De novo developed microsatellite markers for monogeneans and
their application to study population genetics of generalist
Dactylogyrus species

a 2021

De novo developed microsatellite markers for monogeneans and their application to study population genetics of generalist Dactylogyrus species

BENOVICS, Michal, Lenka GETTOVÁ a Andrea VETEŠNÍKOVÁ ŠIMKOVÁ

Základní údaje

Originální název

De novo developed microsatellite markers for monogeneans and their application to study population genetics of generalist Dactylogyrus species

Autoři

BENOVICS, Michal (703 Slovensko, domácí), Lenka GETTOVÁ (703 Slovensko, domácí) a Andrea VETEŠNÍKOVÁ ŠIMKOVÁ (203 Česká republika, garant, domácí)

Vydání

26th Helminthological Days, 2021

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/21:00119293

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-906644-9-4

Klíčová slova anglicky

Monogenea; Dactylogyrus; Leuciscidae; microsatellites; phylogeography; genetic diversity

Změněno: 22. 11. 2021 09:11, Mgr. Michal Benovics, Ph.D.

Anotace

V originále

Over the last few decades, genetic markers have been intensively developed to study the population genetics in a wide range of organisms; however, they are weakly applied in parasites' population studies. Microsatellite markers (together with mitochondrial DNA) are considered as a golden standard for population genetic studies. These highly polymorphic short tandem repeats are due to their unique characteristics (e.g., high allelic variance, codominance, and Mendelian inheritance) usually applied to infer gene flow rate, hybridization, or mating patterns on the intraand interpopulation levels. Even though one set of such microsatellite markers was already developed for the monogeneans (more specifically for the Gyrodactylus), they were not previously applied to assess the population structure and diversity of these ectoparasitic helminths. In the present study, we de novo developed set of 24 microsatellite markers and used them to investigate the genetic diversity of the generalist monogenean species Dactylogyrus vistulae parasitizing cyprinoid fish. The analyzed parasite specimens were collected from 13 cyprinoid species from 11 sites in the Apennine and Balkan peninsulas. A total of 159 specimens were genotyped at each of the loci and the number of alleles per locus ranged from 2 to 16, with a mean number of 6.958 alleles per locus. Exceptionally high genetic diversity was observed among D. vistulae individuals in the southern Balkans, suggesting that this region might represent the centre of diversification of Dactylogyrus species in Europe, from where Dactylogyrus parasites expanded to the north of Europe. The initial clustering analysis divided all investigated specimens into three major clusters; however, the results of the subsequent analyses revealed the existence of various subpopulations, suggesting that the population structure of D. vistulae is associated with the diversification of their cyprinoid hosts. In addition, partition of the parasite population was observed in regions of the sympatric occurrence of two host species, indicating that these hosts may represent a barrier to gene flow, even for generalist parasite species.

Návaznosti

GA20-13539S, projekt VaV

Název: Paraziti odhalují historické a součastné kontakty kaprovitých hostitelů: role Blízkého východu v biogeografii západního Palearktu

Investor: Grantová agentura ČR, Paraziti odhalují historické a součastné kontakty kaprovitých hostitelů: role Blízkého východu v biogeografii západního Palearktu

Citovat

BENOVICS, Michal, Lenka GETTOVÁ a Andrea VETEŠNÍKOVÁ ŠIMKOVÁ. De novo developed microsatellite markers for monogeneans and their application to study population genetics of generalist Dactylogyrus species. In 26th Helminthological Days. 2021. ISBN 978-80-906644-9-4.

@proceedings{1799587,
   author = {Benovics, Michal and Gettová, Lenka and Vetešníková Šimková, Andrea},
   booktitle = {26th Helminthological Days},
   keywords = {Monogenea; Dactylogyrus; Leuciscidae; microsatellites; phylogeography; genetic diversity},
   language = {eng},
   isbn = {978-80-906644-9-4},
   title = {De novo developed microsatellite markers for monogeneans and their application to study population genetics of generalist Dactylogyrus species},
   year = {2021}
}

TY  - CONF
ID  - 1799587
AU  - Benovics, Michal - Gettová, Lenka - Vetešníková Šimková, Andrea
PY  - 2021
TI  - De novo developed microsatellite markers for monogeneans and their application to study population genetics of generalist Dactylogyrus species
SN  - 9788090664494
KW  - Monogenea
KW  - Dactylogyrus
KW  - Leuciscidae
KW  - microsatellites
KW  - phylogeography
KW  - genetic diversity
N2  - Over the last few decades, genetic markers have been intensively developed to study the population genetics in a wide range of organisms; however, they are weakly applied in parasites' population studies. Microsatellite markers (together with mitochondrial DNA) are considered as a golden standard for population genetic studies. These highly polymorphic short tandem repeats are due to their unique characteristics (e.g., high allelic variance, codominance, and Mendelian inheritance) usually applied to infer gene flow rate, hybridization, or mating patterns on the intraand interpopulation levels. Even though one set of such microsatellite markers was already developed for the monogeneans (more specifically for the Gyrodactylus), they were not previously applied to assess the population structure and diversity of these ectoparasitic helminths. In the present study, we de novo developed set of 24 microsatellite markers and used them to investigate the genetic diversity of the generalist monogenean species Dactylogyrus vistulae parasitizing cyprinoid fish. The analyzed parasite specimens were collected from 13 cyprinoid species from 11 sites in the Apennine and Balkan peninsulas. A total of 159 specimens were genotyped at each of the loci and the number of alleles per locus ranged from 2 to 16, with a mean number of 6.958 alleles per locus. Exceptionally high genetic diversity was observed among D. vistulae individuals in the southern Balkans, suggesting that this region might represent the centre of diversification of Dactylogyrus species in Europe, from where Dactylogyrus parasites expanded to the north of Europe. The initial clustering analysis divided all investigated specimens into three major clusters; however, the results of the subsequent analyses revealed the existence of various subpopulations, suggesting that the population structure of D. vistulae is associated with the diversification of their cyprinoid hosts. In addition, partition of the parasite population was observed in regions of the sympatric occurrence of two host species, indicating that these hosts may represent a barrier to gene flow, even for generalist parasite species.
ER  -

BENOVICS, Michal, Lenka GETTOVÁ a Andrea VETEŠNÍKOVÁ ŠIMKOVÁ. De novo developed microsatellite markers for monogeneans and their application to study population genetics of generalist Dactylogyrus species. In \textit{26th Helminthological Days}. 2021. ISBN~978-80-906644-9-4.

Podrobný výpis o publikaci