Mutually opposing activity of PIN7 splicing isoforms is
required for auxin-mediated tropic responses in Arabidopsis
thaliana

J 2022

Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana

KASHKAN, Ivan, Mónika HRTYAN, Katarzyna RETZER, Jana HUMPOLÍČKOVÁ, Aswathy JAYASREE et. al.

Základní údaje

Originální název

Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana

Autoři

KASHKAN, Ivan (112 Bělorusko, domácí), Mónika HRTYAN (348 Maďarsko, domácí), Katarzyna RETZER, Jana HUMPOLÍČKOVÁ, Aswathy JAYASREE (356 Indie, domácí), Roberta FILEPOVÁ, Zuzana VONDRÁKOVÁ, Sibu SIMON, Debbie ROMBAUT, Thomas B. JACOBS, Mikko J. FRILANDER, Jan HEJÁTKO (203 Česká republika, domácí), Jiří FRIML, Jan PETRÁŠEK a Kamil RŮŽIČKA (203 Česká republika, garant, domácí)

Vydání

New Phytologist, Wiley, 2022, 0028-646X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 9.400

Kód RIV

RIV/00216224:14740/22:00125152

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1111/nph.17792

UT WoS

000714678100001

Klíčová slova anglicky

alternative splicing; Arabidopsis thaliana; auxin; auxin transport; FRAP; PINs; plant development; RNA processing

Štítky

CF CELLIM, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 8. 10. 2024 10:42, Ing. Martina Blahová

Anotace

V originále

Advanced transcriptome sequencing has revealed that the majority of eukaryotic genes undergo alternative splicing (AS). Nonetheless, little effort has been dedicated to investigating the functional relevance of particular splicing events, even those in the key developmental and hormonal regulators. Combining approaches of genetics, biochemistry and advanced confocal microscopy, we describe the impact of alternative splicing on the PIN7 gene in the model plant Arabidopsis thaliana. PIN7 encodes a polarly localized transporter for the phytohormone auxin and produces two evolutionarily conserved transcripts, PIN7a and PIN7b. PIN7a and PIN7b, differing in a four amino acid stretch, exhibit almost identical expression patterns and subcellular localization. We reveal that they are closely associated and mutually influence each other's mobility within the plasma membrane. Phenotypic complementation tests indicate that the functional contribution of PIN7b per se is minor, but it markedly reduces the prominent PIN7a activity, which is required for correct seedling apical hook formation and auxin-mediated tropic responses. Our results establish alternative splicing of the PIN family as a conserved, functionally relevant mechanism, revealing an additional regulatory level of auxin-mediated plant development.

Návaznosti

EF16_013/0001775, projekt VaV

Název: Modernizace a podpora výzkumných aktivit národní infrastruktury pro biologické a medicínské zobrazování Czech-BioImaging

EF16_019/0000738, projekt VaV

Název: Centrum experimentální biologie rostlin

GA16-26428S, projekt VaV

Název: Alternativní sestřih jako regulátor auxinového transportu

Investor: Grantová agentura ČR, Alternativní sestřih jako regulátor auxinového transportu

GA18-26981S, projekt VaV

Název: Genetické studie k objasnění molekulárního mechanizmu účinku strigolaktonů v kořeni

Investor: Grantová agentura ČR, Genetické studie k objasnění molekulárního mechanizmu účinku strigolaktonů v kořeni

90129, velká výzkumná infrastruktura

Název: Czech-BioImaging II

Citovat

KASHKAN, Ivan, Mónika HRTYAN, Katarzyna RETZER, Jana HUMPOLÍČKOVÁ, Aswathy JAYASREE, Roberta FILEPOVÁ, Zuzana VONDRÁKOVÁ, Sibu SIMON, Debbie ROMBAUT, Thomas B. JACOBS, Mikko J. FRILANDER, Jan HEJÁTKO, Jiří FRIML, Jan PETRÁŠEK a Kamil RŮŽIČKA. Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana. New Phytologist. Wiley, 2022, roč. 233, č. 1, s. 329-343. ISSN 0028-646X. Dostupné z: https://dx.doi.org/10.1111/nph.17792.

@article{1819298,
   author = {Kashkan, Ivan and Hrtyan, Mónika and Retzer, Katarzyna and Humpolíčková, Jana and Jayasree, Aswathy and Filepová, Roberta and Vondráková, Zuzana and Simon, Sibu and Rombaut, Debbie and Jacobs, Thomas B. and Frilander, Mikko J. and Hejátko, Jan and Friml, Jiří and Petrášek, Jan and Růžička, Kamil},
   article_number = {1},
   doi = {http://dx.doi.org/10.1111/nph.17792},
   keywords = {alternative splicing; Arabidopsis thaliana; auxin; auxin transport; FRAP; PINs; plant development; RNA processing},
   language = {eng},
   issn = {0028-646X},
   journal = {New Phytologist},
   title = {Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana},
   url = {https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.17792},
   volume = {233},
   year = {2022}
}

TY  - JOUR
ID  - 1819298
AU  - Kashkan, Ivan - Hrtyan, Mónika - Retzer, Katarzyna - Humpolíčková, Jana - Jayasree, Aswathy - Filepová, Roberta - Vondráková, Zuzana - Simon, Sibu - Rombaut, Debbie - Jacobs, Thomas B. - Frilander, Mikko J. - Hejátko, Jan - Friml, Jiří - Petrášek, Jan - Růžička, Kamil
PY  - 2022
TI  - Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana
JF  - New Phytologist
VL  - 233
IS  - 1
SP  - 329-343
EP  - 329-343
PB  - Wiley
SN  - 0028646X
KW  - alternative splicing
KW  - Arabidopsis thaliana
KW  - auxin
KW  - auxin transport
KW  - FRAP
KW  - PINs
KW  - plant development
KW  - RNA processing
UR  - https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.17792
N2  - Advanced transcriptome sequencing has revealed that the majority of eukaryotic genes undergo alternative splicing (AS). Nonetheless, little effort has been dedicated to investigating the functional relevance of particular splicing events, even those in the key developmental and hormonal regulators. Combining approaches of genetics, biochemistry and advanced confocal microscopy, we describe the impact of alternative splicing on the PIN7 gene in the model plant Arabidopsis thaliana. PIN7 encodes a polarly localized transporter for the phytohormone auxin and produces two evolutionarily conserved transcripts, PIN7a and PIN7b. PIN7a and PIN7b, differing in a four amino acid stretch, exhibit almost identical expression patterns and subcellular localization. We reveal that they are closely associated and mutually influence each other's mobility within the plasma membrane. Phenotypic complementation tests indicate that the functional contribution of PIN7b per se is minor, but it markedly reduces the prominent PIN7a activity, which is required for correct seedling apical hook formation and auxin-mediated tropic responses. Our results establish alternative splicing of the PIN family as a conserved, functionally relevant mechanism, revealing an additional regulatory level of auxin-mediated plant development.
ER  -

KASHKAN, Ivan, Mónika HRTYAN, Katarzyna RETZER, Jana HUMPOLÍČKOVÁ, Aswathy JAYASREE, Roberta FILEPOVÁ, Zuzana VONDRÁKOVÁ, Sibu SIMON, Debbie ROMBAUT, Thomas B. JACOBS, Mikko J. FRILANDER, Jan HEJÁTKO, Jiří FRIML, Jan PETRÁŠEK a Kamil RŮŽIČKA. Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana. \textit{New Phytologist}. Wiley, 2022, roč.~233, č.~1, s.~329-343. ISSN~0028-646X. Dostupné z: https://dx.doi.org/10.1111/nph.17792.

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