RGDS-Modified Superporous Poly(2-Hydroxyethyl
Methacrylate)-Based Scaffolds as 3D In Vitro Leukemia Model

J 2021

RGDS-Modified Superporous Poly(2-Hydroxyethyl Methacrylate)-Based Scaffolds as 3D In Vitro Leukemia Model

SVOZILOVÁ, Hana, Z. PLICHTA, V. PROKS, Radana STUDENÁ, Jiří BALOUN et. al.

Základní údaje

Originální název

RGDS-Modified Superporous Poly(2-Hydroxyethyl Methacrylate)-Based Scaffolds as 3D In Vitro Leukemia Model

Autoři

SVOZILOVÁ, Hana (203 Česká republika, domácí), Z. PLICHTA, V. PROKS, Radana STUDENÁ (203 Česká republika, domácí), Jiří BALOUN (203 Česká republika, domácí), Michael DOUBEK (203 Česká republika, domácí), Šárka POSPÍŠILOVÁ (203 Česká republika, garant, domácí) a D. HORAK

Vydání

International Journal of Molecular Sciences, Basel, Multidisciplinary Digital Publishing Institute, 2021, 1422-0067

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.208

Kód RIV

RIV/00216224:14740/21:00123378

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.3390/ijms22052376

UT WoS

000628297600001

Klíčová slova anglicky

poly(2-hydroxyethyl methacrylate); 3D scaffold; RGDS; chronic lymphocytic leukemia; B cell survival

Štítky

14110212, CF CELLIM, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 8. 10. 2024 10:15, Ing. Martina Blahová

Anotace

V originále

Superporous poly(2-hydroxyethyl methacrylate-co-2-aminoethyl methacrylate) (P(HEMA-AEMA)) hydrogel scaffolds are designed for in vitro 3D culturing of leukemic B cells. Hydrogel porosity, which influences cell functions and growth, is introduced by adding ammonium oxalate needle-like crystals in the polymerization mixture. To improve cell vitality, cell-adhesive Arg-Gly-Asp-Ser (RGDS) peptide is immobilized on the N-(gamma-maleimidobutyryloxy)succinimide-activated P(HEMA-AEMA) hydrogels via reaction of SH with maleimide groups. This modification is especially suitable for the survival of primary chronic lymphocytic leukemia cells (B-CLLs) in 3D cell culture. No other tested stimuli (interleukin-4, CD40 ligand, or shaking) can further improve B-CLL survival or metabolic activity. Both unmodified and RGDS-modified P(HEMA-AEMA) scaffolds serve as a long-term (70 days) 3D culture platforms for HS-5 and M2-10B4 bone marrow stromal cell lines and MEC-1 and HG-3 B-CLL cell lines, although the adherent cells retain their physiological morphologies, preferably on RGDS-modified hydrogels. Moreover, the porosity of hydrogels allows direct cell lysis, followed by efficient DNA isolation from the 3D-cultured cells. P(HEMA-AEMA)-RGDS thus serves as a suitable 3D in vitro leukemia model that enables molecular and metabolic assays and allows imaging of cell morphology, interactions, and migration by confocal microscopy. Such applications can prospectively assist in testing of drugs to treat this frequently recurring or refractory cancer.

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

MUNI/A/1595/2020, interní kód MU

Název: Nové přístupy ve výzkumu, diagnostice a terapii hematologických malignit VIII (Akronym: VýDiTeHeMa VIII)

Investor: Masarykova univerzita, Nové přístupy ve výzkumu, diagnostice a terapii hematologických malignit VIII

90129, velká výzkumná infrastruktura

Název: Czech-BioImaging II

Citovat

SVOZILOVÁ, Hana, Z. PLICHTA, V. PROKS, Radana STUDENÁ, Jiří BALOUN, Michael DOUBEK, Šárka POSPÍŠILOVÁ a D. HORAK. RGDS-Modified Superporous Poly(2-Hydroxyethyl Methacrylate)-Based Scaffolds as 3D In Vitro Leukemia Model. International Journal of Molecular Sciences. Basel: Multidisciplinary Digital Publishing Institute, 2021, roč. 22, č. 5, s. 2376-2392. ISSN 1422-0067. Dostupné z: https://dx.doi.org/10.3390/ijms22052376.

@article{1813387,
   author = {Svozilová, Hana and Plichta, Z. and Proks, V. and Studená, Radana and Baloun, Jiří and Doubek, Michael and Pospíšilová, Šárka and Horak, D.},
   article_location = {Basel},
   article_number = {5},
   doi = {http://dx.doi.org/10.3390/ijms22052376},
   keywords = {poly(2-hydroxyethyl methacrylate); 3D scaffold; RGDS; chronic lymphocytic leukemia; B cell survival},
   language = {eng},
   issn = {1422-0067},
   journal = {International Journal of Molecular Sciences},
   title = {RGDS-Modified Superporous Poly(2-Hydroxyethyl Methacrylate)-Based Scaffolds as 3D In Vitro Leukemia Model},
   url = {https://www.mdpi.com/1422-0067/22/5/2376},
   volume = {22},
   year = {2021}
}

TY  - JOUR
ID  - 1813387
AU  - Svozilová, Hana - Plichta, Z. - Proks, V. - Studená, Radana - Baloun, Jiří - Doubek, Michael - Pospíšilová, Šárka - Horak, D.
PY  - 2021
TI  - RGDS-Modified Superporous Poly(2-Hydroxyethyl Methacrylate)-Based Scaffolds as 3D In Vitro Leukemia Model
JF  - International Journal of Molecular Sciences
VL  - 22
IS  - 5
SP  - 2376
EP  - 2376
PB  - Multidisciplinary Digital Publishing Institute
SN  - 14220067
KW  - poly(2-hydroxyethyl methacrylate)
KW  - 3D scaffold
KW  - RGDS
KW  - chronic lymphocytic leukemia
KW  - B cell survival
UR  - https://www.mdpi.com/1422-0067/22/5/2376
N2  - Superporous poly(2-hydroxyethyl methacrylate-co-2-aminoethyl methacrylate) (P(HEMA-AEMA)) hydrogel scaffolds are designed for in vitro 3D culturing of leukemic B cells. Hydrogel porosity, which influences cell functions and growth, is introduced by adding ammonium oxalate needle-like crystals in the polymerization mixture. To improve cell vitality, cell-adhesive Arg-Gly-Asp-Ser (RGDS) peptide is immobilized on the N-(gamma-maleimidobutyryloxy)succinimide-activated P(HEMA-AEMA) hydrogels via reaction of SH with maleimide groups. This modification is especially suitable for the survival of primary chronic lymphocytic leukemia cells (B-CLLs) in 3D cell culture. No other tested stimuli (interleukin-4, CD40 ligand, or shaking) can further improve B-CLL survival or metabolic activity. Both unmodified and RGDS-modified P(HEMA-AEMA) scaffolds serve as a long-term (70 days) 3D culture platforms for HS-5 and M2-10B4 bone marrow stromal cell lines and MEC-1 and HG-3 B-CLL cell lines, although the adherent cells retain their physiological morphologies, preferably on RGDS-modified hydrogels. Moreover, the porosity of hydrogels allows direct cell lysis, followed by efficient DNA isolation from the 3D-cultured cells. P(HEMA-AEMA)-RGDS thus serves as a suitable 3D in vitro leukemia model that enables molecular and metabolic assays and allows imaging of cell morphology, interactions, and migration by confocal microscopy. Such applications can prospectively assist in testing of drugs to treat this frequently recurring or refractory cancer.
ER  -

SVOZILOVÁ, Hana, Z. PLICHTA, V. PROKS, Radana STUDENÁ, Jiří BALOUN, Michael DOUBEK, Šárka POSPÍŠILOVÁ a D. HORAK. RGDS-Modified Superporous Poly(2-Hydroxyethyl Methacrylate)-Based Scaffolds as 3D In Vitro Leukemia Model. \textit{International Journal of Molecular Sciences}. Basel: Multidisciplinary Digital Publishing Institute, 2021, roč.~22, č.~5, s.~2376-2392. ISSN~1422-0067. Dostupné z: https://dx.doi.org/10.3390/ijms22052376.

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