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@article{1806823,
author = {Ruiz, N. and Jarošová, Petra and Táborský, Petr and Gargallo, Raimundo},
article_location = {AMSTERDAM},
article_number = {FEB},
doi = {http://dx.doi.org/10.1016/j.bpc.2021.106715},
keywords = {G-quadruplex; i-motif; Hybrid structures; Palmatine; Binding},
language = {eng},
issn = {0301-4622},
journal = {Biophysical Chemistry},
title = {Study of the interaction of the palmatine alkaloid with hybrid G-quadruplex/duplex and i-motif/duplex DNA structures},
url = {https://linkinghub.elsevier.com/retrieve/pii/S0301462221001988},
volume = {281},
year = {2022}
}
TY - JOUR
ID - 1806823
AU - Ruiz, N. - Jarošová, Petra - Táborský, Petr - Gargallo, Raimundo
PY - 2022
TI - Study of the interaction of the palmatine alkaloid with hybrid G-quadruplex/duplex and i-motif/duplex DNA structures
JF - Biophysical Chemistry
VL - 281
IS - FEB
SP - 1-8
EP - 1-8
PB - Elsevier
SN - 03014622
KW - G-quadruplex
KW - i-motif
KW - Hybrid structures
KW - Palmatine
KW - Binding
UR - https://linkinghub.elsevier.com/retrieve/pii/S0301462221001988
N2 - There is an increasing interest in the study of guanine or cytosine-rich sequences that may fold into G-quadruplex (G4) or i-motif (iM) structures showing a short hairpin (or stem-loop) stabilized by Watson-Crick base pairs. These hybrid spatial arrangements may be target of ligands that have been shown to interact strongly with BDNA. In this work, the interaction of the palmatine alkaloid with several sequences forming different G4s, iMs, and hybrid structures has been studied by means of spectroscopic and separation techniques, as well as multivariate data analysis methods. At the experimental conditions used in this work, the results have shown that this ligand strongly stabilizes parallel G4 structures, whereas a weaker interaction was observed with the antiparallel G4 adopted by the thrombin-binding aptamer or iMs. The presence of hairpins within the loops scarcely affects the affinity of this ligand for the hybrid G4/duplex or iM/duplex structures. Fluorescence measurements have provided evidence of a certain interaction with iMs at pH 5.1, despite the absence of thermal stabilization effects.
ER -
RUIZ, N., Petra JAROŠOVÁ, Petr TÁBORSKÝ a Raimundo GARGALLO. Study of the interaction of the palmatine alkaloid with hybrid G-quadruplex/duplex and i-motif/duplex DNA structures. \textit{Biophysical Chemistry}. AMSTERDAM: Elsevier, 2022, roč.~281, FEB, s.~1-8. ISSN~0301-4622. Dostupné z: https://dx.doi.org/10.1016/j.bpc.2021.106715.
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