Contrasting Host-Parasite Population Structure: Morphology and
Mitogenomics of a Parasitic Flatworm on Pelagic Deepwater
Cichlid Fishes from Lake Tanganyika

J 2021

Contrasting Host-Parasite Population Structure: Morphology and Mitogenomics of a Parasitic Flatworm on Pelagic Deepwater Cichlid Fishes from Lake Tanganyika

KMENTOVÁ, Nikol, Christoph HAHN, Stephan KOBLMÜLLER, Holger ZIMMERMANN, Jiří VOREL et. al.

Základní údaje

Originální název

Contrasting Host-Parasite Population Structure: Morphology and Mitogenomics of a Parasitic Flatworm on Pelagic Deepwater Cichlid Fishes from Lake Tanganyika

Autoři

KMENTOVÁ, Nikol (203 Česká republika, garant, domácí), Christoph HAHN (40 Rakousko), Stephan KOBLMÜLLER (40 Rakousko), Holger ZIMMERMANN (40 Rakousko), Jiří VOREL (203 Česká republika, domácí), Tom ARTOIS (56 Belgie), Milan GELNAR (203 Česká republika, domácí) a Maarten Pieterjan VANHOVE (56 Belgie, domácí)

Vydání

Biology, BASEL, MDPI, 2021, 2079-7737

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Article

Impakt faktor

Impact factor: 5.168

Kód RIV

RIV/00216224:14310/21:00119223

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.3390/biology10080797

UT WoS

000688971100001

Klíčová slova anglicky

monogenea; Cichlidogyrus; Bathybatini; cox1; PoolSeq

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 3. 2022 09:49, Mgr. Marie Šípková, DiS.

Anotace

V originále

Simple Summary Species richness in open water areas is generally lower than in coastal zones. Fish parasites have been targeted as biological tags potentially magnifying biological patterns of their hosts including long-distance migrations notoriously difficult to trace. Lake Tanganyika (Africa) is an ideal place to study general mechanisms of host-parasite interactions in an open water environment and Cichlidogyrus, a monogenean flatworm lineage also present in Lake Tanganyika, has been proposed as a model system to study parasite-host relationships. The present study revealed the lake-wide occurrence (600 km) of Cichlidogyrus casuarinus, a parasite with a broad host range infecting pelagic fishes endemic to Lake Tanganyika. Our comparative approach highlighted incongruence between morphological and genetic differentiation of the populations of Cichlidogyrus casuarinus. Our results show a limitation of the parasite's magnifying potential for the focal host species due to the parasites' broad host range including highly mobile host species. Using different sequencing technologies, the study further provides the first assessment of the genetic variation of mitochondrial data in Cichlidogyrus showing contrasting patterns within and between parasite species. Given the now considerable baseline knowledge on its morphological and genetic variation, we propose C. casuarinus as a model to study (1) mechanisms driving host range and (2) the role of phenotypic plasticity in diversification and speciation. Little phylogeographic structure is presumed for highly mobile species in pelagic zones. Lake Tanganyika is a unique ecosystem with a speciose and largely endemic fauna famous for its remarkable evolutionary history. In bathybatine cichlid fishes, the pattern of lake-wide population differentiation differs among species. We assessed the congruence between the phylogeographic structure of bathybatine cichlids and their parasitic flatworm Cichlidogyrus casuarinus to test the magnifying glass hypothesis. Additionally, we evaluated the use of a PoolSeq approach to study intraspecific variation in dactylogyrid monogeneans. The lake-wide population structure of C. casuarinus ex Hemibates stenosoma was assessed based on a portion of the cox1 gene combined with morphological characterisation. Additionally, intraspecific mitogenomic variation among 80 parasite samples from one spatially constrained metapopulation was assessed using shotgun NGS. While no clear geographic genetic structure was detected in parasites, both geographic and host-related phenotypic variation was apparent. The incongruence with the genetic north-south gradient observed in H. stenosoma may be explained by the broad host range of this flatworm including eupelagic bathybatine host species that form panmictic populations across the lake. In addition, we present the first parasite mitogenome from Lake Tanganyika and propose a methodological framework for studying the intraspecific mitogenomic variation of dactylogyrid monogeneans.

Návaznosti

GA19-13573S, projekt VaV

Název: Parazité jako ukazatelé populační struktury sleďovitých ryb jezera Tanganika (Afrika): srovnání genomických dat parazita a hostitele

Investor: Grantová agentura ČR, Parazité jako ukazatelé populační struktury sleďovitých ryb jezera Tanganika (Afrika): srovnání genomických dat parazita a hostitele

GBP505/12/G112, projekt VaV

Název: ECIP - Evropské centrum ichtyoparazitologie

Investor: Grantová agentura ČR, ECIP - Evropské centrum ichtyoparazitologie

LM2018140, projekt VaV

Název: e-Infrastruktura CZ (Akronym: e-INFRA CZ)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, e-Infrastruktura CZ

Citovat

KMENTOVÁ, Nikol, Christoph HAHN, Stephan KOBLMÜLLER, Holger ZIMMERMANN, Jiří VOREL, Tom ARTOIS, Milan GELNAR a Maarten Pieterjan VANHOVE. Contrasting Host-Parasite Population Structure: Morphology and Mitogenomics of a Parasitic Flatworm on Pelagic Deepwater Cichlid Fishes from Lake Tanganyika. Biology. BASEL: MDPI, 2021, roč. 10, č. 8, s. "797", 24 s. ISSN 2079-7737. Dostupné z: https://dx.doi.org/10.3390/biology10080797.

@article{1793219,
   author = {Kmentová, Nikol and Hahn, Christoph and Koblmüller, Stephan and Zimmermann, Holger and Vorel, Jiří and Artois, Tom and Gelnar, Milan and Vanhove, Maarten Pieterjan},
   article_location = {BASEL},
   article_number = {8},
   doi = {http://dx.doi.org/10.3390/biology10080797},
   keywords = {monogenea; Cichlidogyrus; Bathybatini; cox1; PoolSeq},
   language = {eng},
   issn = {2079-7737},
   journal = {Biology},
   title = {Contrasting Host-Parasite Population Structure: Morphology and Mitogenomics of a Parasitic Flatworm on Pelagic Deepwater Cichlid Fishes from Lake Tanganyika},
   url = {https://doi.org/10.3390/biology10080797},
   volume = {10},
   year = {2021}
}

TY  - JOUR
ID  - 1793219
AU  - Kmentová, Nikol - Hahn, Christoph - Koblmüller, Stephan - Zimmermann, Holger - Vorel, Jiří - Artois, Tom - Gelnar, Milan - Vanhove, Maarten Pieterjan
PY  - 2021
TI  - Contrasting Host-Parasite Population Structure: Morphology and Mitogenomics of a Parasitic Flatworm on Pelagic Deepwater Cichlid Fishes from Lake Tanganyika
JF  - Biology
VL  - 10
IS  - 8
SP  - "797"
EP  - "797"
PB  - MDPI
SN  - 20797737
KW  - monogenea
KW  - Cichlidogyrus
KW  - Bathybatini
KW  - cox1
KW  - PoolSeq
UR  - https://doi.org/10.3390/biology10080797
N2  - Simple Summary Species richness in open water areas is generally lower than in coastal zones. Fish parasites have been targeted as biological tags potentially magnifying biological patterns of their hosts including long-distance migrations notoriously difficult to trace. Lake Tanganyika (Africa) is an ideal place to study general mechanisms of host-parasite interactions in an open water environment and Cichlidogyrus, a monogenean flatworm lineage also present in Lake Tanganyika, has been proposed as a model system to study parasite-host relationships. The present study revealed the lake-wide occurrence (600 km) of Cichlidogyrus casuarinus, a parasite with a broad host range infecting pelagic fishes endemic to Lake Tanganyika. Our comparative approach highlighted incongruence between morphological and genetic differentiation of the populations of Cichlidogyrus casuarinus. Our results show a limitation of the parasite's magnifying potential for the focal host species due to the parasites' broad host range including highly mobile host species. Using different sequencing technologies, the study further provides the first assessment of the genetic variation of mitochondrial data in Cichlidogyrus showing contrasting patterns within and between parasite species. Given the now considerable baseline knowledge on its morphological and genetic variation, we propose C. casuarinus as a model to study (1) mechanisms driving host range and (2) the role of phenotypic plasticity in diversification and speciation. Little phylogeographic structure is presumed for highly mobile species in pelagic zones. Lake Tanganyika is a unique ecosystem with a speciose and largely endemic fauna famous for its remarkable evolutionary history. In bathybatine cichlid fishes, the pattern of lake-wide population differentiation differs among species. We assessed the congruence between the phylogeographic structure of bathybatine cichlids and their parasitic flatworm Cichlidogyrus casuarinus to test the magnifying glass hypothesis. Additionally, we evaluated the use of a PoolSeq approach to study intraspecific variation in dactylogyrid monogeneans. The lake-wide population structure of C. casuarinus ex Hemibates stenosoma was assessed based on a portion of the cox1 gene combined with morphological characterisation. Additionally, intraspecific mitogenomic variation among 80 parasite samples from one spatially constrained metapopulation was assessed using shotgun NGS. While no clear geographic genetic structure was detected in parasites, both geographic and host-related phenotypic variation was apparent. The incongruence with the genetic north-south gradient observed in H. stenosoma may be explained by the broad host range of this flatworm including eupelagic bathybatine host species that form panmictic populations across the lake. In addition, we present the first parasite mitogenome from Lake Tanganyika and propose a methodological framework for studying the intraspecific mitogenomic variation of dactylogyrid monogeneans.
ER  -

KMENTOVÁ, Nikol, Christoph HAHN, Stephan KOBLMÜLLER, Holger ZIMMERMANN, Jiří VOREL, Tom ARTOIS, Milan GELNAR a Maarten Pieterjan VANHOVE. Contrasting Host-Parasite Population Structure: Morphology and Mitogenomics of a Parasitic Flatworm on Pelagic Deepwater Cichlid Fishes from Lake Tanganyika. \textit{Biology}. BASEL: MDPI, 2021, roč.~10, č.~8, s.~''797'', 24 s. ISSN~2079-7737. Dostupné z: https://dx.doi.org/10.3390/biology10080797.

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