Cytokine-chemokine profiles in the hippocampus of patients with
mesial temporal lobe epilepsy and hippocampal sclerosis

J 2022

Cytokine-chemokine profiles in the hippocampus of patients with mesial temporal lobe epilepsy and hippocampal sclerosis

AULICKÁ, Štefánia, Katarína ČESKÁ, Jiří ŠÁNA, František SIEGL, Eva BRICHTOVÁ et. al.

Základní údaje

Originální název

Cytokine-chemokine profiles in the hippocampus of patients with mesial temporal lobe epilepsy and hippocampal sclerosis

Autoři

AULICKÁ, Štefánia (703 Slovensko, domácí), Katarína ČESKÁ (703 Slovensko, domácí), Jiří ŠÁNA (203 Česká republika, domácí), František SIEGL (203 Česká republika, domácí), Eva BRICHTOVÁ (203 Česká republika, domácí), Hana OŠLEJŠKOVÁ (203 Česká republika, domácí), Markéta HERMANOVÁ (203 Česká republika, domácí), Michal HENDRYCH (203 Česká republika, domácí), Elleni MICHU (203 Česká republika, domácí), Milan BRÁZDIL (203 Česká republika, domácí), Ondřej SLABÝ (203 Česká republika, domácí) a Igor NESTRAŠIL (203 Česká republika)

Vydání

Epilepsy Research, Amsterdam, Elsevier, 2022, 0920-1211

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30210 Clinical neurology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.200

Kód RIV

RIV/00216224:14110/22:00129659

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1016/j.eplepsyres.2022.106858

UT WoS

000820113600007

Klíčová slova anglicky

Temporal lobe epilepsy; Hippocampal sclerosis; Pharmaco-resistant; Cytokine; Chemokine; Interleukin; Immune response; Epileptogenesis

Štítky

14110112, 14110127, 14110131, 14110320, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 2. 2023 12:58, Mgr. Tereza Miškechová

Anotace

V originále

Purpose Mesial temporal lobe epilepsy with hippocampal sclerosis (MTLE-HS) is the most common drug-resistant epilepsy. Despite major advances in epilepsy research, the epileptogenesis of the MTLE-HS is not well understood. The altered neuroimmune response is one of the pathomechanisms linked to progressive epileptogenesis in MTLE-HS, and understanding its role may help design future cures for pharmaco-resistant MTLE-HS. Here, the neuroimmune function was evaluated by the assessment of cytokine-chemokine profiles in brain samples from the hippocampus of patients with MTLE-HS. Methods Brain samples from patients with MTLE-HS collected during epileptosurgical resection (n = 21) were compared to those obtained from autopsy controls (n = 13). The typing of HS was performed according to ILAE consensus classification, and patients were additionally sorted into subgroups based on the severity of neuronal depletion (Wyler grading system). Differences between patients with MTLE-HS with and without a history of febrile seizures were also assessed. RNA was isolated from native samples, and real-time gene expression analysis of cytokine-chemokine profiles, i.e., levels of IL-1β, IL-6, IL-10, IL-18, CCL2, CCL3, CCL4, and STAT3, was carried out by qRT-PCR methodology. Results Upregulation of IL-1β (p = 0.001), IL-18 (p = 0.0018), CCL2 (p = 0,0377), CCL3 (p < 0.001), and CCL4 (p < 0.001) in MTLE-HS patients was detected when compared to the post-mortem hippocampal samples collected from autopsy controls. The STAT3 expression was higher in more severe neuronal loss and glial scaring determined by different Wyler grades in HS patients. Furthermore, cytokine-chemokine profiles were not different in MTLE-HS patients with or without febrile seizures. Conclusion The upregulation of specific cytokines and chemokines in MTLE-HS provides evidence that the neuroinflammatory process contributes to MTLE epileptogenesis. History of febrile seizures did not alter the immune profiles. Specific immune mediators and related immune pathways represent potential therapeutic targets for seizure control and pharmacoresistancy prevention in MTLE associated with hippocampal sclerosis.

Návaznosti

NU21-04-00305, projekt VaV

Název: Analýza transkriptomu a metylace DNA u pacientů s fokální kortikální dysplázií

Investor: Ministerstvo zdravotnictví ČR, Analýza transkriptomu a metylace DNA u pacientů s fokální kortikální dysplázií, Podprogram 1 - standardní

Citovat

AULICKÁ, Štefánia, Katarína ČESKÁ, Jiří ŠÁNA, František SIEGL, Eva BRICHTOVÁ, Hana OŠLEJŠKOVÁ, Markéta HERMANOVÁ, Michal HENDRYCH, Elleni MICHU, Milan BRÁZDIL, Ondřej SLABÝ a Igor NESTRAŠIL. Cytokine-chemokine profiles in the hippocampus of patients with mesial temporal lobe epilepsy and hippocampal sclerosis. Epilepsy Research. Amsterdam: Elsevier, 2022, roč. 180, February 2022, s. 1-8. ISSN 0920-1211. Dostupné z: https://dx.doi.org/10.1016/j.eplepsyres.2022.106858.

@article{1822741,
   author = {Aulická, Štefánia and Česká, Katarína and Šána, Jiří and Siegl, František and Brichtová, Eva and Ošlejšková, Hana and Hermanová, Markéta and Hendrych, Michal and Michu, Elleni and Brázdil, Milan and Slabý, Ondřej and Nestrašil, Igor},
   article_location = {Amsterdam},
   article_number = {February 2022},
   doi = {http://dx.doi.org/10.1016/j.eplepsyres.2022.106858},
   keywords = {Temporal lobe epilepsy; Hippocampal sclerosis; Pharmaco-resistant; Cytokine; Chemokine; Interleukin; Immune response; Epileptogenesis},
   language = {eng},
   issn = {0920-1211},
   journal = {Epilepsy Research},
   title = {Cytokine-chemokine profiles in the hippocampus of patients with mesial temporal lobe epilepsy and hippocampal sclerosis},
   url = {https://www.sciencedirect.com/science/article/pii/S0920121122000092?via%3Dihub#!},
   volume = {180},
   year = {2022}
}

TY  - JOUR
ID  - 1822741
AU  - Aulická, Štefánia - Česká, Katarína - Šána, Jiří - Siegl, František - Brichtová, Eva - Ošlejšková, Hana - Hermanová, Markéta - Hendrych, Michal - Michu, Elleni - Brázdil, Milan - Slabý, Ondřej - Nestrašil, Igor
PY  - 2022
TI  - Cytokine-chemokine profiles in the hippocampus of patients with mesial temporal lobe epilepsy and hippocampal sclerosis
JF  - Epilepsy Research
VL  - 180
IS  - February 2022
SP  - 1-8
EP  - 1-8
PB  - Elsevier
SN  - 09201211
KW  - Temporal lobe epilepsy
KW  - Hippocampal sclerosis
KW  - Pharmaco-resistant
KW  - Cytokine
KW  - Chemokine
KW  - Interleukin
KW  - Immune response
KW  - Epileptogenesis
UR  - https://www.sciencedirect.com/science/article/pii/S0920121122000092?via%3Dihub#!
N2  - Purpose Mesial temporal lobe epilepsy with hippocampal sclerosis (MTLE-HS) is the most common drug-resistant epilepsy. Despite major advances in epilepsy research, the epileptogenesis of the MTLE-HS is not well understood. The altered neuroimmune response is one of the pathomechanisms linked to progressive epileptogenesis in MTLE-HS, and understanding its role may help design future cures for pharmaco-resistant MTLE-HS. Here, the neuroimmune function was evaluated by the assessment of cytokine-chemokine profiles in brain samples from the hippocampus of patients with MTLE-HS. Methods Brain samples from patients with MTLE-HS collected during epileptosurgical resection (n = 21) were compared to those obtained from autopsy controls (n = 13). The typing of HS was performed according to ILAE consensus classification, and patients were additionally sorted into subgroups based on the severity of neuronal depletion (Wyler grading system). Differences between patients with MTLE-HS with and without a history of febrile seizures were also assessed. RNA was isolated from native samples, and real-time gene expression analysis of cytokine-chemokine profiles, i.e., levels of IL-1β, IL-6, IL-10, IL-18, CCL2, CCL3, CCL4, and STAT3, was carried out by qRT-PCR methodology. Results Upregulation of IL-1β (p = 0.001), IL-18 (p = 0.0018), CCL2 (p = 0,0377), CCL3 (p < 0.001), and CCL4 (p < 0.001) in MTLE-HS patients was detected when compared to the post-mortem hippocampal samples collected from autopsy controls. The STAT3 expression was higher in more severe neuronal loss and glial scaring determined by different Wyler grades in HS patients. Furthermore, cytokine-chemokine profiles were not different in MTLE-HS patients with or without febrile seizures. Conclusion The upregulation of specific cytokines and chemokines in MTLE-HS provides evidence that the neuroinflammatory process contributes to MTLE epileptogenesis. History of febrile seizures did not alter the immune profiles. Specific immune mediators and related immune pathways represent potential therapeutic targets for seizure control and pharmacoresistancy prevention in MTLE associated with hippocampal sclerosis.
ER  -

AULICKÁ, Štefánia, Katarína ČESKÁ, Jiří ŠÁNA, František SIEGL, Eva BRICHTOVÁ, Hana OŠLEJŠKOVÁ, Markéta HERMANOVÁ, Michal HENDRYCH, Elleni MICHU, Milan BRÁZDIL, Ondřej SLABÝ a Igor NESTRAŠIL. Cytokine-chemokine profiles in the hippocampus of patients with mesial temporal lobe epilepsy and hippocampal sclerosis. \textit{Epilepsy Research}. Amsterdam: Elsevier, 2022, roč.~180, February 2022, s.~1-8. ISSN~0920-1211. Dostupné z: https://dx.doi.org/10.1016/j.eplepsyres.2022.106858.

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