Pyocin-mediated antagonistic interactions in Pseudomonas spp.
isolated in James Ross Island, Antarctica

J 2022

Pyocin-mediated antagonistic interactions in Pseudomonas spp. isolated in James Ross Island, Antarctica

SNOPKOVÁ, Kateřina, Kristýna DUFKOVÁ, Ivo CHAMRÁD, René LENOBEL, Darina ČEJKOVÁ et. al.

Základní údaje

Originální název

Pyocin-mediated antagonistic interactions in Pseudomonas spp. isolated in James Ross Island, Antarctica

Autoři

SNOPKOVÁ, Kateřina (203 Česká republika, domácí), Kristýna DUFKOVÁ (203 Česká republika, domácí), Ivo CHAMRÁD (203 Česká republika), René LENOBEL (203 Česká republika), Darina ČEJKOVÁ (203 Česká republika), Marcel KOSINA (203 Česká republika, domácí), Matěj HRALA (203 Česká republika, domácí), Veronika HOLÁ (203 Česká republika, domácí), Ivo SEDLÁČEK (203 Česká republika, domácí) a David ŠMAJS (203 Česká republika, garant, domácí)

Vydání

ENVIRONMENTAL MICROBIOLOGY, HOBOKEN, WILEY, 2022, 1462-2912

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.100

Kód RIV

RIV/00216224:14110/22:00125088

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1111/1462-2920.15809

UT WoS

000714374200001

Klíčová slova anglicky

James Ross Island; Antarctica; Psychrotrophic Bacteria; P. aeruginosa; Microorganisms; Sequences; Plant; Phage; Pseudomonas; sp. nov.

Štítky

14110113, 14110513, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 27. 3. 2023 15:35, Mgr. Marie Šípková, DiS.

Anotace

V originále

Interactions within bacterial communities are frequently mediated by the production of antimicrobial agents. Despite the increasing interest in research of new antimicrobials, studies describing antagonistic interactions among cold-adapted microorganisms are still rare. Our study assessed the antimicrobial interactions of 36 Antarctic Pseudomonas spp. and described the genetic background of these interactions in selected strains. The overall bacteriocinogeny was greater compared to mesophilic Pseudomonas non-aeruginosa species. R-type tailocins were detected on transmission electron micrographs in 16 strains (44.4%); phylogenetic analysis of the corresponding gene clusters revealed that the P. prosekii CCM 8878 tailocin was related to the Rp3 group, whereas the tailocin in Pseudomonas sp. CCM 8880 to the Rp4 group. Soluble antimicrobials were produced by eight strains (22.-2%); gene mining found pyocin L homologues in the genomes of P. prosekii CCM 8881 and CCM 8879 and pyocin S9-like homologues in P. prosekii CCM 8881 and Pseudomonas sp. CCM 8880. Analysis of secretomes confirmed the production of all S- and L-type pyocin genes. Our results suggest that bacteriocin-based inhibition plays an important role in interactions among Antarctic soil bacteria, and these native, cold-adapted microorganisms could be a promising source of new antimicrobials.

Návaznosti

LM2015078, projekt VaV

Název: Česká polární výzkumná infrastruktura (Akronym: CzechPolar2)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Polar Research Infrastructure

LM2015091, projekt VaV

Název: Národní centrum lékařské genomiky (Akronym: NCLG)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Národní centrum lékařské genomiky

MUNI/A/1325/2020, interní kód MU

Název: Biomedicínské vědy

Investor: Masarykova univerzita, Biomedicínské vědy

MUNI/A/1486/2020, interní kód MU

Název: Nové možnosti detekce a terapie nozokomiálních patogenů (Akronym: NMDTNP)

Investor: Masarykova univerzita, Nové možnosti detekce a terapie nozokomiálních patogenů

Citovat

SNOPKOVÁ, Kateřina, Kristýna DUFKOVÁ, Ivo CHAMRÁD, René LENOBEL, Darina ČEJKOVÁ, Marcel KOSINA, Matěj HRALA, Veronika HOLÁ, Ivo SEDLÁČEK a David ŠMAJS. Pyocin-mediated antagonistic interactions in Pseudomonas spp. isolated in James Ross Island, Antarctica. ENVIRONMENTAL MICROBIOLOGY. HOBOKEN: WILEY, 2022, roč. 24, č. 3, s. 1294-1307. ISSN 1462-2912. Dostupné z: https://dx.doi.org/10.1111/1462-2920.15809.

@article{1808897,
   author = {Snopková, Kateřina and Dufková, Kristýna and Chamrád, Ivo and Lenobel, René and Čejková, Darina and Kosina, Marcel and Hrala, Matěj and Holá, Veronika and Sedláček, Ivo and Šmajs, David},
   article_location = {HOBOKEN},
   article_number = {3},
   doi = {http://dx.doi.org/10.1111/1462-2920.15809},
   keywords = {James Ross Island; Antarctica; Psychrotrophic Bacteria; P. aeruginosa; Microorganisms; Sequences; Plant; Phage; Pseudomonas; sp. nov.},
   language = {eng},
   issn = {1462-2912},
   journal = {ENVIRONMENTAL MICROBIOLOGY},
   title = {Pyocin-mediated antagonistic interactions in Pseudomonas spp. isolated in James Ross Island, Antarctica},
   url = {https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/1462-2920.15809},
   volume = {24},
   year = {2022}
}

TY  - JOUR
ID  - 1808897
AU  - Snopková, Kateřina - Dufková, Kristýna - Chamrád, Ivo - Lenobel, René - Čejková, Darina - Kosina, Marcel - Hrala, Matěj - Holá, Veronika - Sedláček, Ivo - Šmajs, David
PY  - 2022
TI  - Pyocin-mediated antagonistic interactions in Pseudomonas spp. isolated in James Ross Island, Antarctica
JF  - ENVIRONMENTAL MICROBIOLOGY
VL  - 24
IS  - 3
SP  - 1294-1307
EP  - 1294-1307
PB  - WILEY
SN  - 14622912
KW  - James Ross Island
KW  - Antarctica
KW  - Psychrotrophic Bacteria
KW  - P. aeruginosa
KW  - Microorganisms
KW  - Sequences
KW  - Plant
KW  - Phage
KW  - Pseudomonas
KW  - sp. nov.
UR  - https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/1462-2920.15809
N2  - Interactions within bacterial communities are frequently mediated by the production of antimicrobial agents. Despite the increasing interest in research of new antimicrobials, studies describing antagonistic interactions among cold-adapted microorganisms are still rare. Our study assessed the antimicrobial interactions of 36 Antarctic Pseudomonas spp. and described the genetic background of these interactions in selected strains. The overall bacteriocinogeny was greater compared to mesophilic Pseudomonas non-aeruginosa species. R-type tailocins were detected on transmission electron micrographs in 16 strains (44.4%); phylogenetic analysis of the corresponding gene clusters revealed that the P. prosekii CCM 8878 tailocin was related to the Rp3 group, whereas the tailocin in Pseudomonas sp. CCM 8880 to the Rp4 group. Soluble antimicrobials were produced by eight strains (22.-2%); gene mining found pyocin L homologues in the genomes of P. prosekii CCM 8881 and CCM 8879 and pyocin S9-like homologues in P. prosekii CCM 8881 and Pseudomonas sp. CCM 8880. Analysis of secretomes confirmed the production of all S- and L-type pyocin genes. Our results suggest that bacteriocin-based inhibition plays an important role in interactions among Antarctic soil bacteria, and these native, cold-adapted microorganisms could be a promising source of new antimicrobials.
ER  -

SNOPKOVÁ, Kateřina, Kristýna DUFKOVÁ, Ivo CHAMRÁD, René LENOBEL, Darina ČEJKOVÁ, Marcel KOSINA, Matěj HRALA, Veronika HOLÁ, Ivo SEDLÁČEK a David ŠMAJS. Pyocin-mediated antagonistic interactions in Pseudomonas spp. isolated in James Ross Island, Antarctica. \textit{ENVIRONMENTAL MICROBIOLOGY}. HOBOKEN: WILEY, 2022, roč.~24, č.~3, s.~1294-1307. ISSN~1462-2912. Dostupné z: https://dx.doi.org/10.1111/1462-2920.15809.

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