A simple electroanalysis of polyG RNA in mixtures with
3',5'-cyclic guanosine monophosphate achieved by selective
desorption of the monomers from the electrode surface

J 2021

A simple electroanalysis of polyG RNA in mixtures with 3',5'-cyclic guanosine monophosphate achieved by selective desorption of the monomers from the electrode surface

HESKO, Ondrej, Miroslav FOJTA a Jan ŠPAČEK

Základní údaje

Originální název

A simple electroanalysis of polyG RNA in mixtures with 3',5'-cyclic guanosine monophosphate achieved by selective desorption of the monomers from the electrode surface

Autoři

HESKO, Ondrej (703 Slovensko, domácí), Miroslav FOJTA a Jan ŠPAČEK (garant)

Vydání

Journal of Electroanalytical Chemistry, Lausanne, Elsevier, 2021, 1572-6657

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10405 Electrochemistry

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.598

Kód RIV

RIV/00216224:14310/21:00123011

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.jelechem.2021.115773

UT WoS

000714137900001

Klíčová slova anglicky

Electrochemistry; Desorption; Pyrolytic graphite electrode; Oligonucleotides; RNA; 3'; 5'-cGMP

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 26. 11. 2021 12:26, Mgr. Marie Šípková, DiS.

Anotace

V originále

Previously it has been shown that cyclic nucleoside monophosphates can spontaneously polymerize to form RNA oligonucleotides under conditions simulating prebiotic conditions on Archean Earth. The most efficient polymerization was documented with 3',5'-cyclic guanosine monophosphate (cGMP). In this work a method for fast detection of short polyG RNAs present in a large overabundance of cGMP, modeling conditions in the non-enzymatic nucleotide polymerization mixtures, is presented. The method is based on electrochemical measurements of guanine (G) oxidation signals yielded by RNA oligomers adsorbed onto the surface of a pyrolytic graphite electrode (PGE). To avoid false positive results arising from the G oxidation signals due to coadsorbed cGMP, a method for selective removal of the monomers from the electrode surface has been devised. In the first step, both cGMP and RNAs are co-adsorbed onto the PGE surface. In the second step, the cGMP is selectively desorbed using treatments in solutions of different tested surfactants (SDS, Tween 20 or Triton X100), or by washing in deionized water at elevated temperature. We show that this new approach is suitable for selective analysis of products of polymerization reactions from mixtures of their building blocks.

Citovat

HESKO, Ondrej, Miroslav FOJTA a Jan ŠPAČEK. A simple electroanalysis of polyG RNA in mixtures with 3',5'-cyclic guanosine monophosphate achieved by selective desorption of the monomers from the electrode surface. Journal of Electroanalytical Chemistry. Lausanne: Elsevier, 2021, roč. 901, November, s. 115773-115778. ISSN 1572-6657. Dostupné z: https://dx.doi.org/10.1016/j.jelechem.2021.115773.

@article{1805241,
   author = {Hesko, Ondrej and Fojta, Miroslav and Špaček, Jan},
   article_location = {Lausanne},
   article_number = {November},
   doi = {http://dx.doi.org/10.1016/j.jelechem.2021.115773},
   keywords = {Electrochemistry; Desorption; Pyrolytic graphite electrode; Oligonucleotides; RNA; 3'; 5'-cGMP},
   language = {eng},
   issn = {1572-6657},
   journal = {Journal of Electroanalytical Chemistry},
   title = {A simple electroanalysis of polyG RNA in mixtures with 3',5'-cyclic guanosine monophosphate achieved by selective desorption of the monomers from the electrode surface},
   url = {https://www.sciencedirect.com/science/article/pii/S1572665721007992},
   volume = {901},
   year = {2021}
}

TY  - JOUR
ID  - 1805241
AU  - Hesko, Ondrej - Fojta, Miroslav - Špaček, Jan
PY  - 2021
TI  - A simple electroanalysis of polyG RNA in mixtures with 3',5'-cyclic guanosine monophosphate achieved by selective desorption of the monomers from the electrode surface
JF  - Journal of Electroanalytical Chemistry
VL  - 901
IS  - November
SP  - 115773
EP  - 115773
PB  - Elsevier
SN  - 15726657
KW  - Electrochemistry
KW  - Desorption
KW  - Pyrolytic graphite electrode
KW  - Oligonucleotides
KW  - RNA
KW  - 3'
KW  - 5'-cGMP
UR  - https://www.sciencedirect.com/science/article/pii/S1572665721007992
N2  - Previously it has been shown that cyclic nucleoside monophosphates can spontaneously polymerize to form RNA oligonucleotides under conditions simulating prebiotic conditions on Archean Earth. The most efficient polymerization was documented with 3',5'-cyclic guanosine monophosphate (cGMP). In this work a method for fast detection of short polyG RNAs present in a large overabundance of cGMP, modeling conditions in the non-enzymatic nucleotide polymerization mixtures, is presented. The method is based on electrochemical measurements of guanine (G) oxidation signals yielded by RNA oligomers adsorbed onto the surface of a pyrolytic graphite electrode (PGE). To avoid false positive results arising from the G oxidation signals due to coadsorbed cGMP, a method for selective removal of the monomers from the electrode surface has been devised. In the first step, both cGMP and RNAs are co-adsorbed onto the PGE surface. In the second step, the cGMP is selectively desorbed using treatments in solutions of different tested surfactants (SDS, Tween 20 or Triton X100), or by washing in deionized water at elevated temperature. We show that this new approach is suitable for selective analysis of products of polymerization reactions from mixtures of their building blocks.
ER  -

HESKO, Ondrej, Miroslav FOJTA a Jan ŠPAČEK. A simple electroanalysis of polyG RNA in mixtures with 3',5'-cyclic guanosine monophosphate achieved by selective desorption of the monomers from the electrode surface. \textit{Journal of Electroanalytical Chemistry}. Lausanne: Elsevier, 2021, roč.~901, November, s.~115773-115778. ISSN~1572-6657. Dostupné z: https://dx.doi.org/10.1016/j.jelechem.2021.115773.

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