studiu molekulární podstaty vybraných patologických jevů a onemocnění

Investor: Masarykova univerzita, Příspěvek [bio]chemických metod při studiu molekulární podstaty vybraných patologických jevů a onemocnění

Citovat

ŠMAK, Pavel, Jana GREGOROVÁ, Lucie NOVOTNÁ, Lenka KUBINYIOVÁ a Ondřej PEŠ. Porous graphitic chromatography in urine analysis. In 22. ročník Školy hmotnostní spektrometrie. 2021. ISBN 978-80-88195-25-2.

@proceedings{1791755,
   author = {Šmak, Pavel and Gregorová, Jana and Novotná, Lucie and Kubinyiová, Lenka and Peš, Ondřej},
   booktitle = {22. ročník Školy hmotnostní spektrometrie},
   keywords = {urine; chromatography; mass spectrometry; porous graphitic carbon},
   language = {eng},
   isbn = {978-80-88195-25-2},
   title = {Porous graphitic chromatography in urine analysis},
   url = {http://skolams2021.spektroskopie.cz/},
   year = {2021}
}

TY  - CONF
ID  - 1791755
AU  - Šmak, Pavel - Gregorová, Jana - Novotná, Lucie - Kubinyiová, Lenka - Peš, Ondřej
PY  - 2021
TI  - Porous graphitic chromatography in urine analysis
SN  - 9788088195252
KW  - urine
KW  - chromatography
KW  - mass spectrometry
KW  - porous graphitic carbon
UR  - http://skolams2021.spektroskopie.cz/
N2  - Urine is rich in the products of metabolism, some of which are linked to different conditions, such as inflammation or metabolic disorders. The traditional analytical procedure involving reversed-phase liquid chromatography (LC) is unsuitable for analysis of polar compounds unless ion pair reagents are used. Hydrophilic interaction chromatography often requires high ionic strength to overcome the broad tailing of basic compounds and is limited to pH < 8. Porous graphitic chromatography (PGC) in connection to mass spectrometry (MS) may encompass analysis of polar and nonpolar compounds at the same time using a broad range of conditions such as pH and ionic strength of MS compatible buffers and additives. Reliable quantitation of analytes in urine demands knowing either daily diuresis or the concentration of creatinine, which is often done separately (e.g., Jaffe's reaction, or direct injection analysis). Here we aimed to establish the background for the development of routine methods for urine compounds separation and quantitation. Selected compounds from the class of catecholamines and creatinine have been analyzed using LC MS on a PGC column and various conditions affecting the final separation have been tested. The ability to quantify selected analytes and creatinine in a single run could significantly simplify the analytical process (and potentially lower the costs of the analyses). Nevertheless, such a method is not currently available for routine analyses in clinical practice.
ER  -

ŠMAK, Pavel, Jana GREGOROVÁ, Lucie NOVOTNÁ, Lenka KUBINYIOVÁ a Ondřej PEŠ. Porous graphitic chromatography in urine analysis. In \textit{22. ročník Školy hmotnostní spektrometrie}. 2021. ISBN~978-80-88195-25-2.

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