Small RNA Sequencing Identifies PIWI-Interacting RNAs Deregulated in Glioblastoma-piR-9491 and ...

@article{1804764,
   author = {Bartos, M. and Siegl, František and Kopková, Alena and Radová, Lenka and Oppelt, Jan and Večeřa, Marek and Kazda, Tomáš and Jančálek, Radim and Hendrych, Michal and Hermanová, Markéta and Kasparova, P. and Pleskacova, Z. and Vybíhal, Václav and Fadrus, P. and Smrcka, M. and Lakomy, R. and Lipina, R. and Cesak, T. and Slabý, Ondřej and Šána, Jiří},
   article_location = {Lausanne},
   article_number = {AUG},
   doi = {http://dx.doi.org/10.3389/fonc.2021.707017},
   keywords = {glioblastoma; PIWI-interacting RNA; piRNA; diagnosis; prognosis},
   language = {eng},
   issn = {2234-943X},
   journal = {Frontiers in Oncology},
   title = {Small RNA Sequencing Identifies PIWI-Interacting RNAs Deregulated in Glioblastoma-piR-9491 and piR-12488 Reduce Tumor Cell Colonies In Vitro},
   url = {https://www.frontiersin.org/articles/10.3389/fonc.2021.707017/full},
   volume = {11},
   year = {2021}
}

TY  - JOUR
ID  - 1804764
AU  - Bartos, M. - Siegl, František - Kopková, Alena - Radová, Lenka - Oppelt, Jan - Večeřa, Marek - Kazda, Tomáš - Jančálek, Radim - Hendrych, Michal - Hermanová, Markéta - Kasparova, P. - Pleskacova, Z. - Vybíhal, Václav - Fadrus, P. - Smrcka, M. - Lakomy, R. - Lipina, R. - Cesak, T. - Slabý, Ondřej - Šána, Jiří
PY  - 2021
TI  - Small RNA Sequencing Identifies PIWI-Interacting RNAs Deregulated in Glioblastoma-piR-9491 and piR-12488 Reduce Tumor Cell Colonies In Vitro
JF  - Frontiers in Oncology
VL  - 11
IS  - AUG
SP  - 1-11
EP  - 1-11
PB  - Frontiers Media S.A.
SN  - 2234943X
KW  - glioblastoma
KW  - PIWI-interacting RNA
KW  - piRNA
KW  - diagnosis
KW  - prognosis
UR  - https://www.frontiersin.org/articles/10.3389/fonc.2021.707017/full
N2  - Glioblastoma (GBM) is the most frequently occurring primary malignant brain tumor of astrocytic origin. To change poor prognosis, it is necessary to deeply understand the molecular mechanisms of gliomagenesis and identify new potential biomarkers and therapeutic targets. PIWI-interacting RNAs (piRNAs) help in maintaining genome stability, and their deregulation has already been observed in many tumors. Recent studies suggest that these molecules could also play an important role in the glioma biology. To determine GBM-associated piRNAs, we performed small RNA sequencing analysis in the discovery set of 19 GBM and 11 non-tumor brain samples followed by TaqMan qRT-PCR analyses in the independent set of 77 GBM and 23 non-tumor patients. Obtained data were subsequently bioinformatically analyzed. Small RNA sequencing revealed 58 significantly deregulated piRNA molecules in GBM samples in comparison with non-tumor brain tissues. Deregulation of piR-1849, piR-9491, piR-12487, and piR-12488 was successfully confirmed in the independent groups of patients and controls (all p < 0.0001), and piR-9491 and piR-12488 reduced GBM cells' ability to form colonies in vitro. In addition, piR-23231 was significantly associated with the overall survival of the GBM patients treated with Stupp regimen (p = 0.007). Our results suggest that piRNAs could be a novel promising diagnostic and prognostic biomarker in GBM potentially playing important roles in gliomagenesis.
ER  - 

BARTOS, M., František SIEGL, Alena KOPKOVÁ, Lenka RADOVÁ, Jan OPPELT, Marek VEČEŘA, Tomáš KAZDA, Radim JANČÁLEK, Michal HENDRYCH, Markéta HERMANOVÁ, P. KASPAROVA, Z. PLESKACOVA, Václav VYBÍHAL, P. FADRUS, M. SMRCKA, R. LAKOMY, R. LIPINA, T. CESAK, Ondřej SLABÝ a Jiří ŠÁNA. Small RNA Sequencing Identifies PIWI-Interacting RNAs Deregulated in Glioblastoma-piR-9491 and piR-12488 Reduce Tumor Cell Colonies In Vitro. \textit{Frontiers in Oncology}. Lausanne: Frontiers Media S.A., 2021, roč.~11, AUG, s.~1-11. ISSN~2234-943X. Dostupné z: https://dx.doi.org/10.3389/fonc.2021.707017.