MEIS-WNT5A axis regulates development of fourth ventricle
choroid plexus

J 2021

MEIS-WNT5A axis regulates development of fourth ventricle choroid plexus

KAISER, Karol, Ahram JANG, Petra KOMPANÍKOVÁ, Melody P. LUN, Jan PROCHAZKA et. al.

Základní údaje

Originální název

MEIS-WNT5A axis regulates development of fourth ventricle choroid plexus

Autoři

KAISER, Karol (703 Slovensko, garant, domácí), Ahram JANG, Petra KOMPANÍKOVÁ (703 Slovensko, domácí), Melody P. LUN, Jan PROCHAZKA, Ondrej MACHON, Neil DANI, Michaela PROCHAZKOVA, Benoit LAURENT, Daniel GYLLBORG, Renee VAN AMERONGEN, Ryann M. FAME, Suhasini GUPTA, Feizhen WU, Roger A. BARKER, Ivana BUKOVA, Radislav SEDLACEK, Zbynek KOZMIK, Ernest ARENAS, Maria K. LEHTINEN (203 Česká republika) a Vítězslav BRYJA (203 Česká republika, domácí)

Vydání

Development, Company of Biologists Ltd, 2021, 0950-1991

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10605 Developmental biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.862

Kód RIV

RIV/00216224:14310/21:00123746

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1242/dev.192054

UT WoS

000657670600005

Klíčová slova anglicky

Choroid plexus; Epithelium; Meis1; Meis2; Morphogenesis; WNT5a

Štítky

CF CELLIM, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 14. 1. 2022 17:00, Mgr. Marie Šípková, DiS.

Anotace

V originále

The choroid plexus (ChP) produces cerebrospinal fluid and forms an essential brain barrier. ChP tissues form in each brain ventricle, each one adopting a distinct shape, but remarkably little is known about the mechanisms underlying ChP development. Here, we show that epithelial WNT5A is crucial for determining fourth ventricle (4V) ChP morphogenesis and size in mouse. Systemic Wnt5a knockout, or forced Wnt5a overexpression beginning at embryonic day 10.5, profoundly reduced ChP size and development. However, Wnt5a expression was enriched in Foxj1-positive epithelial cells of 4V ChP plexus, and its conditional deletion in these cells affected the branched, villous morphology of the 4V ChP. We found that WNT5A was enriched in epithelial cells localized to the distal tips of 4V ChP villi, where WNT5A acted locally to activate non-canonical WNT signaling via ROR1 and ROR2 receptors. During 4V ChP development, MEIS1 bound to the proximal Wnt5a promoter, and gain- and loss-of-function approaches demonstrated that MEIS1 regulated Wnt5a expression. Collectively, our findings demonstrate a dual function of WNT5A in ChP development and identify MEIS transcription factors as upstream regulators of Wnt5a in the 4V ChP epithelium.

Návaznosti

LM2018129, projekt VaV

Název: Národní infrastruktura pro biologické a medicínské zobrazování Czech-BioImaging

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, National research infrastructure for biological and medical imaging

Citovat

KAISER, Karol, Ahram JANG, Petra KOMPANÍKOVÁ, Melody P. LUN, Jan PROCHAZKA, Ondrej MACHON, Neil DANI, Michaela PROCHAZKOVA, Benoit LAURENT, Daniel GYLLBORG, Renee VAN AMERONGEN, Ryann M. FAME, Suhasini GUPTA, Feizhen WU, Roger A. BARKER, Ivana BUKOVA, Radislav SEDLACEK, Zbynek KOZMIK, Ernest ARENAS, Maria K. LEHTINEN a Vítězslav BRYJA. MEIS-WNT5A axis regulates development of fourth ventricle choroid plexus. Development. Company of Biologists Ltd, 2021, roč. 148, č. 10, s. "dev192054", 17 s. ISSN 0950-1991. Dostupné z: https://dx.doi.org/10.1242/dev.192054.

@article{1821641,
   author = {Kaiser, Karol and Jang, Ahram and Kompaníková, Petra and Lun, Melody P. and Prochazka, Jan and Machon, Ondrej and Dani, Neil and Prochazkova, Michaela and Laurent, Benoit and Gyllborg, Daniel and van Amerongen, Renee and Fame, Ryann M. and Gupta, Suhasini and Wu, Feizhen and Barker, Roger A. and Bukova, Ivana and Sedlacek, Radislav and Kozmik, Zbynek and Arenas, Ernest and Lehtinen, Maria K. and Bryja, Vítězslav},
   article_number = {10},
   doi = {http://dx.doi.org/10.1242/dev.192054},
   keywords = {Choroid plexus; Epithelium; Meis1; Meis2; Morphogenesis; WNT5a},
   language = {eng},
   issn = {0950-1991},
   journal = {Development},
   title = {MEIS-WNT5A axis regulates development of fourth ventricle choroid plexus},
   url = {https://journals.biologists.com/dev/article/148/10/dev192054/268365/MEIS-WNT5A-axis-regulates-development-of-fourth},
   volume = {148},
   year = {2021}
}

TY  - JOUR
ID  - 1821641
AU  - Kaiser, Karol - Jang, Ahram - Kompaníková, Petra - Lun, Melody P. - Prochazka, Jan - Machon, Ondrej - Dani, Neil - Prochazkova, Michaela - Laurent, Benoit - Gyllborg, Daniel - van Amerongen, Renee - Fame, Ryann M. - Gupta, Suhasini - Wu, Feizhen - Barker, Roger A. - Bukova, Ivana - Sedlacek, Radislav - Kozmik, Zbynek - Arenas, Ernest - Lehtinen, Maria K. - Bryja, Vítězslav
PY  - 2021
TI  - MEIS-WNT5A axis regulates development of fourth ventricle choroid plexus
JF  - Development
VL  - 148
IS  - 10
SP  - "dev192054"
EP  - "dev192054"
PB  - Company of Biologists Ltd
SN  - 09501991
KW  - Choroid plexus
KW  - Epithelium
KW  - Meis1
KW  - Meis2
KW  - Morphogenesis
KW  - WNT5a
UR  - https://journals.biologists.com/dev/article/148/10/dev192054/268365/MEIS-WNT5A-axis-regulates-development-of-fourth
N2  - The choroid plexus (ChP) produces cerebrospinal fluid and forms an essential brain barrier. ChP tissues form in each brain ventricle, each one adopting a distinct shape, but remarkably little is known about the mechanisms underlying ChP development. Here, we show that epithelial WNT5A is crucial for determining fourth ventricle (4V) ChP morphogenesis and size in mouse. Systemic Wnt5a knockout, or forced Wnt5a overexpression beginning at embryonic day 10.5, profoundly reduced ChP size and development. However, Wnt5a expression was enriched in Foxj1-positive epithelial cells of 4V ChP plexus, and its conditional deletion in these cells affected the branched, villous morphology of the 4V ChP. We found that WNT5A was enriched in epithelial cells localized to the distal tips of 4V ChP villi, where WNT5A acted locally to activate non-canonical WNT signaling via ROR1 and ROR2 receptors. During 4V ChP development, MEIS1 bound to the proximal Wnt5a promoter, and gain- and loss-of-function approaches demonstrated that MEIS1 regulated Wnt5a expression. Collectively, our findings demonstrate a dual function of WNT5A in ChP development and identify MEIS transcription factors as upstream regulators of Wnt5a in the 4V ChP epithelium.
ER  -

KAISER, Karol, Ahram JANG, Petra KOMPANÍKOVÁ, Melody P. LUN, Jan PROCHAZKA, Ondrej MACHON, Neil DANI, Michaela PROCHAZKOVA, Benoit LAURENT, Daniel GYLLBORG, Renee VAN AMERONGEN, Ryann M. FAME, Suhasini GUPTA, Feizhen WU, Roger A. BARKER, Ivana BUKOVA, Radislav SEDLACEK, Zbynek KOZMIK, Ernest ARENAS, Maria K. LEHTINEN a Vítězslav BRYJA. MEIS-WNT5A axis regulates development of fourth ventricle choroid plexus. \textit{Development}. Company of Biologists Ltd, 2021, roč.~148, č.~10, s.~''dev192054'', 17 s. ISSN~0950-1991. Dostupné z: https://dx.doi.org/10.1242/dev.192054.

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