Divergent estimates of the ratio between Na+-Ca2+ current
densities in t-tubular and surface membranes of rat ventricular
cardiomyocytes

J 2021

Divergent estimates of the ratio between Na+-Ca2+ current densities in t-tubular and surface membranes of rat ventricular cardiomyocytes

PÁSEK, Michal, Jiří ŠIMURDA, Markéta BÉBAROVÁ a Georges CHRISTE

Základní údaje

Originální název

Divergent estimates of the ratio between Na+-Ca2+ current densities in t-tubular and surface membranes of rat ventricular cardiomyocytes

Autoři

PÁSEK, Michal (203 Česká republika, garant, domácí), Jiří ŠIMURDA (203 Česká republika, domácí), Markéta BÉBAROVÁ (203 Česká republika, domácí) a Georges CHRISTE (250 Francie)

Vydání

Journal of Cell Science, Cambridge, Company of Biologists Ltd. 2021, 0021-9533

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.235

Kód RIV

RIV/00216224:14110/21:00120203

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1242/jcs.258228

UT WoS

000681395800011

Klíčová slova anglicky

Cardiac myocyte; t-tubules; Na-Ca exchanger; Membrane current density; Log-normal distribution; Monte Carlo simulation

Štítky

14110515, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 1. 2022 07:31, Mgr. Tereza Miškechová

Anotace

V originále

The ratio between Na+-Ca2+ exchange current densities in t-tubular and surface membranes of rat ventricular cardiomyocytes (J(NaCa)-ratio) estimated from electrophysiological data published to date yields strikingly different values between 1.7 and nearly 40. Possible reasons for such divergence were analysed by Monte Carlo simulations assuming both normal and log-normal distribution of the measured data. The confidence intervals CI95 of themean J(NaCa)-ratios computed from the reported data showed an overlap of values between 1 and 3, and between 0.3 and 4.3 in the case of normal and log-normal distribution, respectively. Further analyses revealed that the published high values likely result from a large scatter of data due to transmural differences in J(NaCa), dispersion of cell membrane capacitances and variability in incomplete detubulation. Taking into account the asymmetric distribution of the measured data, the reduction of mean current densities after detubulation and the substantiallysmaller CI95 of lower values of the mean J(NaCa)-ratio, the values between 1.6 and 3.2 may be considered as the most accurate estimates. This implies that 40 to 60% of Na+-Ca2+ exchanger is located at the t-tubular membrane of adult rat ventricular cardiomyocytes.

Návaznosti

NV16-30571A, projekt VaV

Název: Klinický význam a elektrofyziologické zhodnocení mutace c.926C>T genu KCNQ1 (p.T309I) jako možné „founder mutation“ syndromu dlouhého intervalu QT

Citovat

PÁSEK, Michal, Jiří ŠIMURDA, Markéta BÉBAROVÁ a Georges CHRISTE. Divergent estimates of the ratio between Na+-Ca2+ current densities in t-tubular and surface membranes of rat ventricular cardiomyocytes. Journal of Cell Science. Cambridge: Company of Biologists Ltd., 2021, roč. 134, č. 14, s. 1-7. ISSN 0021-9533. Dostupné z: https://dx.doi.org/10.1242/jcs.258228.

@article{1818481,
   author = {Pásek, Michal and Šimurda, Jiří and Bébarová, Markéta and Christe, Georges},
   article_location = {Cambridge},
   article_number = {14},
   doi = {http://dx.doi.org/10.1242/jcs.258228},
   keywords = {Cardiac myocyte; t-tubules; Na-Ca exchanger; Membrane current density; Log-normal distribution; Monte Carlo simulation},
   language = {eng},
   issn = {0021-9533},
   journal = {Journal of Cell Science},
   title = {Divergent estimates of the ratio between Na+-Ca2+ current densities in t-tubular and surface membranes of rat ventricular cardiomyocytes},
   url = {https://journals.biologists.com/jcs/article-abstract/134/14/jcs258228/271058/Divergent-estimates-of-the-ratio-between-Na-Ca2?redirectedFrom=fulltext},
   volume = {134},
   year = {2021}
}

TY  - JOUR
ID  - 1818481
AU  - Pásek, Michal - Šimurda, Jiří - Bébarová, Markéta - Christe, Georges
PY  - 2021
TI  - Divergent estimates of the ratio between Na+-Ca2+ current densities in t-tubular and surface membranes of rat ventricular cardiomyocytes
JF  - Journal of Cell Science
VL  - 134
IS  - 14
SP  - 1-7
EP  - 1-7
PB  - Company of Biologists Ltd.
SN  - 00219533
KW  - Cardiac myocyte
KW  - t-tubules
KW  - Na-Ca exchanger
KW  - Membrane current density
KW  - Log-normal distribution
KW  - Monte Carlo simulation
UR  - https://journals.biologists.com/jcs/article-abstract/134/14/jcs258228/271058/Divergent-estimates-of-the-ratio-between-Na-Ca2?redirectedFrom=fulltext
N2  - The ratio between Na+-Ca2+ exchange current densities in t-tubular and surface membranes of rat ventricular cardiomyocytes (J(NaCa)-ratio) estimated from electrophysiological data published to date yields strikingly different values between 1.7 and nearly 40. Possible reasons for such divergence were analysed by Monte Carlo simulations assuming both normal and log-normal distribution of the measured data. The confidence intervals CI95 of themean J(NaCa)-ratios computed from the reported data showed an overlap of values between 1 and 3, and between 0.3 and 4.3 in the case of normal and log-normal distribution, respectively. Further analyses revealed that the published high values likely result from a large scatter of data due to transmural differences in J(NaCa), dispersion of cell membrane capacitances and variability in incomplete detubulation. Taking into account the asymmetric distribution of the measured data, the reduction of mean current densities after detubulation and the substantiallysmaller CI95 of lower values of the mean J(NaCa)-ratio, the values between 1.6 and 3.2 may be considered as the most accurate estimates. This implies that 40 to 60% of Na+-Ca2+ exchanger is located at the t-tubular membrane of adult rat ventricular cardiomyocytes.
ER  -

PÁSEK, Michal, Jiří ŠIMURDA, Markéta BÉBAROVÁ a Georges CHRISTE. Divergent estimates of the ratio between Na+-Ca2+ current densities in t-tubular and surface membranes of rat ventricular cardiomyocytes. \textit{Journal of Cell Science}. Cambridge: Company of Biologists Ltd., 2021, roč.~134, č.~14, s.~1-7. ISSN~0021-9533. Dostupné z: https://dx.doi.org/10.1242/jcs.258228.

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