Detection of myocardial fibrosis using MRI in rat model

p 2021

Detection of myocardial fibrosis using MRI in rat model

STRAČINA, Tibor, Jiří VITOUŠ, Ondřej MACÍČEK, Lucie KRÁTKÁ, Michal HENDRYCH et. al.

Basic information

Original name

Detection of myocardial fibrosis using MRI in rat model

Name in Czech

Detekce mykardiální fibrózy pomocí MRI v potkaním modelu

Authors

STRAČINA, Tibor, Jiří VITOUŠ, Ondřej MACÍČEK, Lucie KRÁTKÁ, Michal HENDRYCH, Roman PANOVSKÝ, Petr BABULA, Marie NOVÁKOVÁ and Radovan JIŘÍK

Edition

Imaging Principles of Life 2021, 2021

Other information

Language

English

Type of outcome

Vyžádané přednášky

Field of Study

30105 Physiology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Organization unit

Faculty of Medicine

Keywords (in Czech)

myokardiální fibróza; magnetická rezonance; potkaní model

Keywords in English

myocardial fibrosis; magnetic resonance imaging; rat model

Abstract

V originále

Myocardial fibrosis is a clinically important part of cardiac remodelling. It may lead to heart failure and potentially to death. The pathogenesis and management of myocardial fibrosis are intensively studied on both clinical and preclinical levels. The key point in the management is the early detection of fibrosis. Cardiac magnetic resonance imaging (cMRI) is – beside endomyocardial biopsy – a standard method for diagnosis and quantification of myocardial fibrosis. The present project is focused on the validation of cMRI methods for quantification of myocardial fibrosis in the DOCA-salt rat model. Sprague-Dawley rats (6 weeks old) were randomly divided into 2 groups: fibrosis (FIB) and control (CON). In order to induce myocardial fibrosis in the FIB group, unilateral nephrectomy was performed, followed by deoxycorticosterone acetate administration (DOCA; Sigma-Aldrich, USA; 20 mg/week, s.c.) and daily increased intake of NaCl/KCl (0.9% NaCl + 0.3% KCl, p.o. in drinking water) for 3 weeks. In the control group, the sham operation was followed by vehiculum administration (peanut oil; Sigma-Aldrich, USA; 0.2 mL/week s.c.) and no salt supplementation. All rats were scanned using a 9.4T NMR scanner (Bruker Biospin MRI, Ettlingen, Germany) a day before and 14 and 28 days after the surgery with standard anatomical-imaging methods (for quantification of end-systolic, end-diastolic and myocardial volumes and ejection fraction) and with novel cMRI methods developed for quantification of pre- and post-contrast T1 relaxation times and fractional extracellular volume. At the end of the experiments, the myocardial tissue was harvested for histology. The obtained results reflected the expected development of fibrosis in a consistent way. This provides a basis for our follow-up experiments to study cardiac toxicity of selected treatments relevant to clinical applications.

Links

MUNI/A/1246/2020, interní kód MU

Name: Kardiovaskulární systém: od iontového kanálu k celotělovému modelu (Acronym: KAVASYKAMO)

Investor: Masaryk University

Citovat

STRAČINA, Tibor, Jiří VITOUŠ, Ondřej MACÍČEK, Lucie KRÁTKÁ, Michal HENDRYCH, Roman PANOVSKÝ, Petr BABULA, Marie NOVÁKOVÁ and Radovan JIŘÍK. Detection of myocardial fibrosis using MRI in rat model. In Imaging Principles of Life 2021. 2021.

@misc{1815988,
   author = {Stračina, Tibor and Vitouš, Jiří and Macíček, Ondřej and Krátká, Lucie and Hendrych, Michal and Panovský, Roman and Babula, Petr and Nováková, Marie and Jiřík, Radovan},
   booktitle = {Imaging Principles of Life 2021},
   keywords = {myocardial fibrosis; magnetic resonance imaging; rat model},
   language = {eng},
   title = {Detection of myocardial fibrosis using MRI in rat model},
   year = {2021}
}

TY  - SLIDE
ID  - 1815988
AU  - Stračina, Tibor - Vitouš, Jiří - Macíček, Ondřej - Krátká, Lucie - Hendrych, Michal - Panovský, Roman - Babula, Petr - Nováková, Marie - Jiřík, Radovan
PY  - 2021
TI  - Detection of myocardial fibrosis using MRI in rat model
KW  - myocardial fibrosis
KW  - magnetic resonance imaging
KW  - rat model
N2  - Myocardial fibrosis is a clinically important part of cardiac remodelling. It may lead to heart failure and potentially to death. The pathogenesis and management of myocardial fibrosis are intensively studied on both clinical and preclinical levels. The key point in the management is the early detection of fibrosis. Cardiac magnetic resonance imaging (cMRI) is – beside endomyocardial biopsy – a standard method for diagnosis and quantification of myocardial fibrosis. The present project is focused on the validation of cMRI methods for quantification of myocardial fibrosis in the DOCA-salt rat model. Sprague-Dawley rats (6 weeks old) were randomly divided into 2 groups: fibrosis (FIB) and control (CON). In order to induce myocardial fibrosis in the FIB group, unilateral nephrectomy was performed, followed by deoxycorticosterone acetate administration (DOCA; Sigma-Aldrich, USA; 20 mg/week, s.c.) and daily increased intake of NaCl/KCl (0.9% NaCl + 0.3% KCl, p.o. in drinking water) for 3 weeks. In the control group, the sham operation was followed by vehiculum administration (peanut oil; Sigma-Aldrich, USA; 0.2 mL/week s.c.) and no salt supplementation. All rats were scanned using a 9.4T NMR scanner (Bruker Biospin MRI, Ettlingen, Germany) a day before and 14 and 28 days after the surgery with standard anatomical-imaging methods (for quantification of end-systolic, end-diastolic and myocardial volumes and ejection fraction) and with novel cMRI methods developed for quantification of pre- and post-contrast T1 relaxation times and fractional extracellular volume. At the end of the experiments, the myocardial tissue was harvested for histology. The obtained results reflected the expected development of fibrosis in a consistent way. This provides a basis for our follow-up experiments to study cardiac toxicity of selected treatments relevant to clinical applications.
ER  -

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