Voltammetric studies of selected porphyrin G-quadruplex ligands
and their interaction with DNA in solution and at the mercury
electrode surface

J 2021

Voltammetric studies of selected porphyrin G-quadruplex ligands and their interaction with DNA in solution and at the mercury electrode surface

DOBROVODSKÝ, Daniel, Ales DANHEL, Emmanuelle MOTHES-MARTIN, Genevieve PRATVIEL, Jean-Louis MERGNY et. al.

Základní údaje

Originální název

Voltammetric studies of selected porphyrin G-quadruplex ligands and their interaction with DNA in solution and at the mercury electrode surface

Autoři

DOBROVODSKÝ, Daniel (703 Slovensko, garant, domácí), Ales DANHEL, Emmanuelle MOTHES-MARTIN, Genevieve PRATVIEL, Jean-Louis MERGNY a Miroslav FOJTA

Vydání

Electrochimica Acta, Elsevier, 2021, 0013-4686

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10603 Genetics and heredity

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 7.336

Kód RIV

RIV/00216224:14310/21:00122652

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.electacta.2021.139151

UT WoS

000703171900001

Klíčová slova anglicky

DNA; G-quadruplex; Ligand; Mercury electrode; Porphyrin; Voltammetry

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 25. 10. 2021 10:34, Mgr. Marie Šípková, DiS.

Anotace

V originále

With the increasing interest in DNA G-quadruplexes (G4) as important regulatory elements and prospective therapeutical targets, DNA structure-sensitive electrochemical methods can be advantageously used for studying of their structure or screening for interactions with G4 ligands developed as candidate drugs. Porphyrin derivatives are among the most prominent classes of G4 ligands. Interactions of porphyrins H2-TMPy2PP and Cu-TMPy2PP with oligodeoxynucleotides (ODNs) either forming or not forming G4 were investigated in this work. For this purpose, cyclic voltammetry at hanging mercury drop electrode (HMDE) complemented by UV-vis and CD spectroscopy was applied. Both porphyrins were found to interact strongly with G4 in solution by UV-vis titrations. However, Cu-TMPy2PP appears to interact also with double-stranded and single-stranded ODNs. Accordingly, cyclic voltammetry was able to distinguish between free and ODN-bound forms of Cu-TMPy2PP, but not between binding of the ligand to ODNs with or without G4 propensity.

Citovat

DOBROVODSKÝ, Daniel, Ales DANHEL, Emmanuelle MOTHES-MARTIN, Genevieve PRATVIEL, Jean-Louis MERGNY a Miroslav FOJTA. Voltammetric studies of selected porphyrin G-quadruplex ligands and their interaction with DNA in solution and at the mercury electrode surface. Electrochimica Acta. Elsevier, 2021, roč. 394, October, s. 139151-139161. ISSN 0013-4686. Dostupné z: https://dx.doi.org/10.1016/j.electacta.2021.139151.

@article{1799515,
   author = {Dobrovodský, Daniel and Danhel, Ales and MothesandMartin, Emmanuelle and Pratviel, Genevieve and Mergny, JeanandLouis and Fojta, Miroslav},
   article_number = {October},
   doi = {http://dx.doi.org/10.1016/j.electacta.2021.139151},
   keywords = {DNA; G-quadruplex; Ligand; Mercury electrode; Porphyrin; Voltammetry},
   language = {eng},
   issn = {0013-4686},
   journal = {Electrochimica Acta},
   title = {Voltammetric studies of selected porphyrin G-quadruplex ligands and their interaction with DNA in solution and at the mercury electrode surface},
   url = {https://doi.org/10.1016/j.electacta.2021.139151},
   volume = {394},
   year = {2021}
}

TY  - JOUR
ID  - 1799515
AU  - Dobrovodský, Daniel - Danhel, Ales - Mothes-Martin, Emmanuelle - Pratviel, Genevieve - Mergny, Jean-Louis - Fojta, Miroslav
PY  - 2021
TI  - Voltammetric studies of selected porphyrin G-quadruplex ligands and their interaction with DNA in solution and at the mercury electrode surface
JF  - Electrochimica Acta
VL  - 394
IS  - October
SP  - 139151
EP  - 139151
PB  - Elsevier
SN  - 00134686
KW  - DNA
KW  - G-quadruplex
KW  - Ligand
KW  - Mercury electrode
KW  - Porphyrin
KW  - Voltammetry
UR  - https://doi.org/10.1016/j.electacta.2021.139151
N2  - With the increasing interest in DNA G-quadruplexes (G4) as important regulatory elements and prospective therapeutical targets, DNA structure-sensitive electrochemical methods can be advantageously used for studying of their structure or screening for interactions with G4 ligands developed as candidate drugs. Porphyrin derivatives are among the most prominent classes of G4 ligands. Interactions of porphyrins H2-TMPy2PP and Cu-TMPy2PP with oligodeoxynucleotides (ODNs) either forming or not forming G4 were investigated in this work. For this purpose, cyclic voltammetry at hanging mercury drop electrode (HMDE) complemented by UV-vis and CD spectroscopy was applied. Both porphyrins were found to interact strongly with G4 in solution by UV-vis titrations. However, Cu-TMPy2PP appears to interact also with double-stranded and single-stranded ODNs. Accordingly, cyclic voltammetry was able to distinguish between free and ODN-bound forms of Cu-TMPy2PP, but not between binding of the ligand to ODNs with or without G4 propensity.
ER  -

DOBROVODSKÝ, Daniel, Ales DANHEL, Emmanuelle MOTHES-MARTIN, Genevieve PRATVIEL, Jean-Louis MERGNY a Miroslav FOJTA. Voltammetric studies of selected porphyrin G-quadruplex ligands and their interaction with DNA in solution and at the mercury electrode surface. \textit{Electrochimica Acta}. Elsevier, 2021, roč.~394, October, s.~139151-139161. ISSN~0013-4686. Dostupné z: https://dx.doi.org/10.1016/j.electacta.2021.139151.

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