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@article{1793697,
author = {Wang, Z.Q. and Pisano, S. and Ghini, V. and Kadeřávek, Pavel and Zachrdla, M. and Pelupessy, P. and Kazmierczak, M. and Marquardsen, T. and Tyburn, J.M. and Bouvignies, G. and Parigi, G. and Luchinat, C. and Ferrage, F.},
article_location = {Washington},
article_number = {25},
doi = {http://dx.doi.org/10.1021/jacs.1c01388},
keywords = {NUCLEAR-MAGNETIC-RESONANCETRANSFER DIFFERENCE NMRHUMAN SERUM-ALBUMINLIGAND-BINDINGH-1-NMR SPECTROSCOPYLOW-FIELDP-31 NMRRELAXATIONMETABOLOMICSMETABONOMICS},
language = {eng},
issn = {0002-7863},
journal = {Journal of the American Chemical Society},
title = {Detection of Metabolite-Protein Interactions in Complex Biological Samples by High-Resolution Relaxometry: Toward Interactomics by NMR},
url = {https://pubs.acs.org/doi/10.1021/jacs.1c01388},
volume = {143},
year = {2021}
}
TY - JOUR
ID - 1793697
AU - Wang, Z.Q. - Pisano, S. - Ghini, V. - Kadeřávek, Pavel - Zachrdla, M. - Pelupessy, P. - Kazmierczak, M. - Marquardsen, T. - Tyburn, J.M. - Bouvignies, G. - Parigi, G. - Luchinat, C. - Ferrage, F.
PY - 2021
TI - Detection of Metabolite-Protein Interactions in Complex Biological Samples by High-Resolution Relaxometry: Toward Interactomics by NMR
JF - Journal of the American Chemical Society
VL - 143
IS - 25
SP - 9393-9404
EP - 9393-9404
PB - American Chemical Society
SN - 00027863
KW - NUCLEAR-MAGNETIC-RESONANCETRANSFER DIFFERENCE NMRHUMAN SERUM-ALBUMINLIGAND-BINDINGH-1-NMR SPECTROSCOPYLOW-FIELDP-31 NMRRELAXATIONMETABOLOMICSMETABONOMICS
UR - https://pubs.acs.org/doi/10.1021/jacs.1c01388
N2 - Metabolomics, the systematic investigation of metabolites in biological fluids, cells, or tissues, reveals essential information about metabolism and diseases. Metabolites have functional roles in a myriad of biological processes, as substrates and products of enzymatic reactions but also as cofactors and regulators of large numbers of biochemical mechanisms. These functions involve interactions of metabolites with macromolecules. Yet, methods to systematically investigate these interactions are still scarce to date. In particular, there is a need for techniques suited to identify and characterize weak metabolite-macromolecule interactions directly in complex media such as biological fluids. Here, we introduce a method to investigate weak interactions between metabolites and macromolecules in biological fluids. Our approach is based on high-resolution NMR relaxometry and does not require any invasive procedure or separation step. We show that we can detect interactions between small and large molecules in human blood serum and quantify the size of the complex. Our work opens the way for investigations of metabolite (or other small molecules)-protein interactions in biological fluids for interactomics or pharmaceutical applications.
ER -
WANG, Z.Q., S. PISANO, V. GHINI, Pavel KADEŘÁVEK, M. ZACHRDLA, P. PELUPESSY, M. KAZMIERCZAK, T. MARQUARDSEN, J.M. TYBURN, G. BOUVIGNIES, G. PARIGI, C. LUCHINAT a F. FERRAGE. Detection of Metabolite-Protein Interactions in Complex Biological Samples by High-Resolution Relaxometry: Toward Interactomics by NMR. \textit{Journal of the American Chemical Society}. Washington: American Chemical Society, 2021, roč.~143, č.~25, s.~9393-9404. ISSN~0002-7863. Dostupné z: https://dx.doi.org/10.1021/jacs.1c01388.
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