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@article{1822753,
author = {Zavesky, Ludek and Jandáková, Eva and Weinberger, Vít and Minář, Luboš and Hanzikova, Veronika and Duskova, Daniela and Faridova, Adela and Turyna, Radovan and Slanar, Ondrej and Horinek, Ales and Kohoutova, Milada},
article_location = {DORDRECHT},
article_number = {3},
doi = {http://dx.doi.org/10.1007/s11033-021-07010-8},
keywords = {Biomarker; Breast cancer; microRNA; miR-451a; miR-548b-5p; U6 snRNA},
language = {eng},
issn = {0301-4851},
journal = {MOLECULAR BIOLOGY REPORTS},
title = {Small non-coding RNA profiling in breast cancer: plasma U6 snRNA, miR-451a and miR-548b-5p as novel diagnostic and prognostic biomarkers},
url = {https://link.springer.com/article/10.1007%2Fs11033-021-07010-8},
volume = {49},
year = {2022}
}
TY - JOUR
ID - 1822753
AU - Zavesky, Ludek - Jandáková, Eva - Weinberger, Vít - Minář, Luboš - Hanzikova, Veronika - Duskova, Daniela - Faridova, Adela - Turyna, Radovan - Slanar, Ondrej - Horinek, Ales - Kohoutova, Milada
PY - 2022
TI - Small non-coding RNA profiling in breast cancer: plasma U6 snRNA, miR-451a and miR-548b-5p as novel diagnostic and prognostic biomarkers
JF - MOLECULAR BIOLOGY REPORTS
VL - 49
IS - 3
SP - 1955-1971
EP - 1955-1971
PB - Springer
SN - 03014851
KW - Biomarker
KW - Breast cancer
KW - microRNA
KW - miR-451a
KW - miR-548b-5p
KW - U6 snRNA
UR - https://link.springer.com/article/10.1007%2Fs11033-021-07010-8
N2 - Background Breast cancer is a leading cause of cancer-related death in women. Most cases are invasive ductal carcinomas of no special type (NST breast carcinomas). Methods and Results In this prospective, multicentric biomarker discovery study, we analyzed the expression of small non-coding RNAs (mainly microRNAs) in plasma by qPCR and evaluated their association with NST breast cancer. Large-scale expression profiling and subsequent validations have been performed in patient and control groups and compared with clinicopathological data. Small nuclear U6 snRNA, miR-548b-5p and miR-451a have been identified as candidate biomarkers. U6 snRNA was remarkably overexpressed in all the validations, miR-548b-5p levels were generally elevated and miR-451a expression was mostly downregulated in breast cancer groups. Combined U6 snRNA/miR-548b-5p signature demonstrated the best diagnostic performance based on the ROC curve analysis with AUC of 0.813, sensitivity 73.1% and specificity 82.6%. There was a trend towards increased expression of both miR-548b-5p and U6 snRNA in more advanced stages. Further, increased miR-548b-5p levels have been partially associated with higher grades, multifocality, Ki-67 positivity, and luminal B rather than luminal A samples. On the other hand, an association has been observed between high miR-451a expression and progesterone receptor positivity, lower grade, unifocal samples, Ki-67-negativity, luminal A rather than luminal B samples as well as improved progression-free survival and overall survival. Conclusions Our results indicated that U6 snRNA and miR-548b-5p may have pro-oncogenic functions, while miR-451a may act as tumor suppressor in breast cancer.
ER -
ZAVESKY, Ludek, Eva JANDÁKOVÁ, Vít WEINBERGER, Luboš MINÁŘ, Veronika HANZIKOVA, Daniela DUSKOVA, Adela FARIDOVA, Radovan TURYNA, Ondrej SLANAR, Ales HORINEK and Milada KOHOUTOVA. Small non-coding RNA profiling in breast cancer: plasma U6 snRNA, miR-451a and miR-548b-5p as novel diagnostic and prognostic biomarkers. \textit{MOLECULAR BIOLOGY REPORTS}. DORDRECHT: Springer, 2022, vol.~49, No~3, p.~1955-1971. ISSN~0301-4851. Available from: https://dx.doi.org/10.1007/s11033-021-07010-8.
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