Další formáty:
BibTeX
LaTeX
RIS
@article{1816444,
author = {Kovařovic, Vojtěch and Sedláček, Ivo and Petráš, Petr and Králová, Stanislava and Mašlaňová, Ivana and Švec, Pavel and NeumannandSchaal, Meina and Botka, Tibor and Gelbíčová, Tereza and Staňková, Eva and Doškař, Jiří and Pantůček, Roman},
article_location = {Basel, Switzerland},
article_number = {1},
doi = {http://dx.doi.org/10.3390/pathogens11010051},
keywords = {laboratory rat; Staphylococcus; Hyicus-Intermedius species group; taxonomy; whole genome sequencing; variable genetic element; genomic island},
language = {eng},
issn = {2076-0817},
journal = {Pathogens},
title = {Staphylococcus ratti sp. nov. isolated from a lab rat},
url = {https://www.mdpi.com/2076-0817/11/1/51},
volume = {11},
year = {2022}
}
TY - JOUR
ID - 1816444
AU - Kovařovic, Vojtěch - Sedláček, Ivo - Petráš, Petr - Králová, Stanislava - Mašlaňová, Ivana - Švec, Pavel - Neumann-Schaal, Meina - Botka, Tibor - Gelbíčová, Tereza - Staňková, Eva - Doškař, Jiří - Pantůček, Roman
PY - 2022
TI - Staphylococcus ratti sp. nov. isolated from a lab rat
JF - Pathogens
VL - 11
IS - 1
SP - 1-16
EP - 1-16
PB - MDPI
SN - 20760817
KW - laboratory rat
KW - Staphylococcus
KW - Hyicus-Intermedius species group
KW - taxonomy
KW - whole genome sequencing
KW - variable genetic element
KW - genomic island
UR - https://www.mdpi.com/2076-0817/11/1/51
N2 - Staphylococci from the Staphylococcus intermedius-Staphylococcus hyicus species group include numerous animal pathogens and are an important reservoir of virulence and antimicrobial resistance determinants. Due to their pathogenic potential, they are possible causative agents of zoonoses in humans; therefore, it is important to address the properties of these strains. Here we used a polyphasic taxonomic approach to characterize the coagulase-negative staphylococcal strain NRL/St 03/464T, isolated from the nostrils of a healthy laboratory rat during a microbiological screening of laboratory animals. The 16S rRNA sequence, MALDI-TOF mass spectrometry and positive urea hydrolysis and beta-glucuronidase tests clearly distinguished it from closely related Staphylococcus spp. All analyses have consistently shown that the closest relative is Staphylococcus chromogenes; however, values of digital DNA-DNA hybridization <35.3% and an average nucleotide identity <81.4% confirmed that the analyzed strain is a distinct Staphylococcus species. Whole-genome sequencing and expert annotation of the genome revealed the presence of novel variable genetic elements, including two plasmids named pSR9025A and pSR9025B, prophages, genomic islands and a composite transposon that may confer selective advantages to other bacteria and enhance their survival. Based on phenotypic, phylogenetic and genomic data obtained in this study, the strain NRL/St 03/464T (= CCM 9025T = LMG 31873T = DSM 111348T) represents a novel species with the suggested name Staphylococcus ratti sp. nov.
ER -
KOVAŘOVIC, Vojtěch, Ivo SEDLÁČEK, Petr PETRÁŠ, Stanislava KRÁLOVÁ, Ivana MAŠLAŇOVÁ, Pavel ŠVEC, Meina NEUMANN-SCHAAL, Tibor BOTKA, Tereza GELBÍČOVÁ, Eva STAŇKOVÁ, Jiří DOŠKAŘ a Roman PANTŮČEK. Staphylococcus ratti sp. nov. isolated from a lab rat. \textit{Pathogens}. Basel, Switzerland: MDPI, 2022, roč.~11, č.~1, s.~1-16. ISSN~2076-0817. Dostupné z: https://dx.doi.org/10.3390/pathogens11010051.
|
