Role of miR-653 and miR-29c in downregulation of CYP1A2
expression in hepatocellular carcinoma

J 2022

Role of miR-653 and miR-29c in downregulation of CYP1A2 expression in hepatocellular carcinoma

KRKOŠKA, Martin; Jana NEKVINDOVA; Kateřina NEVĚDĚLOVÁ; Veronika ZUBANOVA; Lenka RADOVÁ et al.

Základní údaje

Originální název

Role of miR-653 and miR-29c in downregulation of CYP1A2 expression in hepatocellular carcinoma

Autoři

Vydání

Pharmacological Reports, KRAKOW, POLISH ACAD SCIENCES INST PHARMACOLOGY, 2022, 1734-1140

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30104 Pharmacology and pharmacy

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.400

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/22:00125067

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

CYP1A2; Hepatocellular carcinoma; Biotransformation; microRNA; AHR

Štítky

14110212, 14110223, 14110513, 14110811, 14314010, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 3. 2022 08:54, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Background Hepatocellular carcinoma (HCC) is a major contributor to the worldwide cancer burden. Recent studies on HCC have demonstrated dramatic alterations in expression of several cytochrome P450 (CYP) family members that play a crucial role in biotransformation of many drugs and other xenobiotics; however, the mechanisms responsible for their deregulation remain unclear. Methods We investigated a potential involvement of miRNAs in downregulation of expression of CYPs observed in HCC tumors. We compared miRNA expression profiles (TaqMan Array Human MicroRNA v3.0 TLDA qPCR) between HCC human patient tumors with strong (CYP-) and weak/no (CYP+) downregulation of drug-metabolizing CYPs. The role of significantly deregulated miRNAs in modulation of expression of the CYPs and associated xenobiotic receptors was then investigated in human liver HepaRG cells transfected with relevant miRNA mimics or inhibitors. Results We identified five differentially expressed miRNAs in CYP- versus CYP+ tumors, namely miR-29c, miR-125b1, miR-505, miR-653 and miR-675. The two most-upregulated miRNAs found in CYP- tumor samples, miR-29c and miR-653, were found to act as efficient suppressors of CYP1A2 or AHR expression. Conclusions Our results revealed a novel role of miR-653 and miR-29c in regulation of expresion of CYPs involved in crucial biotransformation processes in liver, which are often deregulated during liver cancer progression. [GRAPHICS] .

Přiložené soubory

Role_of_miR-653_and_miR-29c_in_downregulation_of_CYP1A2_expression_in_hepatocellular_carcinoma.pdf

Požádat o autorskou verzi souboru

Citovat

KRKOŠKA, Martin; Jana NEKVINDOVA; Kateřina NEVĚDĚLOVÁ; Veronika ZUBANOVA; Lenka RADOVÁ; Jan VONDRACEK; Jarmila HERUDKOVA; Ondřej SLABÝ; Igor KISS; Lucia BOHOVICOVÁ; Pavel FABIAN; Zuzana TYLICHOVA; Zdeněk KALA; Petr KYSELA; Lenka OSTŘÍŽKOVÁ; Vladimir PALICKA a Alena HYRŠLOVÁ VACULOVÁ. Role of miR-653 and miR-29c in downregulation of CYP1A2 expression in hepatocellular carcinoma. Pharmacological Reports. KRAKOW: POLISH ACAD SCIENCES INST PHARMACOLOGY, 2022, roč. 74, č. 1, s. 148-158. ISSN 1734-1140. Dostupné z: https://doi.org/10.1007/s43440-021-00338-9.

@article{1805239,
   author = {Krkoška, Martin and Nekvindova, Jana and Nevědělová, Kateřina and Zubanova, Veronika and Radová, Lenka and Vondracek, Jan and Herudkova, Jarmila and Slabý, Ondřej and Kiss, Igor and Bohovicová, Lucia and Fabian, Pavel and Tylichova, Zuzana and Kala, Zdeněk and Kysela, Petr and Ostřížková, Lenka and Palicka, Vladimir and Hyršlová Vaculová, Alena},
   article_location = {KRAKOW},
   article_number = {1},
   doi = {https://doi.org/10.1007/s43440-021-00338-9},
   keywords = {CYP1A2; Hepatocellular carcinoma; Biotransformation; microRNA; AHR},
   language = {eng},
   issn = {1734-1140},
   journal = {Pharmacological Reports},
   title = {Role of miR-653 and miR-29c in downregulation of CYP1A2 expression in hepatocellular carcinoma},
   url = {https://link.springer.com/article/10.1007%2Fs43440-021-00338-9},
   volume = {74},
   year = {2022}
}

TY  - JOUR
ID  - 1805239
AU  - Krkoška, Martin - Nekvindova, Jana - Nevědělová, Kateřina - Zubanova, Veronika - Radová, Lenka - Vondracek, Jan - Herudkova, Jarmila - Slabý, Ondřej - Kiss, Igor - Bohovicová, Lucia - Fabian, Pavel - Tylichova, Zuzana - Kala, Zdeněk - Kysela, Petr - Ostřížková, Lenka - Palicka, Vladimir - Hyršlová Vaculová, Alena
PY  - 2022
TI  - Role of miR-653 and miR-29c in downregulation of CYP1A2 expression in hepatocellular carcinoma
JF  - Pharmacological Reports
VL  - 74
IS  - 1
SP  - 148-158
EP  - 148-158
PB  - POLISH ACAD SCIENCES INST PHARMACOLOGY
SN  - 17341140
KW  - CYP1A2
KW  - Hepatocellular carcinoma
KW  - Biotransformation
KW  - microRNA
KW  - AHR
UR  - https://link.springer.com/article/10.1007%2Fs43440-021-00338-9
N2  - Background Hepatocellular carcinoma (HCC) is a major contributor to the worldwide cancer burden. Recent studies on HCC have demonstrated dramatic alterations in expression of several cytochrome P450 (CYP) family members that play a crucial role in biotransformation of many drugs and other xenobiotics; however, the mechanisms responsible for their deregulation remain unclear. Methods We investigated a potential involvement of miRNAs in downregulation of expression of CYPs observed in HCC tumors. We compared miRNA expression profiles (TaqMan Array Human MicroRNA v3.0 TLDA qPCR) between HCC human patient tumors with strong (CYP-) and weak/no (CYP+) downregulation of drug-metabolizing CYPs. The role of significantly deregulated miRNAs in modulation of expression of the CYPs and associated xenobiotic receptors was then investigated in human liver HepaRG cells transfected with relevant miRNA mimics or inhibitors. Results We identified five differentially expressed miRNAs in CYP- versus CYP+ tumors, namely miR-29c, miR-125b1, miR-505, miR-653 and miR-675. The two most-upregulated miRNAs found in CYP- tumor samples, miR-29c and miR-653, were found to act as efficient suppressors of CYP1A2 or AHR expression. Conclusions Our results revealed a novel role of miR-653 and miR-29c in regulation of expresion of CYPs involved in crucial biotransformation processes in liver, which are often deregulated during liver cancer progression. [GRAPHICS] .
ER  -

KRKOŠKA, Martin; Jana NEKVINDOVA; Kateřina NEVĚDĚLOVÁ; Veronika ZUBANOVA; Lenka RADOVÁ; Jan VONDRACEK; Jarmila HERUDKOVA; Ondřej SLABÝ; Igor KISS; Lucia BOHOVICOVÁ; Pavel FABIAN; Zuzana TYLICHOVA; Zdeněk KALA; Petr KYSELA; Lenka OSTŘÍŽKOVÁ; Vladimir PALICKA a Alena HYRŠLOVÁ VACULOVÁ. Role of miR-653 and miR-29c in downregulation of CYP1A2 expression in hepatocellular carcinoma. \textit{Pharmacological Reports}. KRAKOW: POLISH ACAD SCIENCES INST PHARMACOLOGY, 2022, roč.~74, č.~1, s.~148-158. ISSN~1734-1140. Dostupné z: https://doi.org/10.1007/s43440-021-00338-9.

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