Effect of Particle Size and Surface Chemistry of Photon-Upconversion Nanoparticles on Analog and Digital Immunoassays for Cardiac Troponin

BRANDMEIER, Julian, Kirsti RAIKO, Zdeněk FARKA, Riikka PELTOMAA, Matthias Jürgen MICKERT, Antonín HLAVÁČEK, Petr SKLÁDAL, Tero SOUKKA and Hans-Heiner GORRIS. Effect of Particle Size and Surface Chemistry of Photon-Upconversion Nanoparticles on Analog and Digital Immunoassays for Cardiac Troponin. Advanced Healthcare Materials. Wiley-VCH, 2021, vol. 10, No 18, p. 1-9. ISSN 2192-2640. Available from: https://dx.doi.org/10.1002/adhm.202100506.

Basic information

• Original name: Effect of Particle Size and Surface Chemistry of Photon-Upconversion Nanoparticles on Analog and Digital Immunoassays for Cardiac Troponin
• Authors: BRANDMEIER, Julian (276 Germany), Kirsti RAIKO (246 Finland), Zdeněk FARKA (203 Czech Republic, guarantor, belonging to the institution), Riikka PELTOMAA (246 Finland), Matthias Jürgen MICKERT (276 Germany), Antonín HLAVÁČEK (203 Czech Republic), Petr SKLÁDAL (203 Czech Republic, belonging to the institution), Tero SOUKKA and Hans-Heiner GORRIS (276 Germany).
• Edition: Advanced Healthcare Materials, Wiley-VCH, 2021, 2192-2640.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10406 Analytical chemistry
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 11.092
• RIV identification code: RIV/00216224:14310/21:00119240
• Organization unit: Faculty of Science
• Doi: http://dx.doi.org/10.1002/adhm.202100506
• UT WoS: 000673454600001
• Keywords in English: anti-Stokes emission; cardiac arrest; lanthadine-dopoed nanomaterials; single-molecule immunoassay; troponin; photon-upconversion; upconversion-linked immunosorbent assay
• Tags: CF CRYO, CF NANO, rivok
• Tags: International impact, Reviewed

Abstract

Sensitive immunoassays are required for troponin, a low-abundance cardiac biomarker in blood. In contrast to conventional (analog) assays that measure the integrated signal of thousands of molecules, digital assays are based on counting individual biomarker molecules. Photon-upconversion nanoparticles (UCNP) are an excellent nanomaterial for labeling and detecting single biomarker molecules because their unique anti-Stokes emission avoids optical interference, and single nanoparticles can be reliably distinguished from the background signal. Here, the effect of the surface architecture and size of UCNP labels on the performance of upconversion-linked immunosorbent assays (ULISA) is critically assessed. The size, brightness, and surface architecture of UCNP labels are more important for measuring low troponin concentrations in human plasma than changing from an analog to a digital detection mode. Both detection modes result approximately in the same assay sensitivity, reaching a limit of detection (LOD) of 10 pg mL−1 in plasma, which is in the range of troponin concentrations found in the blood of healthy individuals.

Links

• GA21-03156S, research and development project: Name: Foton-upkonverzní značky pro mikrofluidní jednomolekulové imunostanovení proteinových biomarkerů

Investor: Czech Science Foundation

• LM2018127, research and development project: Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

• LQ1601, research and development project: Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

• LTAB19011, research and development project: Name: Luminiscenční imunostanovení jako citlivý nástroj pro diagnostiku nemocí včel

Investor: Ministry of Education, Youth and Sports of the CR, Sensitive bioanalytical tools for the surveillance of honey bee diseases, INTER-ACTION