ŠMAK, Pavel, Jana GREGOROVÁ, Lucie NOVOTNÁ, Lenka KUBINYIOVÁ and Ondřej PEŠ. Porous graphitic chromatography in urine analysis. In 22. ročník Školy hmotnostní spektrometrie. 2021. ISBN 978-80-88195-25-2.
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Basic information
Original name Porous graphitic chromatography in urine analysis
Authors ŠMAK, Pavel (203 Czech Republic, belonging to the institution), Jana GREGOROVÁ (203 Czech Republic, belonging to the institution), Lucie NOVOTNÁ (203 Czech Republic, belonging to the institution), Lenka KUBINYIOVÁ (203 Czech Republic, belonging to the institution) and Ondřej PEŠ (203 Czech Republic, guarantor, belonging to the institution).
Edition 22. ročník Školy hmotnostní spektrometrie, 2021.
Other information
Original language English
Type of outcome Conference abstract
Field of Study 10609 Biochemical research methods
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
WWW URL
RIV identification code RIV/00216224:14110/21:00122329
Organization unit Faculty of Medicine
ISBN 978-80-88195-25-2
Keywords in English urine; chromatography; mass spectrometry; porous graphitic carbon
Changed by Changed by: doc. Mgr. Ondřej Peš, Ph.D., učo 63698. Changed: 14/9/2021 10:04.
Abstract
Urine is rich in the products of metabolism, some of which are linked to different conditions, such as inflammation or metabolic disorders. The traditional analytical procedure involving reversed-phase liquid chromatography (LC) is unsuitable for analysis of polar compounds unless ion pair reagents are used. Hydrophilic interaction chromatography often requires high ionic strength to overcome the broad tailing of basic compounds and is limited to pH < 8. Porous graphitic chromatography (PGC) in connection to mass spectrometry (MS) may encompass analysis of polar and nonpolar compounds at the same time using a broad range of conditions such as pH and ionic strength of MS compatible buffers and additives. Reliable quantitation of analytes in urine demands knowing either daily diuresis or the concentration of creatinine, which is often done separately (e.g., Jaffe's reaction, or direct injection analysis). Here we aimed to establish the background for the development of routine methods for urine compounds separation and quantitation. Selected compounds from the class of catecholamines and creatinine have been analyzed using LC MS on a PGC column and various conditions affecting the final separation have been tested. The ability to quantify selected analytes and creatinine in a single run could significantly simplify the analytical process (and potentially lower the costs of the analyses). Nevertheless, such a method is not currently available for routine analyses in clinical practice.
Links
MUNI/A/1266/2020, interní kód MUName: Příspěvek [bio]chemických metod při studiu molekulární podstaty vybraných patologických jevů a onemocnění
Investor: Masaryk University
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