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@article{1799997,
author = {Souralová, Tereza and Holubcová, Zuzana and Kyjovská, Drahomíra and Hampl, Aleš and Koutná, Irena},
article_location = {Amsterdam},
article_number = {December 2021},
doi = {http://dx.doi.org/10.1016/j.scr.2021.102574},
keywords = {human embryonic stem cells; Xeno- and feeder-free derivation; two sex-discordant sibling lines},
language = {eng},
issn = {1873-5061},
journal = {Stem Cell Research},
title = {Xeno- and feeder-free derivation of two sex-discordant sibling lines of human embryonic stem cells},
url = {https://www.sciencedirect.com/science/article/pii/S1873506121004219?via%3Dihub},
volume = {57},
year = {2021}
}
TY - JOUR
ID - 1799997
AU - Souralová, Tereza - Holubcová, Zuzana - Kyjovská, Drahomíra - Hampl, Aleš - Koutná, Irena
PY - 2021
TI - Xeno- and feeder-free derivation of two sex-discordant sibling lines of human embryonic stem cells
JF - Stem Cell Research
VL - 57
IS - December 2021
SP - 1-4
EP - 1-4
PB - Elsevier
SN - 18735061
KW - human embryonic stem cells
KW - Xeno- and feeder-free derivation
KW - two sex-discordant sibling lines
UR - https://www.sciencedirect.com/science/article/pii/S1873506121004219?via%3Dihub
N2 - Human embryonic stem cells (hESCs) represent a virtually unlimited source of cells suitable for a variety of biomedical applications. However, a diminishing allogeneic background and undefined culture conditions are essential for developing robust and replicable protocols for differentiation experiments, disease modeling, and drug testing. Therefore, here we report the generation of the two sex-discordant sibling hESC lines, MUNIe008-A and MUNIe009-A, using the mechanical biopsy of vitrified-thawed embryos under xeno- and feeder-free conditions. The presented approach is applicable for deriving high-quality clinical-grade hESC lines for cell replacement therapies.
ER -
SOURALOVÁ, Tereza, Zuzana HOLUBCOVÁ, Drahomíra KYJOVSKÁ, Aleš HAMPL a Irena KOUTNÁ. Xeno- and feeder-free derivation of two sex-discordant sibling lines of human embryonic stem cells. \textit{Stem Cell Research}. Amsterdam: Elsevier, 2021, roč.~57, December 2021, s.~1-4. ISSN~1873-5061. Dostupné z: https://dx.doi.org/10.1016/j.scr.2021.102574.
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