Determination of 8-hydroxy-2 '-deoxyguanosine in urine with

J 2021

Determination of 8-hydroxy-2 '-deoxyguanosine in urine with "linear" mode sparked graphite screen-printed electrodes

TRACHIOTI, Maria G., Jan HRBÁČ a Mamas I. PRODROMIDIS

Základní údaje

Originální název

Determination of 8-hydroxy-2 '-deoxyguanosine in urine with "linear" mode sparked graphite screen-printed electrodes

Autoři

TRACHIOTI, Maria G. (300 Řecko), Jan HRBÁČ (203 Česká republika, domácí) a Mamas I. PRODROMIDIS (300 Řecko, garant)

Vydání

Electrochimica Acta, Elsevier, 2021, 0013-4686

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10405 Electrochemistry

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 7.336

Kód RIV

RIV/00216224:14310/21:00122813

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.electacta.2021.139371

UT WoS

000711318400001

Klíčová slova anglicky

8−hydroxy−2ˊ−deoxyguanosineUrine biomarkersElectrode modification; Screen-printed electrode; Sparking discharge; DNA oxidative damage

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 1. 12. 2021 17:35, Mgr. Marie Šípková, DiS.

Anotace

V originále

The development of sensors for diagnostic purposes is of immense importance. Despite the number of proposed sensors showing attractive detection capabilities, the widespread use of these sensors is mainly hindered by selectivity issues and the elaborate tailoring of the sensing surface that eventually increases both the cost of the final sensor and the individual analysis cost. Herein, we report on the development of an advanced sensor for 8−hydroxy−2ˊ−deoxyguanosine (8−OHdG), an important biomarker for DNA oxidative damage. The sensor was developed on a low-cost graphite screen-printed electrode (SPE) by employing for the first time, an extremely fast SPE-to-graphite “linear” mode sparking process that enables the uniform modification of the whole electrode surface in less than 20 s. Graphite-sparked SPEs exhibited a linear relationship with the concentration of 8−OHdG over the range 2–50 nM, while the limit of detection (3σ) was 0.35 nM. The sensors showed a notable resistance to interference by 1000-fold excess of dopamine and ascorbic acid, and 100-fold excess of uric acid. A simple treatment of the samples, based on uricase, that eliminates the interference caused by uric acid under real-world conditions (>1000-fold excess) was optimized and proposed. The method was successfully applied to the determination of 8−OHdG in synthetic urine samples. Recovery was 95%.

Citovat

TRACHIOTI, Maria G., Jan HRBÁČ a Mamas I. PRODROMIDIS. Determination of 8-hydroxy-2 '-deoxyguanosine in urine with "linear" mode sparked graphite screen-printed electrodes. Electrochimica Acta. Elsevier, 2021, roč. 399, December, s. 1-9. ISSN 0013-4686. Dostupné z: https://dx.doi.org/10.1016/j.electacta.2021.139371.

@article{1802545,
   author = {Trachioti, Maria G. and Hrbáč, Jan and Prodromidis, Mamas I.},
   article_number = {December},
   doi = {http://dx.doi.org/10.1016/j.electacta.2021.139371},
   keywords = {8−hydroxy−2ˊ−deoxyguanosineUrine biomarkersElectrode modification; Screen-printed electrode; Sparking discharge; DNA oxidative damage},
   language = {eng},
   issn = {0013-4686},
   journal = {Electrochimica Acta},
   title = {Determination of 8-hydroxy-2 '-deoxyguanosine in urine with "linear" mode sparked graphite screen-printed electrodes},
   url = {https://www.sciencedirect.com/science/article/pii/S0013468621016613?via%3Dihub},
   volume = {399},
   year = {2021}
}

TY  - JOUR
ID  - 1802545
AU  - Trachioti, Maria G. - Hrbáč, Jan - Prodromidis, Mamas I.
PY  - 2021
TI  - Determination of 8-hydroxy-2 '-deoxyguanosine in urine with "linear" mode sparked graphite screen-printed electrodes
JF  - Electrochimica Acta
VL  - 399
IS  - December
SP  - 1-9
EP  - 1-9
PB  - Elsevier
SN  - 00134686
KW  - 8−hydroxy−2ˊ−deoxyguanosineUrine biomarkersElectrode modification
KW  - Screen-printed electrode
KW  - Sparking discharge
KW  - DNA oxidative damage
UR  - https://www.sciencedirect.com/science/article/pii/S0013468621016613?via%3Dihub
N2  - The development of sensors for diagnostic purposes is of immense importance. Despite the number of proposed sensors showing attractive detection capabilities, the widespread use of these sensors is mainly hindered by selectivity issues and the elaborate tailoring of the sensing surface that eventually increases both the cost of the final sensor and the individual analysis cost. Herein, we report on the development of an advanced sensor for 8−hydroxy−2ˊ−deoxyguanosine (8−OHdG), an important biomarker for DNA oxidative damage. The sensor was developed on a low-cost graphite screen-printed electrode (SPE) by employing for the first time, an extremely fast SPE-to-graphite “linear” mode sparking process that enables the uniform modification of the whole electrode surface in less than 20 s. Graphite-sparked SPEs exhibited a linear relationship with the concentration of 8−OHdG over the range 2–50 nM, while the limit of detection (3σ) was 0.35 nM. The sensors showed a notable resistance to interference by 1000-fold excess of dopamine and ascorbic acid, and 100-fold excess of uric acid. A simple treatment of the samples, based on uricase, that eliminates the interference caused by uric acid under real-world conditions (>1000-fold excess) was optimized and proposed. The method was successfully applied to the determination of 8−OHdG in synthetic urine samples. Recovery was 95%.
ER  -

TRACHIOTI, Maria G., Jan HRBÁČ a Mamas I. PRODROMIDIS. Determination of 8-hydroxy-2 '-deoxyguanosine in urine with ''linear'' mode sparked graphite screen-printed electrodes. \textit{Electrochimica Acta}. Elsevier, 2021, roč.~399, December, s.~1-9. ISSN~0013-4686. Dostupné z: https://dx.doi.org/10.1016/j.electacta.2021.139371.

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