Cardinium inhibits Wolbachia in its mite host, Tyrophagus
putrescentiae, and affects host fitness

J 2021

Cardinium inhibits Wolbachia in its mite host, Tyrophagus putrescentiae, and affects host fitness

HUBERT, Jan, Marta NESVORNA, Stanislav PEKÁR, Stefan J. GREEN, Pavel B. KLIMOV et. al.

Základní údaje

Originální název

Cardinium inhibits Wolbachia in its mite host, Tyrophagus putrescentiae, and affects host fitness

Autoři

HUBERT, Jan (garant), Marta NESVORNA, Stanislav PEKÁR (703 Slovensko, domácí), Stefan J. GREEN a Pavel B. KLIMOV

Vydání

FEMS Microbiology Ecology, Oxford, England, Oxford University Press, 2021, 0168-6496

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.519

Kód RIV

RIV/00216224:14310/21:00123195

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1093/femsec/fiab123

UT WoS

000733861000001

Klíčová slova anglicky

Bartonella; Cardinium; Fitness; Intracellular symbionts; Mite; Reproduction; Wolbachia

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 21. 1. 2022 08:35, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Interactions among endosymbiotic bacteria inside their eukaryotic hosts are poorly understood, particularly in mites. The mite Tyrophagus putrescentiae is a common, medically important generalist species that has many intracellular and gut bacterial symbionts. In the experiments, we examined bacterial abundances and composition in mite populations obtained by controlled mixing of stock mite populations that differed in the presence/absence of the major intracellular bacteria Wolbachia and Cardinium. Changes in microbial communities were characterized using 16S ribosomal RNA high-throughput sequencing (pooled mite individuals) and quantitative PCR for key microbial taxa (individual mites). Mite fitness was estimated as a parameter of population growth. We detected that in mixed mite populations, Cardinium and Wolbachia can co-occur in the same mite individual. The presence of Cardinium was negatively correlated with the presence of Wolbachia and Bartonella, while the Bartonella and Wolbachia were positively correlated in individual level samples. Since mixed populations had lower abundances of Wolbachia, while the abundance of Cardinium did not change, we suggest that the presence of Cardinium inhibits the growth of Wolbachia. The mixed mite populations had lower population growth than parental populations. The possible effect of symbionts on the fitness of mixed population is discussed.

Citovat

HUBERT, Jan, Marta NESVORNA, Stanislav PEKÁR, Stefan J. GREEN a Pavel B. KLIMOV. Cardinium inhibits Wolbachia in its mite host, Tyrophagus putrescentiae, and affects host fitness. FEMS Microbiology Ecology. Oxford, England: Oxford University Press, 2021, roč. 97, č. 10, s. "fiab123", 10 s. ISSN 0168-6496. Dostupné z: https://dx.doi.org/10.1093/femsec/fiab123.

@article{1808489,
   author = {Hubert, Jan and Nesvorna, Marta and Pekár, Stanislav and Green, Stefan J. and Klimov, Pavel B.},
   article_location = {Oxford, England},
   article_number = {10},
   doi = {http://dx.doi.org/10.1093/femsec/fiab123},
   keywords = {Bartonella; Cardinium; Fitness; Intracellular symbionts; Mite; Reproduction; Wolbachia},
   language = {eng},
   issn = {0168-6496},
   journal = {FEMS Microbiology Ecology},
   title = {Cardinium inhibits Wolbachia in its mite host, Tyrophagus putrescentiae, and affects host fitness},
   url = {https://doi.org/10.1093/femsec/fiab123},
   volume = {97},
   year = {2021}
}

TY  - JOUR
ID  - 1808489
AU  - Hubert, Jan - Nesvorna, Marta - Pekár, Stanislav - Green, Stefan J. - Klimov, Pavel B.
PY  - 2021
TI  - Cardinium inhibits Wolbachia in its mite host, Tyrophagus putrescentiae, and affects host fitness
JF  - FEMS Microbiology Ecology
VL  - 97
IS  - 10
SP  - "fiab123"
EP  - "fiab123"
PB  - Oxford University Press
SN  - 01686496
KW  - Bartonella
KW  - Cardinium
KW  - Fitness
KW  - Intracellular symbionts
KW  - Mite
KW  - Reproduction
KW  - Wolbachia
UR  - https://doi.org/10.1093/femsec/fiab123
N2  - Interactions among endosymbiotic bacteria inside their eukaryotic hosts are poorly understood, particularly in mites. The mite Tyrophagus putrescentiae is a common, medically important generalist species that has many intracellular and gut bacterial symbionts. In the experiments, we examined bacterial abundances and composition in mite populations obtained by controlled mixing of stock mite populations that differed in the presence/absence of the major intracellular bacteria Wolbachia and Cardinium. Changes in microbial communities were characterized using 16S ribosomal RNA high-throughput sequencing (pooled mite individuals) and quantitative PCR for key microbial taxa (individual mites). Mite fitness was estimated as a parameter of population growth. We detected that in mixed mite populations, Cardinium and Wolbachia can co-occur in the same mite individual. The presence of Cardinium was negatively correlated with the presence of Wolbachia and Bartonella, while the Bartonella and Wolbachia were positively correlated in individual level samples. Since mixed populations had lower abundances of Wolbachia, while the abundance of Cardinium did not change, we suggest that the presence of Cardinium inhibits the growth of Wolbachia. The mixed mite populations had lower population growth than parental populations. The possible effect of symbionts on the fitness of mixed population is discussed.
ER  -

HUBERT, Jan, Marta NESVORNA, Stanislav PEKÁR, Stefan J. GREEN a Pavel B. KLIMOV. Cardinium inhibits Wolbachia in its mite host, Tyrophagus putrescentiae, and affects host fitness. \textit{FEMS Microbiology Ecology}. Oxford, England: Oxford University Press, 2021, roč.~97, č.~10, s.~''fiab123'', 10 s. ISSN~0168-6496. Dostupné z: https://dx.doi.org/10.1093/femsec/fiab123.

Podrobný výpis o publikaci