Necessity of flow cytometry assessment of circulating plasma
cells and its connection with clinical characteristics of
primary and secondary plasma cell leukaemia

J 2021

Necessity of flow cytometry assessment of circulating plasma cells and its connection with clinical characteristics of primary and secondary plasma cell leukaemia

BEZDĚKOVÁ, Renata, Tomas JELINEK, Romana KRÁLOVÁ, Martin ŠTORK, Petra POLACKOVA et. al.

Základní údaje

Originální název

Necessity of flow cytometry assessment of circulating plasma cells and its connection with clinical characteristics of primary and secondary plasma cell leukaemia

Autoři

BEZDĚKOVÁ, Renata (203 Česká republika, domácí), Tomas JELINEK (203 Česká republika), Romana KRÁLOVÁ (203 Česká republika, domácí), Martin ŠTORK (203 Česká republika), Petra POLACKOVA (203 Česká republika), Pavla VŠIANSKÁ (203 Česká republika, domácí), Lucie KUBÍNOVÁ (203 Česká republika, domácí), Jiří JARKOVSKÝ (203 Česká republika, domácí), Martina ALMÁŠI (203 Česká republika, domácí), Ivanna BOICHUK (804 Ukrajina), Zdenka KNECHTOVA (203 Česká republika), Miroslav PENKA (203 Česká republika), Luděk POUR (203 Česká republika), Sabina ŠEVČÍKOVÁ (203 Česká republika, domácí), Roman HAJEK (203 Česká republika) a Lucie ŘÍHOVÁ (203 Česká republika, garant, domácí)

Vydání

British journal of haematology, England, Wiley-Blackwell, 2021, 0007-1048

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30205 Hematology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 8.615

Kód RIV

RIV/00216224:14110/21:00122390

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1111/bjh.17713

UT WoS

000694648500001

Klíčová slova anglicky

plasma cell leukaemia; multiple myeloma; plasma cell; flow cytometry; prognosis; phenotype

Štítky

14110518, 14119612, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 7. 12. 2021 12:52, Mgr. Tereza Miškechová

Anotace

V originále

Plasma cell leukaemia (PCL) is a rare and very aggressive plasma cell disorder. Preventing a dismal outcome of PCL requires early diagnosis with appropriate analytical tools. Therefore, the investigation of 33 patients with primary and secondary PCL was done when the quantity of circulating plasma cells (PCs) using flow cytometry (FC) and morphology assessment was evaluated. The phenotypic profile of the PCs was also analysed to determine if there is an association with clinical outcomes and to evaluate the prognostic value of analysed markers. Our results revealed that FC is an excellent method for identifying circulating PCs as a significantly higher number was identified by FC than by morphology (26 center dot 7% vs. 13 center dot 5%, P = 0 center dot 02). None of secondary PCL cases expressed CD19 or CD20. A low level of expression with similar positivity of CD27, CD28, CD81 and CD117 was found in both PCL groups. A decrease of CD44 expression was detected only in secondary PCL. Expression of CD56 was present in more than half of PCL cases as well as cytoplasmic nestin. A decreased level of platelets, Eastern Cooperative Oncology Group score of 2-3 and lack of CD20(+) PC were associated with a higher risk of death. FC could be incorporated in PCL diagnostics not only to determine the number of circulating PCs, but also to assess their phenotype profile and this information should be useful in patients' diagnosis and possible prognosis.

Citovat

BEZDĚKOVÁ, Renata, Tomas JELINEK, Romana KRÁLOVÁ, Martin ŠTORK, Petra POLACKOVA, Pavla VŠIANSKÁ, Lucie KUBÍNOVÁ, Jiří JARKOVSKÝ, Martina ALMÁŠI, Ivanna BOICHUK, Zdenka KNECHTOVA, Miroslav PENKA, Luděk POUR, Sabina ŠEVČÍKOVÁ, Roman HAJEK a Lucie ŘÍHOVÁ. Necessity of flow cytometry assessment of circulating plasma cells and its connection with clinical characteristics of primary and secondary plasma cell leukaemia. British journal of haematology. England: Wiley-Blackwell, 2021, roč. 195, č. 1, s. 95-107. ISSN 0007-1048. Dostupné z: https://dx.doi.org/10.1111/bjh.17713.

@article{1793183,
   author = {Bezděková, Renata and Jelinek, Tomas and Králová, Romana and Štork, Martin and Polackova, Petra and Všianská, Pavla and Kubínová, Lucie and Jarkovský, Jiří and Almáši, Martina and Boichuk, Ivanna and Knechtova, Zdenka and Penka, Miroslav and Pour, Luděk and Ševčíková, Sabina and Hajek, Roman and Říhová, Lucie},
   article_location = {England},
   article_number = {1},
   doi = {http://dx.doi.org/10.1111/bjh.17713},
   keywords = {plasma cell leukaemia; multiple myeloma; plasma cell; flow cytometry; prognosis; phenotype},
   language = {eng},
   issn = {0007-1048},
   journal = {British journal of haematology},
   title = {Necessity of flow cytometry assessment of circulating plasma cells and its connection with clinical characteristics of primary and secondary plasma cell leukaemia},
   url = {https://onlinelibrary.wiley.com/doi/10.1111/bjh.17713},
   volume = {195},
   year = {2021}
}

TY  - JOUR
ID  - 1793183
AU  - Bezděková, Renata - Jelinek, Tomas - Králová, Romana - Štork, Martin - Polackova, Petra - Všianská, Pavla - Kubínová, Lucie - Jarkovský, Jiří - Almáši, Martina - Boichuk, Ivanna - Knechtova, Zdenka - Penka, Miroslav - Pour, Luděk - Ševčíková, Sabina - Hajek, Roman - Říhová, Lucie
PY  - 2021
TI  - Necessity of flow cytometry assessment of circulating plasma cells and its connection with clinical characteristics of primary and secondary plasma cell leukaemia
JF  - British journal of haematology
VL  - 195
IS  - 1
SP  - 95-107
EP  - 95-107
PB  - Wiley-Blackwell
SN  - 00071048
KW  - plasma cell leukaemia
KW  - multiple myeloma
KW  - plasma cell
KW  - flow cytometry
KW  - prognosis
KW  - phenotype
UR  - https://onlinelibrary.wiley.com/doi/10.1111/bjh.17713
N2  - Plasma cell leukaemia (PCL) is a rare and very aggressive plasma cell disorder. Preventing a dismal outcome of PCL requires early diagnosis with appropriate analytical tools. Therefore, the investigation of 33 patients with primary and secondary PCL was done when the quantity of circulating plasma cells (PCs) using flow cytometry (FC) and morphology assessment was evaluated. The phenotypic profile of the PCs was also analysed to determine if there is an association with clinical outcomes and to evaluate the prognostic value of analysed markers. Our results revealed that FC is an excellent method for identifying circulating PCs as a significantly higher number was identified by FC than by morphology (26 center dot 7% vs. 13 center dot 5%, P = 0 center dot 02). None of secondary PCL cases expressed CD19 or CD20. A low level of expression with similar positivity of CD27, CD28, CD81 and CD117 was found in both PCL groups. A decrease of CD44 expression was detected only in secondary PCL. Expression of CD56 was present in more than half of PCL cases as well as cytoplasmic nestin. A decreased level of platelets, Eastern Cooperative Oncology Group score of 2-3 and lack of CD20(+) PC were associated with a higher risk of death. FC could be incorporated in PCL diagnostics not only to determine the number of circulating PCs, but also to assess their phenotype profile and this information should be useful in patients' diagnosis and possible prognosis.
ER  -

BEZDĚKOVÁ, Renata, Tomas JELINEK, Romana KRÁLOVÁ, Martin ŠTORK, Petra POLACKOVA, Pavla VŠIANSKÁ, Lucie KUBÍNOVÁ, Jiří JARKOVSKÝ, Martina ALMÁŠI, Ivanna BOICHUK, Zdenka KNECHTOVA, Miroslav PENKA, Luděk POUR, Sabina ŠEVČÍKOVÁ, Roman HAJEK a Lucie ŘÍHOVÁ. Necessity of flow cytometry assessment of circulating plasma cells and its connection with clinical characteristics of primary and secondary plasma cell leukaemia. \textit{British journal of haematology}. England: Wiley-Blackwell, 2021, roč.~195, č.~1, s.~95-107. ISSN~0007-1048. Dostupné z: https://dx.doi.org/10.1111/bjh.17713.

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