2021
3D Bioprinting of Engineered Tissue Flaps with Hierarchical Vessel Networks (VesselNet) for Direct Host-To-Implant Perfusion
SZKLANNY, Ariel A., Majd MACHOUR, Idan REDENSKI, Václav CHOCHOLA, Idit GOLDFRACHT et. al.Základní údaje
Originální název
3D Bioprinting of Engineered Tissue Flaps with Hierarchical Vessel Networks (VesselNet) for Direct Host-To-Implant Perfusion
Autoři
SZKLANNY, Ariel A., Majd MACHOUR, Idan REDENSKI, Václav CHOCHOLA (203 Česká republika, domácí), Idit GOLDFRACHT, Ben KAPLAN, Mark EPSHTEIN, Haneen Simaan YAMEEN, Uri MERDLER, Adam FEINBERG, Dror SELIKTAR, Netanel KORIN, Josef JAROŠ (203 Česká republika) a Shulamit LEVENBERG (garant)
Vydání
ADVANCED MATERIALS, WEINHEIM, WILEY-V C H VERLAG GMBH, 2021, 0935-9648
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
30201 Cardiac and Cardiovascular systems
Stát vydavatele
Německo
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Impakt faktor
Impact factor: 32.086
Kód RIV
RIV/00216224:14110/21:00122520
Organizační jednotka
Lékařská fakulta
UT WoS
000696041200001
Klíčová slova anglicky
3D bioprinting; ECM bioink; engineered flap; personalized medicine; tissue engineering; vascularization.
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 15. 2. 2022 11:29, Mgr. Tereza Miškechová
Anotace
V originále
Engineering hierarchical vasculatures is critical for creating implantable functional thick tissues. Current approaches focus on fabricating mesoscale vessels for implantation or hierarchical microvascular in vitro models, but a combined approach is yet to be achieved to create engineered tissue flaps. Here, millimetric vessel-like scaffolds and 3D bioprinted vascularized tissues interconnect, creating fully engineered hierarchical vascular constructs for implantation. Endothelial and support cells spontaneously form microvascular networks in bioprinted tissues using a human collagen bioink. Sacrificial molds are used to create polymeric vessel-like scaffolds and endothelial cells seeded in their lumen form native-like endothelia. Assembling endothelialized scaffolds within vascularizing hydrogels incites the bioprinted vasculature and endothelium to cooperatively create vessels, enabling tissue perfusion through the scaffold lumen. Using a cuffing microsurgery approach, the engineered tissue is directly anastomosed with a rat femoral artery, promoting a rich host vasculature within the implanted tissue. After two weeks in vivo, contrast microcomputer tomography imaging and lectin perfusion of explanted engineered tissues verify the host ingrowth vasculature's functionality. Furthermore, the hierarchical vessel network (VesselNet) supports in vitro functionality of cardiomyocytes. Finally, the proposed approach is expanded to mimic complex structures with native-like millimetric vessels. This work presents a novel strategy aiming to create fully-engineered patient-specific thick tissue flaps.