Increased TRPV1 gene expression in the tissues of Barrett's esophagus and esophageal adenocarcinoma

DEISSOVÁ, Tereza, Lydie IZAKOVIČOVÁ HOLLÁ, Zdeněk KALA, Lumír KUNOVSKÝ, Jiří DOLINA, Ondřej SLABÝ and Petra BOŘILOVÁ LINHARTOVÁ. Increased TRPV1 gene expression in the tissues of Barrett's esophagus and esophageal adenocarcinoma. In GASTRO 2021 Prague. 2021.

Basic information

• Original name: Increased TRPV1 gene expression in the tissues of Barrett's esophagus and esophageal adenocarcinoma
• Authors: DEISSOVÁ, Tereza (203 Czech Republic), Lydie IZAKOVIČOVÁ HOLLÁ (203 Czech Republic), Zdeněk KALA (203 Czech Republic), Lumír KUNOVSKÝ (203 Czech Republic), Jiří DOLINA (203 Czech Republic), Ondřej SLABÝ (203 Czech Republic) and Petra BOŘILOVÁ LINHARTOVÁ (203 Czech Republic, guarantor, belonging to the institution).
• Edition: GASTRO 2021 Prague, 2021.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 30101 Human genetics
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14110/21:00120191
• Organization unit: Faculty of Medicine
• Keywords in English: gastroesophageal reflux disease; TRPV1; gene expression; single nucleotide polymorphisms

Abstract

Aim: Gastroesophageal reflux disease (GERD) occurs when the stomach contents return back to the esophagus and causes symptoms and/or complications such as reflux esophagitis (RE), Barrett's esophagus (BE) or esophageal adenocarcinoma (EAC). The transient receptor potential vanilloid subtype 1 (TRPV1) is main acid receptor, whose up-regulation plays an important role in the development of distal esophageal inflammation and reflux symptoms. The aim of this study was to analyze two functional single nucleotide polymorphisms (SNPs) in TRPV1 gene and its expression in esophageal tissue in patients with GERD. Methods: Two SNPs (C/G rs222747; C/T rs8065080) in TRPV1 gene were determined in the 325 patients with GERD (142 with RE; 61 with BE; 22 with EAC) and 101 healthy controls. The TRPV1 mRNA expression was analyzed in pathological and healthy esophageal tissues of 10 patients with RE, 8 with BE and 7 with EAC. Both types of experiments were based on TaqMan® polymerase chain reaction method. For statistical evaluation was used the Statistica v13.2 software. Results: The allele, genotype, haplogenotype or haplotype frequencies of study SNPs between patients with GERD or RE/BE/EAC separately and healthy controls were similar (p>0.05). The higher mRNA TRPV1 expression was found in the tissues of BE (p=0.017) and EAC (p=0.018) compared to healthy tissues. Conclusion: There was found increased mRNA TRPV1 expression in the pathological esophageal tissues of patients with BE or EAC who might benefit with pharmacological modulation of TRPV1 receptor.

Links

• NU20-03-00126, research and development project: Name: Hostitelský mikrobiom ve vztahu k rozvoji Barrettova jícnu a adenokarcinomu jícnu

Investor: Ministry of Health of the CR, Host microbiome in relation to Barrett ́s esophagus and esophageal adenocarcinoma development, Subprogram 1 - standard