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@article{1819357,
author = {Mlčochová, Hana and Ratih, Ratih and Michalcová, Lenka and Wätzig, Hermann and Glatz, Zdeněk and Stein, Matthias},
article_number = {16-17},
doi = {http://dx.doi.org/10.1002/elps.202100320},
keywords = {msACE; CE-FA; affinity interaction},
language = {eng},
issn = {0173-0835},
journal = {Electrophoresis},
title = {Comparison of mobility shift affinity capillary electrophoresis and capillary electrophoresis frontal analysis for binding constant determination between human serum albumin and small drugs},
url = {https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/elps.202100320},
volume = {43},
year = {2022}
}
TY - JOUR
ID - 1819357
AU - Mlčochová, Hana - Ratih, Ratih - Michalcová, Lenka - Wätzig, Hermann - Glatz, Zdeněk - Stein, Matthias
PY - 2022
TI - Comparison of mobility shift affinity capillary electrophoresis and capillary electrophoresis frontal analysis for binding constant determination between human serum albumin and small drugs
JF - Electrophoresis
VL - 43
IS - 16-17
SP - 1724-1734
EP - 1724-1734
PB - Wiley
SN - 01730835
KW - msACE
KW - CE-FA
KW - affinity interaction
UR - https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/elps.202100320
N2 - In this study, two capillary electrophoresis–based ligand binding assays, namely, mobility shift affinity capillary electrophoresis (ms-ACE) and capillary electrophoresis-frontal analysis (CE-FA), were applied to determine binding parameters of human serum albumin toward small drugs under similar experimental conditions. The substances S-amlodipine (S-AML), lidocaine (LDC), l-tryptophan (l-TRP), carbamazepine (CBZ), ibuprofen (IBU), and R-verapamil (R-VPM) were used as the main binding partners. The scope of this comparative study was to estimate and compare both the assays in terms of their primary measure's precision and the reproducibility of the derived binding parameters. The effective mobility could be measured with pooled CV values between 0.55% and 7.6%. The precision of the r values was found in the range between 1.5% and 10%. Both assays were not universally applicable. The CE-FA assay could successfully be applied to measure the drugs IBU, CBZ, and LDC, and the interaction toward CBZ, S-AML, l-TRP, and R-VPM could be determined using ms-ACE. The average variabilities of the estimated binding constants were 64% and 67% for CE-FA and ms-ACE, respectively.
ER -
MLČOCHOVÁ, Hana, Ratih RATIH, Lenka MICHALCOVÁ, Hermann WÄTZIG, Zdeněk GLATZ a Matthias STEIN. Comparison of mobility shift affinity capillary electrophoresis and capillary electrophoresis frontal analysis for binding constant determination between human serum albumin and small drugs. \textit{Electrophoresis}. Wiley, 2022, roč.~43, 16-17, s.~1724-1734. ISSN~0173-0835. Dostupné z: https://dx.doi.org/10.1002/elps.202100320.
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