J 2021

3D Bioprinting of Engineered Tissue Flaps with Hierarchical Vessel Networks (VesselNet) for Direct Host-To-Implant Perfusion

SZKLANNY, Ariel A., Majd MACHOUR, Idan REDENSKI, Václav CHOCHOLA, Idit GOLDFRACHT et. al.

Základní údaje

Originální název

3D Bioprinting of Engineered Tissue Flaps with Hierarchical Vessel Networks (VesselNet) for Direct Host-To-Implant Perfusion

Autoři

SZKLANNY, Ariel A., Majd MACHOUR, Idan REDENSKI, Václav CHOCHOLA (203 Česká republika, domácí), Idit GOLDFRACHT, Ben KAPLAN, Mark EPSHTEIN, Haneen Simaan YAMEEN, Uri MERDLER, Adam FEINBERG, Dror SELIKTAR, Netanel KORIN, Josef JAROŠ (203 Česká republika) a Shulamit LEVENBERG (garant)

Vydání

ADVANCED MATERIALS, WEINHEIM, WILEY-V C H VERLAG GMBH, 2021, 0935-9648

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30201 Cardiac and Cardiovascular systems

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Impakt faktor

Impact factor: 32.086

Kód RIV

RIV/00216224:14110/21:00122520

Organizační jednotka

Lékařská fakulta

UT WoS

000696041200001

Klíčová slova anglicky

3D bioprinting; ECM bioink; engineered flap; personalized medicine; tissue engineering; vascularization.

Štítky

Příznaky

Mezinárodní význam, Recenzováno
Změněno: 15. 2. 2022 11:29, Mgr. Tereza Miškechová

Anotace

V originále

Engineering hierarchical vasculatures is critical for creating implantable functional thick tissues. Current approaches focus on fabricating mesoscale vessels for implantation or hierarchical microvascular in vitro models, but a combined approach is yet to be achieved to create engineered tissue flaps. Here, millimetric vessel-like scaffolds and 3D bioprinted vascularized tissues interconnect, creating fully engineered hierarchical vascular constructs for implantation. Endothelial and support cells spontaneously form microvascular networks in bioprinted tissues using a human collagen bioink. Sacrificial molds are used to create polymeric vessel-like scaffolds and endothelial cells seeded in their lumen form native-like endothelia. Assembling endothelialized scaffolds within vascularizing hydrogels incites the bioprinted vasculature and endothelium to cooperatively create vessels, enabling tissue perfusion through the scaffold lumen. Using a cuffing microsurgery approach, the engineered tissue is directly anastomosed with a rat femoral artery, promoting a rich host vasculature within the implanted tissue. After two weeks in vivo, contrast microcomputer tomography imaging and lectin perfusion of explanted engineered tissues verify the host ingrowth vasculature's functionality. Furthermore, the hierarchical vessel network (VesselNet) supports in vitro functionality of cardiomyocytes. Finally, the proposed approach is expanded to mimic complex structures with native-like millimetric vessels. This work presents a novel strategy aiming to create fully-engineered patient-specific thick tissue flaps.